Biochemical Profile and Genetic Polymorphism of MTHFRC677T in Risk of Type 2 Diabetes Mellituss
International Journal of Diabetes and Endocrinology
Volume 2, Issue 2, June 2017, Pages: 19-25
Received: Dec. 26, 2016; Accepted: Mar. 17, 2017; Published: Apr. 11, 2017
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Authors
Mushtaq Ahmad Bhat, Clinical-Biochemistry, University of Kashmir, Srinagar, India
Showkat Ahmad Bhat, Division of Vety. Biochemistry, F. V. Sc & AH, SKUAST-K, Shuhama Alustang, Srinagar, India
Sheikh Bilal Ahmad, Division of Vety. Biochemistry, F. V. Sc & AH, SKUAST-K, Shuhama Alustang, Srinagar, India
Wasim Qureshi, Registrar Academics/Physician, Govt. Medical College, Srinagar, India
Sabhiya Majid, Department of Biochemistry, Govt. Medical College, Srinagar, India
Aarif Ali, Clinical-Biochemistry, University of Kashmir, Srinagar, India
Ishraq Hussain, Division of Vety. Biochemistry, F. V. Sc & AH, SKUAST-K, Shuhama Alustang, Srinagar, India
Tehseen Hassan, Department of Biochemistry, Govt. Medical College, Srinagar, India
Muneeb U. Rehman, Division of Vety. Biochemistry, F. V. Sc & AH, SKUAST-K, Shuhama Alustang, Srinagar, India
Manzoor R. Mir, Division of Vety. Biochemistry, F. V. Sc & AH, SKUAST-K, Shuhama Alustang, Srinagar, India
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Abstract
Diabetes mellitus (DM) is a common endocrine metabolic disorder and a leading cause of death worldwide Diabetes type-2 is a multicausal disease which develops slowly and in a stepwise order. Our study showed there was no significant difference in serum high density lipoprotein (HDL) and Tri glyceride (TG) of patients and controls (0.90±0.59 vs 1.15±0.39 p>0.05) and (1.19±0.70 vs 1.01±0.52 p>0.060) respectively. Low density lipoprotein (LDL) and total cholesterol (TC) are significantly higher in patients than control group (4.09±1.14 vs 3.01±1.02 p<0.0002) and (4.21±1.28 vs 3.78±1.29 p<0.05). However, HDL/TC ratio is significantly higher in patients than controls (0.21±0.91 vs 0.30±0.99 p<0.05). Serum levels of all liver enzymes (ALT, AST, and ALP) analyzed are significantly higher in patients than controls (12.69±10.80 vs 4.95±2.66, p<0.0002), (15.99±10.70 vs 6.95±3.84, p<0.0002) and 68.29±27.78 vs 21.27±7.77, p<0.0001) respectively. On genetic level the role of MTHFR C677T polymorphisms our results showed 63% of the cases showed homozygous mutant condition. The allelic association of polymorphism of controls with cases was found to be significant (P=0.007). Homozygous mutant condition of MTHFRC677T gene was found to be certainly higher in Diabetes Mellitus 2 Cases of above 60 years of age (80%), than ages below 60 years and in controls (16.6%) and was significant as p=0.005, compared to below 60 years of age (33.3%) and in controls (0%) and association was insignificant as p=0.4667. Our data suggest that there is an important role of LDL, TC, HDL/TC, ALT, AST, and ALP in type-2 Diabetes, also gene polymorphisms of MTHFR C677T gene may act synergistically to increase the risk of type 2 diabetes.
Keywords
Diabetes Mellitus-2, Biochemical Parameters, MTHFR Gene Polymorphism
To cite this article
Mushtaq Ahmad Bhat, Showkat Ahmad Bhat, Sheikh Bilal Ahmad, Wasim Qureshi, Sabhiya Majid, Aarif Ali, Ishraq Hussain, Tehseen Hassan, Muneeb U. Rehman, Manzoor R. Mir, Biochemical Profile and Genetic Polymorphism of MTHFRC677T in Risk of Type 2 Diabetes Mellituss, International Journal of Diabetes and Endocrinology. Vol. 2, No. 2, 2017, pp. 19-25. doi: 10.11648/j.ijde.20170202.11
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Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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