Determination of the Concentration of Polyamines with SPR-Based Immune Biosensor for Early Diagnostics of Breast Cancer
International Journal of Clinical Oncology and Cancer Research
Volume 1, Issue 1, December 2016, Pages: 30-35
Received: Nov. 13, 2016;
Accepted: Dec. 3, 2016;
Published: Jan. 17, 2017
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Maksym Prylutskyi, Department of Laboratory Diagnostics of Biological Systems, Center of Molecular and Cell Research of National University of Kyiv-Mohyla Academy, Kyiv, Ukraine
Nickolaj Starodub, Department of Molecular Biology, Microbiology and Biosafety of National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine
Nadiia Bilko, Department of Laboratory Diagnostics of Biological Systems, Center of Molecular and Cell Research of National University of Kyiv-Mohyla Academy, Kyiv, Ukraine
The paper presents the results of research on the development of immune biosensor test system for express detection of polyamines in cells of breast cancer. Determination of polyamines was performed by using an analytical device - immune biosensor based on surface plasmon resonance (SPR), where "antigen-antibody" reaction is performed in real time on the surface of transducer, resulting in formation of immune complexes and recording the shift of resonance angle. It was performed two methods of analysis: direct, when immobilized specific antibodies interacted with appropriate antigens and competitive, when the one part of antigens was conjugated with protein and competed for binding with specific antibodies with free antigens. In relation to direct analysis it was shown the dependence of sensitivity of biosensor response from the concentration of polyamines in range of 10-100 ng /ml. Concerning the competitive method of analysis, the linear region of sensitivity was in range of 0,3-80 ng/ml and 0,01-90 ng/ml for spermine and spermidine respectively.
Determination of the Concentration of Polyamines with SPR-Based Immune Biosensor for Early Diagnostics of Breast Cancer, International Journal of Clinical Oncology and Cancer Research.
Vol. 1, No. 1,
2016, pp. 30-35.
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