Determination of the Concentration of Polyamines with SPR-Based Immune Biosensor for Early Diagnostics of Breast Cancer
International Journal of Clinical Oncology and Cancer Research
Volume 1, Issue 1, December 2016, Pages: 30-35
Received: Nov. 13, 2016; Accepted: Dec. 3, 2016; Published: Jan. 17, 2017
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Maksym Prylutskyi, Department of Laboratory Diagnostics of Biological Systems, Center of Molecular and Cell Research of National University of Kyiv-Mohyla Academy, Kyiv, Ukraine
Nickolaj Starodub, Department of Molecular Biology, Microbiology and Biosafety of National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine
Nadiia Bilko, Department of Laboratory Diagnostics of Biological Systems, Center of Molecular and Cell Research of National University of Kyiv-Mohyla Academy, Kyiv, Ukraine
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The paper presents the results of research on the development of immune biosensor test system for express detection of polyamines in cells of breast cancer. Determination of polyamines was performed by using an analytical device - immune biosensor based on surface plasmon resonance (SPR), where "antigen-antibody" reaction is performed in real time on the surface of transducer, resulting in formation of immune complexes and recording the shift of resonance angle. It was performed two methods of analysis: direct, when immobilized specific antibodies interacted with appropriate antigens and competitive, when the one part of antigens was conjugated with protein and competed for binding with specific antibodies with free antigens. In relation to direct analysis it was shown the dependence of sensitivity of biosensor response from the concentration of polyamines in range of 10-100 ng /ml. Concerning the competitive method of analysis, the linear region of sensitivity was in range of 0,3-80 ng/ml and 0,01-90 ng/ml for spermine and spermidine respectively.
SPR, Polyamines, Antibody, Antigen, Surface Plasmon Resonance, Breast Cancer
To cite this article
Maksym Prylutskyi, Nickolaj Starodub, Nadiia Bilko, Determination of the Concentration of Polyamines with SPR-Based Immune Biosensor for Early Diagnostics of Breast Cancer, International Journal of Clinical Oncology and Cancer Research. Vol. 1, No. 1, 2016, pp. 30-35. doi: 10.11648/j.ijcocr.20160101.15
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O. Y. Mayevkyi, Breast cancer. Modern Methods of Diagnosis Using Oncomarkers, Specific Immunotherapy., Journal of Vinnytsya National Pirogov Memorial Medical University., vol. 18, 2, 2014, pp. 635-640.
S. P. Osynskyi Metabolic environment of cancer cells., Oncology, 2010, pp. 197–218.
Qi С, Gao G, Jin G. Label-free Biosensors for Health Applications. In book “Biosensors for Health, Environment and Biosecurity”, edited by Pier Andrea Serra. InTech 2011, 550 p.
N. F. Starodub. Efficiency of Biosensors in Environmental Monitoring. Book of SERIES IN SENSORS, Portable Biosensing of Food Toxicants and Environmental Pollutants, CRC Press, Taylor & Francis Croup Boca Raton London, NewYork, 2013, pp. 515-560.
N. S. Sergeeva,, N. V. Marshutina. The general idea of the serologic biomarkers and their place in oncology., Practical Oncology, vol. 12, 4, 2011, pp. 147-154.
V. F Suhoy, V. S. Pervyi, N. V. Suhaya. Tumor markers in clinical practice: Handbook for doctors., Dnepropetrovsk: ART Press, 2003. p. 44.
A. Gugliucci. Polyamines as clinical laboratory tools., Clinica chimica acta 344.1, 2004, 23-35.
M. H. Zhang, T. Caragine, HC Wang, PS Cohen, G. Botchkina, K. Soda, M. Bianchi, P. Ulrich, A. Cerami, B. Sherry, KJ Tracey J. Exp. Med., 185, 1997, p. 1759.
N. Minois, D. Carmona-Gutierrez, F. Madeo Aging, 3, 2011, p. 716.
V. G. Brunton, MH Grant, HM WallaceBiochem. Pharmacol., 40, 1990, p. 1893.
D. M. L. Morgan Biochem. Soc. Trans., 18, 1990, p. 1080.
D. H. Russell Nat. New Biol., 233, 1971, p. 144.
T. Takayama et al. Diagnostic approach to breast cancer patients based on target metabolomics in saliva by liquid chromatography with tandem mass spectrometry Clinica Chimica Acta, 452, 2016, pp. 18–26.
L. D Voronchihina, V. T Demyanova, S. A Sitnikov. Polyamine concentration in the blood of healthy people. Problems of Medical Chemistry., vol 22., 2., 1986, pp. 43-45.
D. H. Russell, S. H. Snyder, Amine synthesis in rapidly growing tissues: ornithine decarboxylase activity in regenerating rat liver, chick embryo, and various tumors, Proc. Natl. Acad. Sci. 60, 1968, pp. 1420–1427.
J. W. Suh, S. H. Lee, B. C. Chung, J. Park, Urinary polyamine evaluation for effective diagnosis of various cancers, J Chromatogr. B 688, 1997, pp. 179–186.
S. H. Lee, S. O. Kim, H. Lee, B. C. Chung, Estrogens and polyamines in breast cancer: their profiles and values in disease staging, Cancer Lett. 133, 1998, pp. 47–56.
K. Hiramatsu, H. Miura, S. Kamei, K. Iwasaki, M. Kawakita, Development of a sensitive and accurate enzyme-linked immunosorbent assay (ELISA) system that can replace HPLC analysis for the determination of N1, N12-diacetylspermine in human urine, J. Biochem. 124,1998, pp. 231–236.
D Bartos,., R. A Campbell,., F Bartos,., D. P Grettie,. Direct determination of polyamines in human serum by radioimmunoassay. J. Cancer Res. 35, 1975, pp. 2056-2060.
Fujiwara, K et al.,. Preparation ofpolyamine antibody and its use in enzyme immunoassay of spermine and spermidine with `D-galactosidase as a label. J. Immunol. Methods 61, 1983, pp. 217-226.
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