Study on the Survival and Reproductive Performance of Sterile Male Glossina pallidipes
International Journal of Biomedical Science and Engineering
Volume 4, Issue 3, June 2016, Pages: 13-21
Received: May 6, 2016; Accepted: May 20, 2016; Published: Jun. 30, 2016
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Authors
Tadese Setegn, Ethiopian Veterinary Drug and Animal feed Administration and Control Authority, Addis Ababa, Ethiopia
Tesfu Fekensa, Ethiopian Biodiversity Institute, Addis Ababa, Ethiopia
Emiru Siyoum, Addis Ababa University, College of Natural Sciences, Addis Ababa, Ethiopia
Solomon Mekonnen, Kality Tsetse Fly Rearing and Irradiation Center, Addis Ababa, Ethiopia
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Abstract
Laboratory investigation was conducted to know the effect of irradiation (sterility) on reproductive performance of females G. pallidipes mated with sterile and fertile males under temperature of 23-25°C and 75-80% relative humidity. Completely randomized designs with three replications were followed throughout the experiments. The irradiation effects on male G. pallidipes at different doses of irradiation were checked using, 60Gy, 80Gy, 100Gy and 120Gy. Results showed that at highest doses the sterility effects on male Glossina pallidipes were significantly lower than lowest doses (P<0.05) on pupae production and emergence of adults. On the other hand, the highest dose (120Gy) caused abortion of egg/larvae significantly higher than the lower dose (60Gy) (P<0.05). In other investigation which was aimed to assessing the competitiveness of sterile malestofertile males in a cage at ratios of 9 sterile: 1 fertile males, 7 sterile to 3 fertile males, 5 sterile to 5 fertile males and 3 sterile to 7 fertile males, the results showed that the ratio with the highest number of sterile (9:1) in the cage caused significantly low number of pupae and number of adults emerged from pupae than those with lower number of sterile males (3:7) (P<0.05). Similarly, the ratio with the highest sterile males (9:1) resulted in significantly higher number of egg/larvae abortion than those with lower number of sterile males (3:7) ratio (P<0.05). In the present study, survival of male G. pallidipes irradiated with 120Gy three days after emergence from pupae was significantly higher than males irradiatedone and ten days after emergence from pupae (P<0.05). This may indicate that irradiated males may need to rest up to 7 or 10 days before released to the field. The importance of sterilization using appropriate dose, the appropriate ratio of sterile to fertile males and the time at which sterility was done efficiently all are relevant.
Keywords
Tsetse Fly, Trypanosomosis, SIT, Irradiation and Kality
To cite this article
Tadese Setegn, Tesfu Fekensa, Emiru Siyoum, Solomon Mekonnen, Study on the Survival and Reproductive Performance of Sterile Male Glossina pallidipes, International Journal of Biomedical Science and Engineering. Vol. 4, No. 3, 2016, pp. 13-21. doi: 10.11648/j.ijbse.20160403.11
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Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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