American Journal of Clinical and Experimental Medicine
Volume 3, Issue 1-1, February 2015, Pages: 29-32
Received: Dec. 24, 2014;
Accepted: Dec. 28, 2014;
Published: Feb. 15, 2015
Views 3174 Downloads 164
Polycarp Chia, Department of Biochemistry, Catholic University of Cameroon, Bamenda, Cameroon
Elvis Fon Tatah, Science for Life Foundation, Bamenda, Cameroon
Kenneth Yongabi, Phyto-Biotechnology Research Foundation Institute, Catholic University of Cameroon, Bamenda, Cameroon
EBOV share many symptoms with a lot of common diseases, so only a well trained Laboratorian can properly collect samples, handle, do differential diagnosis and contain the disease. Quality assurance and control should be on-going in medical Laboratyories. BSL-4 laboratories should be built in African countries where this disease is prevalent. The government should develop a maintenance culture for the infrastructure. Maximum containment is needed when working with this virus as 90% of infected people die.
Elvis Fon Tatah,
Laboratory Diagnostic Tools for Checking Ebola Viral Infections in Africa, American Journal of Clinical and Experimental Medicine. Special Issue: Clinical Innovations, Developments in the Diagnosis, Management and Prevention of Ebola Disease (Marburg fever) and Hemorrhagic Fevers.
Vol. 3, No. 1-1,
2015, pp. 29-32.
Australian Public Health Laboratory Network: Laboratory Precautions for Samples Collected from Patients with Suspected Viral Haemorrhagic Fevers
CDC: Interim Guidance for Managing Patients with Suspected Viral Haemorrhagic Fever in U.S. Hospitals
Ebola Virus 2014 West Africa Outbreak – CDC website link
Borio, L., T. Inglesby, C. J. Peters, A. L. Schmaljohn, J. M. Hughes, P. B. Jahrling, T. Ksiazek, K. M. Johnson, A. Meyerhoff, T. O'Toole, M. S. Ascher, J. Bartlett, J. G. Breman, E. M. Eitzen, Jr., M. Hamburg, J. Hauer, D. A. Henderson, R. T. Johnson, G. Kwik, M. Layton, S. Lillibridge, G. J. Nabel, M. T. Osterholm, T. M. Perl, P. Russell, and K. Tonat. (2002). Hemorrhagic fever viruses as biological weapons: medical and public health management. JAMA 287:2391-2405
Drosten, C., S. Gottig, S. Schilling, M. Asper, M. Panning, H. Schmitz, and S. Gunther. (2002). Rapid detection and quantification of RNA of Ebola and Marburg viruses, Lassa virus, Crimean-Congo hemorrhagic fever virus, Rift Valley fever virus, dengue virus, and yellow fever virus by real-time reverse transcription-PCR. J. Clin. Microbiol. 40:2323-2330
Feldmann, H., S. Jones, H. D. Klenk, and H. J. Schnittler. (2003). Ebola virus: from discovery to vaccine. Nat. Rev. Immunol. 3:677-685
Ikegami, T., M. Niikura, M. Saijo, M. E. Miranda, A. B. Calaor, M. Hernandez, L. P. Acosta, D. L. Manalo, I. Kurane, Y. Yoshikawa, and S. Morikawa. (2003). Antigen capture enzyme-linked immunosorbent assay for specific detection of Reston Ebola virus nucleoprotein. Clin. Diagn. Lab. Immunol. 10:552-557
Leroy, E. M., S. Baize, C. Y. Lu, J. B. McCormick, A. J. Georges, M. C. Georges-Courbot, J. Lansoud-Soukate, and S. P. Fisher-Hoch. (2000). Diagnosis of Ebola haemorrhagic fever by RT-PCR in an epidemic setting. J. Med. Virol. 60:463-467.
Lucht, A., R. Grunow, P. Moller, H. Feldmann, and S. Becker. (2003). Development, characterization and use of monoclonal VP40-antibodies for the detection of Ebola virus. J. Virol. Methods 111:21-28.
Mitchell SW and McCormick JB, .J Clin. Microbiol. 1984, 20(3):486.)
Niedrig, M., H. Schmitz, S. Becker, S. Gunther, J. ter Meulen, H. Meyer, H. Ellerbrok, A. Nitsche, H. R. Gelderblom, and C. Drosten. (2004). First international quality assurance study on the rapid detection of viral agents of bioterrorism. J. Clin. Microbiol. 42:1753-1755.
Niikura, M., T. Ikegami, M. Saijo, I. Kurane, M. E. Miranda, and S. Morikawa. (2001). Detection of Ebola viral antigen by enzyme-linked immunosorbent assay using a novel monoclonal antibody to nucleoprotein. J. Clin. Microbiol. 39:3267-3271.
Mitchell S.W and McCormick J.B (1984), .J Clin. Microbiol. 1984, 20(3):486.)
Towner, J. S., P. E. Rollin, D. G. Bausch, A. Sanchez, S. M. Crary, M. Vincent, W. F. Lee, C. F. Spiropoulou, T. G. Ksiazek, M. Lukwiya, F. Kaducu, R. Downing, and S. T. Nichol. (2004). Rapid diagnosis of Ebola hemorrhagic fever by reverse transcription-PCR in an outbreak setting and assessment of patient viral load as a predictor of outcome. J. Virol. 78:4330-4341.
www.cdc.gov/HAI/pdfs/bbp/VHFinterimGuidance05_19_05.pdf, accessed August 11, 2014)