Effect of Huoxue Jiedu Jiangtang Formulation on Endoplasmic Reticulum Stress in Diabetic Atherosclerosis
American Journal of Clinical and Experimental Medicine
Volume 6, Issue 5, September 2018, Pages: 118-124
Received: Dec. 26, 2018;
Published: Dec. 27, 2018
Views 28 Downloads 13
Fu Xianzhao, Clinic Medical College of Youjiang Medical National College, Baise, China
Huang Wenhua, Clinic Medical College of Youjiang Medical National College, Baise, China
Li Chunyan, Clinic Medical College of Youjiang Medical National College, Baise, China
Li Xingchan, Clinic Medical College of Youjiang Medical National College, Baise, China
Qiu Haixian, Clinic Medical College of Youjiang Medical National College, Baise, China
Liang Liudan, Clinic Medical College of Youjiang Medical National College, Baise, China
Cao Qiuxia, Clinic Medical College of Youjiang Medical National College, Baise, China
Liang Liuyue, Clinic Medical College of Youjiang Medical National College, Baise, China
Objecive: To study the effects of Huoxue Jiedu Jiangtang formulation (HJJF) on endoplasmic reticulum stress (ERS) coupled inflammatory response From the molecular mechanism in diabetic vascular disease. Methods: Type 2 diabetes were established in male SD rats by feeding high lipid diet and injection of streptozotocin. after T2DM models established successfully, the rats were divided into model group, low-dose HJJF group (HJJF1), high-dose HJJF group (HJJF2), and Western medicine group (Gliquidone+Benazepril), and accepted corresponding drugs for 8 weeks respectively. Another normal group were used as control group, fed with normal diet, no drug intervention. The levels of fasting blood glucose (FBG), serum superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), IL-6 and TNF-α were tested. The ERS signaling molecules glucose-regulated protein 78 (GRP78) and c-Jun N-terminal kinases (JNK) mRNA transcription level in thoracic aorta vessel were determined by reverse-transcription polymerase chain reaction (RT-PCR). Results: Compared with model group, After drugs intervention, all administered groups could significantly decrease FPG (P<0.05), elevate SOD and GSH-Px (P<0.05); all could reduce levels of TC, TG, LDL-C, IL-6 and TNF-α (P<0.05), and raise HDL-C (P<0.05); all could depress the transcription levels of GRP78 and JNK mRNA (P<0.05). With increasing dose, the HJJF effect is more significant (P<0.05). Conclusion: HJJF could improve insulin resistance, correct lipid metabolism disorders, and enhance the antioxidative ability, inhibit the response of ERS coupled inflammatory, showing the multi-target treatment characteristics of Traditional Chinese Medicine.
Effect of Huoxue Jiedu Jiangtang Formulation on Endoplasmic Reticulum Stress in Diabetic Atherosclerosis, American Journal of Clinical and Experimental Medicine.
Vol. 6, No. 5,
2018, pp. 118-124.
Ozcan U, Cao Q, Yilmaz E, et al. Endoplasmic reticulum stress links obesity, insulin action, and type 2 Diabetes [J]. Science, 2004, 306 (5695): 457-461.
Wang M, Kaufman RJ. Protein misfolding in the endoplasmic reticulum as a conduit to human disease Nature, 2016, 529 (7586): 326-35.
Sanson M, Auge N, Vindis C, et al. Oxidized low-density lipoproteins trigger endoplasmic reticulum stress in vascular cells: prevention by oxygen-regulated protein 150 expression. Cire Res, 2009, 104: 328-336.
FU Xianzhao, XU Jing, HUANG Wenhua, et al. Strategy of Stasis-resolving and Detoxification on Diabetic Coronary Heart Disease [J]. JE TCM, 2014, 23 (11): 2024-2027.
FU Xian-zhao, XU Jing, LI Chun-yan, et al. Effects of Huoxue Jiedu Jiangtang formulation on the balance between pro- and anti-inflammatory cytokines in non-revascularization patients with diabetic acute coronary syndrome [J]. Chin JTCM WM Crit Care, 2016, 23 (1): 6-10.
Doultsinos D, Avril T, Lhomond S, et al. Control of the Unfolded Protein Response in Health and Disease. SLAS Discov, 2017, 22 (7): 787-800.
GUAN LiYing, XU CaiMin, PAN Hua Zhen. Endoplasmic Reticulam Stress-induced Apoptosis. Prigress in Biochemistry and Biophysics, 2007, 11: 1136-1141.
Hong F, Liu B, Wu BX, et al. CNPY2 is a key initiator of the PERK-CHOP pathway of the unfolded protein response. Nat Struct Mol Biol, 2017, 24 (10): 834-839.
Zhang K, Kaufman R J. From endoplasmic-retieulum stress to the inflammatory response. Nature, 2008, 454: 455-462
Lin JH, Walter P, Yen TS. Endoplasmic retieulum stress in disease pathogenesis. Annu Rev Pathol, 2008, 3: 399-425.
Tabas I, Seimon T, Timmins J, et al. Macrophage apoptosis in advanced atherosclerosis. Ann N Y Acad Sci. 2009, 1173 (Suppl 1): E40-45.
Lim W, Timmins J, Seimon T, et al. Signal transducer and activator of transcription-1 is critical for apoptosis in macrophages subjected to endoplasmic reticulum stress in vitro and in advanced atherosclerotic lesions in vivo [J]. Circulation, 2008. 117 (7): 940-951.
Lindholm D, Korhonen L, Eriksson O, et al. Recent Insights into the Role of Unfolded Protein Response in ER Stress in Health and Disease. Front Cell Dev Biol, 2017, 10: 5-48.
Zhang K, Kaufman RJ. From endoplasmic-reticulum stress to the inflammatory response [J]. Nature, 2008, 454: 455–462.
Luchetti F, Crinelli R, Cesarini E, et al. Endothelial cells, endoplasmic reticulum stress and oxysterols. Redox Biol, 2017, 13: 581-587.
Dickhou JG, Colgan SM, Lhotak S, et al. Increased endoplasmic reticulum stress in atherosclerotic plaques associated with acute coronary syndrome: A balancing act between plaque stability and rupture [J]. Circulation, 2007, 116 (11): 1226-1233.
Schmitz ML, Shaban MS, Albert BV, et al. The Crosstalk of Endoplasmic Reticulum (ER) Stress Pathways with NF-κB: Complex Mechanisms Relevant for Cancer, Inflammation and Infection [J]. Biomedicines, 2018, 6, 58; doi: 10.3390.
Fu Xianzhao, Huang Zhenfeng, Huang Wenhua, et al. Review and prospect of using traditional Chinese medicine drugs “supplem enting Qi and nourishin g Yin, activating blood circulation and detoxifying” for prevention and treatment of diabetes mellitus complicated with acute coronary syndrome
FU Xian-zhao, XU Jing, LI Chun-yan, et al. Effect of Huoxue Jiedu Jiangtang formulation on heart remodeling in diabetic patients with acute coronary syndrome after PCI [J]. Chin J TCM WM Crit Care, 2016, 23 (2): 117-121.