Profile of mRNA Expression of Ki-67 in Breast Cancer Patients Pre- and Post- Chemotherapy
American Journal of Clinical and Experimental Medicine
Volume 5, Issue 1, January 2017, Pages: 10-14
Received: Dec. 7, 2016;
Accepted: Dec. 20, 2016;
Published: Jan. 19, 2017
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Prihantono Prihantono, Department of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Daniel Sampepajung, Department of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Andi Asadul Islam, Department of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Mochammad Hatta, Molecular Biology and Immunology Laboratory, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Warsinggih Rahardjo, Department of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Christian Binekada, Department of Surgery, Faculty of Medicine, Haluoleo University, Kendari, Indonesia
Eddy Herman Tanggo, Department of Surgery, Faculty of Medicine, Airlangga University, Surabaya, Indonesia
Background: Proliferation is a distinct hallmarks of cancer. Ki-67 designated as a marker of proliferation in solid tumors. The proliferative activity of tumor demonstrated by expression of Ki-67 in breast cancer has been associated with a poor prognosis. Changes in the relative proportions of Ki-67 can be observed during chemotherapy and may correlated with clinical response in breast cancer. Purpose: Evaluate changes in mRNA expression of proliferation marker Ki-67 in breast cancer patients pre- and post-chemotherapy in relation with clinical response to chemotherapy. Method: This is a longitudinal study, 30 subjects breast cancer tissue samples pre- and post-chemotherapy using cyclophosphamide, adriamycin, 5FU regiment. We using qRT-PCR techniques to detect mRNA expression of Ki-67. Chemotherapy response is calculated using RECIST criteria. Results: Mean value of Ki-67 mRNA expression on breast cancer patients pre-chemotheraphy was 11.837±0.360. Mean value of Ki-67 mRNA expression on breast cancer patients post-chemotheraphy was 11.241±1.971. There was no significant correlation between expression of Ki-67 mRNA prechemotherapy with clinical response to chemotherapy, p = 0.862 (p ≥0.05). There is a positive correlation between velocity of Ki-67 mRNA expression with clinical response with value of r = 0.378, this correlation was significant with p = 0.020 (p<0.05). Conclusion: Chemotherapy cause decrease in mRNA expression of Ki-67. There is insignificant correlation between expression of mRNA Ki-67 baseline with chemotherapy response. Velocity of Ki-67 mRNA expression has significant correlation with clinical response to chemotherapy.
Andi Asadul Islam,
Eddy Herman Tanggo,
Profile of mRNA Expression of Ki-67 in Breast Cancer Patients Pre- and Post- Chemotherapy, American Journal of Clinical and Experimental Medicine.
Vol. 5, No. 1,
2017, pp. 10-14.
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