Effects of Preventive Long-Term Treatment with Strontium Ranelate and Zoledronic Acid on Bone Quality in Ovariectomized Rats
American Journal of Clinical and Experimental Medicine
Volume 4, Issue 6, November 2016, Pages: 191-200
Received: Sep. 3, 2016;
Accepted: Sep. 18, 2016;
Published: Nov. 15, 2016
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Marta Martín-Fernández, Biochemistry Investigation, Sanitary Research Institute Jimenez Diaz Foundation, Madrid, Spain
Marina Gómez-Chinchón, Biochemistry Investigation, Sanitary Research Institute Jimenez Diaz Foundation, Madrid, Spain
Luis Álvarez, Spinal Pathology Unit, Sanitary Research Institute Jimenez Diaz Foundation, Madrid, Spain
Blanca Torrubia, Biochemistry Investigation, Sanitary Research Institute Jimenez Diaz Foundation, Madrid, Spain
Manuel Díaz-Curiel, Internal Medicine, Sanitary Research Institute Jimenez Diaz Foundation, Madrid, Spain
David Guede, Trabeculae, Limited Society, Technological Park of Galicia, Ourense, Spain
Jose Ramón Caeiro, Traumatology and Orthopedic Surgery, Universitary Hospital of Santiago de Compostela, Santiago de Compostela, Spain
Cristobalina Rodríguez-Álvarez, Area of preventive Medicine and Public Health. Health Sciences Faculty, Medicine, University of La Laguna, Tenerife, Spain
Concepción de la Piedra, Biochemistry Investigation, Sanitary Research Institute Jimenez Diaz Foundation, Madrid, Spain
The aim of this work was to study the effects on bone quality produced by long-range treatment with zoledronic acid (ZA) or strontium ranelate (SrR) in ovariectomized rats. Sixty 6-month-old female Wistar rats were divided: SHAM (n=15), simulated intervention; OVX (n=15), ovariectomized; OVX+ZA (n=15), ovariectomized treated with ZA (0.083 mg/kg i. v. at the beginning of the study); and OVX+SrR (n=15), ovariectomized treated with SrR (0.033 g/kg/day by oral gavage). Rats were sacrificed 8 months later. Femoral (F) and lumbar (L) bone mineral density (BMD), trabecular and cortical microstructure, biomechanical testing and Raman spectrometry were performed. FBMD and LBMD decreased in OVX rats with no changes with SrR. ZA treatment prevented changes to BMD. Ovariectomy produced a reduction in micro-CT parameters, while SrR treatment did not avoid these changes. ZA treatment increased micro-CT parameters with respect to the SHAM group. The microstructural parameters of the cortical region were not modified in any of the groups. Ovariectomy produced a decrease in biomechanical parameters that was maintained with SrR. ZA treatment produced an increase in these parameters being higher than those of the SHAM group. Ovariectomy and treatments did not produce differences in crystallinity or substitution index. Mineralization index (MI) decreased in the OVX group. SrR did not revert this effect though ZA avoided it. According to our results preventive treatment with SrR did not revert the alterations in bone quality due to ovariectomy in rats. Treatment with ZA not only reverted the effects of ovariectomy, but also improved bone quality with respect to control rats.
Jose Ramón Caeiro,
Concepción de la Piedra,
Effects of Preventive Long-Term Treatment with Strontium Ranelate and Zoledronic Acid on Bone Quality in Ovariectomized Rats, American Journal of Clinical and Experimental Medicine.
Vol. 4, No. 6,
2016, pp. 191-200.
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