Evaluation of Anti-ulcerogenic Activity in Oil Extract of Jintan Hitam (Nigella sativa) Against Ethanol Induced Gastric Ulcer in Mice (Mus musculus)
American Journal of Clinical and Experimental Medicine
Volume 4, Issue 6, November 2016, Pages: 179-184
Received: Oct. 3, 2016; Accepted: Oct. 22, 2016; Published: Nov. 14, 2016
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Syamsu Rijal, Departement of Pathology Anatomy, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia; Departement of Histology, Faculty of Medicine, Universitas Muslim Indonesia, Makassar, Indonesia
Upik Anderiani Miskad, Departement of Pathology Anatomy, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia
Djumadi Achmad, Departement of Pathology Anatomy, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia
Rina Masadah, Departement of Pathology Anatomy, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia
Dasril Daud, Departement of Pediatric, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia
Cahyono Kaelan, Departement of Pathology Anatomy, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia
Halida Rahawarin, Departement of Pathology Anatomy, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia; Faculty of Medicine, Universitas Pattimura, Ambon, Indonesia
Swandari Paramita, Faculty of Medicine, Universitas Mulawarman, Samarinda, Indonesia
Yadi Yasir, Faculty of Medicine, Universitas Mulawarman, Samarinda, Indonesia
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Jintan hitam (Nigella sativa) has been widely used in traditional medicine to treat several diseases, including gastric ulcer. However, there are no scientific data demonstrating anti-ulcerogenic activity conferred by use of jintan hitam. The present study aimed to evaluate the antiulcer properties of oil extract from jintan hitam. In the gastric ulcer induced by administration of 80% ethanol model, observation made for ulcer score and index, level of ulcer erosion, total amount of polymorphonuclear cells and morphological change of 80% ethanol induced gastric ulcer of mice. Research was conducted on laboratory in Faculty of Medicine Universitas Hasanuddin from April to September 2015, with post test only experimental group design. There were 40 mice used in this research, divided into five groups, each group consisted of 8 mice: negative control group, positive control group, and three different doses of oil extract (0.1, 0.2 and 0.3 ml per 20g bw of mice). Observation made for 4 and 7 days. The oil extract of jintan hitam showed significant differences on doses of 0.1 ml per 20g bw of mice given in 4 days, with p = 0.034 (p<0.05) based on score and index of gastric ulcer.
Oil Extract of Jintan Hitam, Ulcer Score, Ulcer Index, Ulcer Erosion, Polymorphonuclear Cells, Morphological Change of Gastric
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Syamsu Rijal, Upik Anderiani Miskad, Djumadi Achmad, Rina Masadah, Dasril Daud, Cahyono Kaelan, Halida Rahawarin, Swandari Paramita, Yadi Yasir, Evaluation of Anti-ulcerogenic Activity in Oil Extract of Jintan Hitam (Nigella sativa) Against Ethanol Induced Gastric Ulcer in Mice (Mus musculus), American Journal of Clinical and Experimental Medicine. Vol. 4, No. 6, 2016, pp. 179-184. doi: 10.11648/j.ajcem.20160406.14
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