High Fat Diet Alters the Expression of M Cells and Claudin 4 in the Peyer’s Patches of Rats
American Journal of Clinical and Experimental Medicine
Volume 3, Issue 5, September 2015, Pages: 283-287
Received: Nov. 26, 2015;
Published: Nov. 26, 2015
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Auni Aqilah Zainal Abidin, Faculty of Medicine, Universiti Teknologi MARA (UiTM) Sungai Buloh Campus, Selangor, Malaysia
Effat Omar, Faculty of Medicine, Universiti Teknologi MARA (UiTM) Sungai Buloh Campus, Selangor, Malaysia
Mohammed Nasimul Islam, Faculty of Medicine, Universiti Teknologi MARA (UiTM) Sungai Buloh Campus, Selangor, Malaysia
Jesmine Khan, Faculty of Medicine, Universiti Teknologi MARA (UiTM) Sungai Buloh Campus, Selangor, Malaysia
Prevalence of obesity is increasing worldwide. One of the major risk factors for obesity is consumption of high fat diet (HFD). Gastrointestinal tract (GIT) is the first organ where HFD comes in contact with the body. But, the effect of HFD on the GIT especially the GIT barrier is not investigated properly. M cells are present in the Follicle associated epithelium (FAE) of Peyer’s patches (PP) of GIT and are important component of intestinal barrier. Healthy and adequate number of M cells is important for an effective intestinal barrier. Intestinal tight junction protein Claudin 4, situated in between the enterocytes of Peyer’s patches (PP), regulates the permeability through the intestinal mucosa. Reduced Claudin 4 is responsible for increased paracellular transport of antigenic materials. Calprotectin is an inflammatory marker secreted by neutrophils during inflammation. Its level is considered specific for intestinal inflammation. The objectives of this study were to investigate the expressions of M cells and Claudin 4 in the PP and to determine the fecal calprotectin level (FCP) of male Wistar rats fed HFD. Four weeks old, twenty male Wistar rats were divided into chow (n=10) and HFD (n=10) groups. After 6 weeks of consuming the respective diets, stool and GIT segments containing PP were collected. After tissue processing, tissues were sectioned into 3 micrometer thickness and were taken on poly-L-lysine coated glass slides. Immunohistochemical staining was done by rat M cell specific CK-8 antibody and anti-Claudin 4 antibody. Scoring was done to calculate the average number of M cells and Claudin 4 in the PP of both groups under light microscope. FCP were measured using a commercial enzyme linked immunoassay (ELISA) kit. Statistical analysis was done by chi-square test and independent T-test. Data are presented as mean ± SD. A p value <0.05 was considered as significant. The number of M cells in the PP was significantly higher in HFD group as compared to the control (p = 0.004). The expression of Claudin 4 in the PP was significantly decreased in HFD group as compared to the control (p = 0.018). The fecal calprotectin level in HFD group was significantly higher compared to the control (p = 0.016). HFD consumption for 6 weeks leads to a higher number of M cell and reduced the expression of Claudin 4 in the intestinal Peyer’s patches of male Wistar rats which might be due to GI inflammation.
Auni Aqilah Zainal Abidin,
Mohammed Nasimul Islam,
High Fat Diet Alters the Expression of M Cells and Claudin 4 in the Peyer’s Patches of Rats, American Journal of Clinical and Experimental Medicine.
Vol. 3, No. 5,
2015, pp. 283-287.
Rindler PM, Plafker SM, Szweda LI, Kinter M. High dietary fat selectively increases catalase expression within cardiac mitochondria. J Biol Chem. 2012 Nov 30. [Epub ahead of print]
Ge, J., Wang, J., Xue, D., Zhu, Z., Chen, Z., Li, X., Su, D., and Du, J. (2013). Why does a high-fat diet induce preeclampsia-like symptoms in pregnant rats. Neural Regen. Res. 8, 1872–1880.
Namavar MR, Raminfard S, Jahromi ZV, Azari H. Effects of high-fat diet on the numerical density and number of neuronal cells and the volume of the mouse hypothalamus: a stereological study. Anat Cell Biol. 2012 Sep; 45(3): 178-84. doi: 10.5115/acb.2012.45.3.178. Epub 2012 Sep 30.
Luzzo KM, Wang Q, Purcell SH, Chi M, Jimenez PT, Grindler N, Schedl T, Moley KH. High fat diet induced developmental defects in the mouse: oocyte meiotic aneuploidy and fetal growth retardation/brain defects. PLoSOne. 2012; 7(11): e49217. doi: 10.1371/journal.pone.0049217. Epub 2012 Nov 12.
Miller, H., Zhang, J., KuoLee, R., Patel, G.B., and Chen, W. (2007b). Intestinal M cells: The fallible sentinels? World J. Gastroenterol. WJG 13, 1477–1486.
Franke, W.W. (2009). Discovering the Molecular Components of Intercellular Junctions—A Historical View. Cold Spring Harb. Perspect. Biol. 1.
Zeissig, S., Bürgel, N., Günzel, D., Richter, J., Mankertz, J., Wahnschaffe, U., Kroesen, A.J., Zeitz, M., Fromm, M., and Schulzke, J. (2007). Changes in expression and distribution of claudin 2, 5 and 8 lead to discontinuous tight junctions and barrier dysfunction in active Crohn’s disease. Gut 56, 61–72.
Bischoff, S.C., Barbara, G., Buurman, W., Ockhuizen, T., Schulzke, J.-D., Serino, M., Tilg, H., Watson, A., and Wells, J.M. (2014). Intestinal permeability – a new target for disease prevention and therapy. BMC Gastroenterol. 14.
Tamagawa H, Takahashi I, Furuse M, Yoshitake-Kitano Y, Tsukita S, Ito T, Matsuda H, Kiyono H. Characteristics of claudin expression in follicle-associated epithelium of Peyer's patches: preferential localization of claudin-4 at the apex of the dome region. Lab Invest. 2003 Jul; 83(7): 1045-53.
Erbayrak, M., Turkay, C., Eraslan, E., Cetinkaya, H., Kasapoglu, B., and Bektas, M. (2009). The role of fecal calprotectin in investigating inflammatory bowel diseases. Clin. São Paulo Braz. 64, 421–425.
Dixon et al (1996). Classification and grading of gastritis. The updated Sydney System. International Workshop on the Histopathology of Gastritis, Houston 1994.
Jesmine Khan, Mariam Mohamad, Gabriele Ruth Anisah Froemming, Mohammed Nasimul Islam. High Fat Diet Increases Uptake of Particles by the Peyer's Patches of Ileum in Rats IMJ 20(4,) 232-234, 2013.
Ouzilou, L., Caliot, E., Pelletier, I., Prévost, M.-C., Pringault, E., and Colbère-Garapin, F. (2002). Poliovirus transcytosis through M-like cells. J. Gen. Virol. 83, 2177–2182.
de La Serre CB, Ellis CL, Lee J, Hartman AL, Rutledge JC, Raybould HE. Propensity to high-fat diet-induced obesity in rats is associated with changes in the gut microbiota and gut inflammation. Am J Physiol Gastrointest Liver Physiol. 2010 Aug; 299(2): G440-8. doi: 10.1152/ajpgi.00098.2010. Epub 2010 May 27.
Kucharzik T, Lügering A, Lügering N, Rautenberg K, Linnepe M, Cichon C, Reichelt R, Stoll R, Schmidt MA, Domschke W. Characterization of M cell development during indomethacin-induced ileitis in rats. Aliment PharmacolTher 2000, 14, 247-56.
Y Lee, C. (2013). The Effect of High-Fat Diet-Induced Pathophysiological Changes in the Gut on Obesity: What Should be the Ideal Treatment? Clin. Transl. Gastroenterol. 4, e39.