Antimicrobial Activity of Lippia adoensis var. koseret Against Human Pathogenic Bacteria and Fungi
American Journal of Clinical and Experimental Medicine
Volume 3, Issue 3, May 2015, Pages: 118-123
Received: Mar. 29, 2015; Accepted: Apr. 12, 2015; Published: May 19, 2015
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Authors
Gemechu Ameya Buli, Department of Medical Laboratory Science, College of Medicine and Health Sciences, Arba Minch University, Arba Minch, Ethiopia
Abdella Gure Duga, Wondo Genet Colleges of Forestry and Natural Resources, Hawassa University, Hawassa, Ethiopia
Engda Dessalegn, Department of Chemistry, Hawassa College of Education, Hawassa, Ethiopia
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Abstract
Background: Lippia adoensis var. koseret is a well known medicinal plant endemic to Ethiopia. It has been traditionally used to treat different infectious diseases and also in food preparation as condiment. The aim of the current study was to evaluate antibacterial and antifungal activities of water, ethanol and methanol based crude extracts of L. adoensis var. koseret against selected human pathogenic bacteria and fungi. Methods: Crude extracts of L. adoensis var. koseret were extracted by maceration method. Disc diffusion assay of the extracts were carried out in four different concentrations against three different bacteria species (Staphylococcus aureus, Enterococcus faecalis and Escherichia coli) and two clinical isolated fungal species (Candida albicans and Aspergillus flavus) by using Kirby- Baur disk diffusion method. Agar dilution method was used to determine the minimum inhibitory concentration, the minimum bactericidal and fungicidal concentrations of the extracts against similar microorganisms. Results: Water-based extract of L. adoensis var. koseret exhibited significantly less antimicrobial activity as compared to ethanol and methanol crude extract against tested isolates of bacteria and fungi (P < 0.05); while, there was no significant difference between ethanol and methanol extracts. Among the tested microorganism S. aureus, was the most sensitive of all whereas C. albicans was the most resistant microorganism to alcohol based extract of L. adoensis var. koseret. The minimum inhibitory concentration of L. adoensis var. koseret ranged from 3.12 to 25mg/ml in the alcohol based extracts but it was higher in the water-based extract. The lower bactericidal concentration (5.20 mg/l) and the highest fungicidal concentration (37.50 mg/ml) were observed in methanol based extracts against S. aureus and C. albicans, respectively. Conclusions: Antimicrobial activity of L. adoensis var. koseret varies with extraction solvent and tested microorganisms.
Keywords
Antibacterial Activity, Antifungal Activity, Crude Extract, L. adoensis var. koseret
To cite this article
Gemechu Ameya Buli, Abdella Gure Duga, Engda Dessalegn, Antimicrobial Activity of Lippia adoensis var. koseret Against Human Pathogenic Bacteria and Fungi, American Journal of Clinical and Experimental Medicine. Vol. 3, No. 3, 2015, pp. 118-123. doi: 10.11648/j.ajcem.20150303.18
References
[1]
Yogayata SP, Vijay DW (2012). Herbal medicines and nutritional supplements used in the treatment of glaucoma. Research Journal of Pharmaceutical, Biological and Chemical Sciences 3 (1): 331
[2]
Fabricant DS, Farnsworth NR. (2001). The value of plants used in traditional medicine for drug discovery. Environ Health Perspect 109: 69-75.
[3]
Tapsell LC, Hemphill I, Cobiac L, Patch CS, Sullivan DR, Fenech M, et al. (2006). Health benefits of herbs and spices: the past, the present, the future. Med J Aust 4:185.
[4]
Abebe D, Ayehu A. (1993). Medicinal plants and Engymatic Health practices of Northern Ethiopia, Addis Ababa. Berhanena Selam Printing Press Pp. 219-221
[5]
Nigist A, Sebsebe D. (2009). Aromatic Plants of Ethiopia, Shama books, Addis ababa, Ethiopia. Pp. 137–138.
[6]
Riot S, Mariamawit Y, Ameha S, Kaleab A. (2005). Radical scavenging activity of volatile oils of herbs traditionally used to spice cooking butter in Ethiopia. Ethiopian Pharmaceutical Journal 23: 7-14
[7]
Hailu T, Endris M, Kaleab A, Tsige G. (2005). Antimicrobial activities of some selected traditional Ethiopian medicinal plants used in the treatment of skin disorders. Journal of Ethnopharmacology 10: 168-175
[8]
Megersa M, Zemede A, Ensermu K, Abebe B, Bizuneh W. (2013). An ethnobotanical study of medicinal plants in Wayu Tuka District, East Welega Zone of Oromia Regional State, West Ethiopia. Journal of Ethnobiology and Ethnomedicine 9: 184-192.
[9]
Abegaz B, Asfaw N, Lwande W. (1993). Constituents of Essential Oils from Wild and Cultivated Lippia adoensis. J Essent Oil Res 5: 487-491
[10]
Berhanu A, Nigist A, Wilber L. (2001). Constituents of the Essential Oils from Wild and Cultivated Lippia adoensis Hochst. ex Walp. Journal of Essential Oil Research 5: 487–491
[11]
World Health Organization. (1996). A new global health crisis: emerging infectious diseases. World Health Report. Geneva
[12]
Parekh J, Nair R, Chanda S. (2005). Preliminary screening of some folkloric plants from Western India for potential antimicrobial activity. Indian J. Pharmacol 37: 408-409
[13]
Handa SS, Khanuja SPS, Longo G, Rakesh DD. (2008). Extraction technologies for medicinal and aromatic plants. Trieste: ICS UNIDO
[14]
Clinical and Laboratory Standards Institute. (2009). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically approved standard. vol. 29, 18th edn. M 07-A8. Wayne, Pa, USA: CLSI
[15]
Yared W, Tesfalem A, Solomon A. (2014). Evaluation of Antibacterial Activity and Phytochemical Constituents of Leaf Extract of Lippia adoensis. Asia Pacific Journal of Energy and Environment 1:1
[16]
Bimakr M (2010). Comparison of different extraction methods for the extraction of major bioactive flavonoid compounds from spearmint (Mentha spicata L.) leaves. Food Bioprod Process 1: 6
[17]
Lapornik B, Prosek M, Wondra AG. (2005). Comparison of extracts prepared from plant by-products using different solvents and extraction time. Journal of Food Engineering, 71: 214-22
[18]
Wang GX. (2010). In vivo anthelmintic activity of five alkaloids from Macleaya microcarpa (Maxim) Fedde against Dactylogyrus intermedius in Carassius auratus. Veterinary Parasitology 171: 305-13
[19]
Cowan MM. (1999). Plant products as antimicrobial agents. Clinical microbiology reviews 12 (Suppl 4): 564-82
[20]
Vlietinck AJ, Vanhoof L, Totte J, Lasure A, Vanden BD, Rwangabo PC, et al:(1995). Screening of hundred Rwandese medicinal plants for antimicrobial and antiviral properties Ethnopharmacol, 46: 31-47
[21]
Hsieh P, Siegel S, Rogers B, Lewis K. (1998). Bacteria lacking a multidrug pump: a sensitive tool for drug discovery. Proc Natl Acad Sci, 95: 6602-6
[22]
Kumar A, Khan IA, Koul S. (2008). Novel structural analogues of piperine as inhibitors of the NorA efflux pump of S. aureus. J Antimicrob Chemother, 61:1270-6
[23]
Cherigo L, Pereda-Miranda R, Fragoso-Serrano M, Jacobo-Herrera N, Kaatz GW, Gibbons S. (2008). Inhibitors of bacterial multidrug efflux pumps from the resin glycosides of Ipomoea murucoides. J Nat Prod, 71:1037-45
[24]
Schultes RE. (1978). The kingdom of plants, In W. A. R. Thomson (ed.), Medicines from the Earth. McGraw-Hill Book Co., New York
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