Multiple Sclerosis: Effect of Oral Administration of an Antioxidant Dietary Supplement in C57BL6/N Induced Model of Experimental Autoimmune Encephalomyelitis
American Journal of Clinical and Experimental Medicine
Volume 3, Issue 3, May 2015, Pages: 83-87
Received: Mar. 26, 2015;
Accepted: Apr. 9, 2015;
Published: Apr. 18, 2015
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Torricelli Piera, Department SPES, University of Molise, Campobasso, Italy
Antonelli Francesco, University of Tor Vergata, Department of Biology, Rome, Italy
Ferorelli Pasquale, University of Tor Vergata, Department of Biology, Rome, Italy
De Martino Angelo, University of Tor Vergata, Department of Biology, Rome, Italy
Shevchenko Anna, People’s Friendship University of Russia, Department of medicine, Moscow, Russia
Beninati Simone, University of Tor Vergata, Department of Biology, Rome, Italy
In the pathogenesis of demyelinating diseases including multiple sclerosis (MS) an important role is played by oxidative stress. Increased energy requirements during remyelination of axons and mitochondria failure is one of the causes of axonal degeneration and disability in MS. In the presence of neurological diseases such as MS, F2-isoprostanes are concentrated in higher quantities. In this context, we analyzed the levels of F2-isoprostanes in the plasma and cerebrospinal fluid of mice of the strain C57BL6/N with an induced experimental autoimmune encephalomyelitis (EAE), orally treated with two concentrations of Citozym, a dietary supplement with evident antioxidant properties. Compared to the control group, Citozym-treated EAE-mouse had significantly lower levels of F2-isoprostanes both in plasma and in cerebrospinal fluid. Furthermore, according to the guidelines of IACUC, treatment with Citozym at higher concentrations drastically reduced neurological signs of induced EAE.
De Martino Angelo,
Multiple Sclerosis: Effect of Oral Administration of an Antioxidant Dietary Supplement in C57BL6/N Induced Model of Experimental Autoimmune Encephalomyelitis, American Journal of Clinical and Experimental Medicine.
Vol. 3, No. 3,
2015, pp. 83-87.
B. Uttara, A. V. Singh, P. Zamboni, and R. T. Mahajan, “Oxidative stress and neurodegenerative diseases: a review of upstream and downstream antioxidant therapeutic options. Current Neuropharmacology, 2009; 7(1): 65–74
R. Dutta and B. D. Trapp, “Pathogenesis of axonal and neuronal damage in multiple sclerosis. Neurology. 2007;68 (22): S22–S31.
H. S. Mickel, “Multiple sclerosis: a newhypothesis. Perspectives in Biology and Medicine. 1975; 18 (3): 363–374.
H. T. Besler and S. Comoglu. Lipoprotein oxidation, plasma total antioxidant capacity and homocysteine level in patients with multiple sclerosis. Nutritional Neuroscience. 2003; 6 (3):189–196.
J. A. Black, J. Newcombe, B. D. Trapp, and S. G. Waxman, Sodium channel expression within chronic multiple sclerosis Plaques. Journal of Neuropathology and Experimental Neurology. 2007; 66 (9): 828–837.
B. D. Trapp, and S. G. Waxman, Sodium channel expression within chronic multiple sclerosis Plaques. Journal of Neuropathology and Experimental Neurology. 2007; 66 (9): 828–837.
A. Ames III. Central nervous system energy metabolism as related to function. Brain Research Reviews. 2000; 34 (1-2): 42–68.
H. Andrews, K. White, C. Thomson et al. Increased axonal mitochondrial activity as an adaptation to myelin deficiency in the shiverer mouse. Journal of Neuroscience Research. 2006; 83 (8): 1533–1539.
E. Karg, P. Kliv´enyi, I. N´emeth, K. Bencsik, S. Pint´er, and L. V´ecsei. Nonenzymatic antioxidants of blood in multiple sclerosis. Journal of Neurology. 1999; 246 (7):533–539.
GL. Milne, Q. Dai, LJ. Roberts LJ 2nd. The isoprostanes-25 years later. Biochim Biophys Acta. 2015; 1851(4):433-445.
R.Gold, C. Linington, H. Lassmann. Understanding pathogenesis and therapy of multiple sclerosis via animal models: 70 years of merits and culprits in experimental autoimmune encephalomyelitis research. Brain. 2006 (Pt 8):1953-1971.
K. O'Brien, D. Fitzgerald, A.Rostami, B.Gran. The TLR7 agonist, imiquimod, increases IFN-beta production and reduces the severity of experimental autoimmune encephalomyelitis. J Neuroimmunol. 2010;221(1-2):107-111
GL. Milne, H. Yin, JD. Brooks, S. Sanchez, L. Jackson Roberts 2nd, JD. Morrow JD. Quantification of F2-isoprostanes in biological fluids and tissues as a measure of oxidant stress. Methods Enzymol. 2007;433:113-126.
DH. Mahad, BD. Trapp, H. Lassmann H. Pathological mechanisms in progressive multiple sclerosis. Lancet Neurol. 2015;14(2):183-193
A. Langer-Gould, L. Qian, SY. Tartof, SM. Brara, SJ. Jacobsen, BE. Beaber, LS. Sy, C. Chao, R. Hechter, HF. Tseng. Vaccines and the risk of multiple sclerosis and other central nervous system demyelinating diseases. JAMA Neurol. 2014; 71(12):1506-1513
D. Ontaneda, RJ. Fox, J. Chataway. Clinical trials in progressive multiple sclerosis: lessons learned and future perspectives. Lancet Neurol. 2015; 14(2):208-223.
VK. Harris, SA. Sadiq. Biomarkers of therapeutic response in multiple sclerosis: current status. Mol Diagn Ther. 2014; 18(6):605-617.
G.G. Ortiz, F. P. Pacheco-Moisés, O. K. Bitzer-Quintero, A. C. Ramírez-Anguiano, L. J. Flores-Alvarado, V. Ramírez -Ramírez, M. A. Macias-Islas, E. D. Torres-Sánchez. Immunology and Oxidative Stress in Multiple Sclerosis. Clinical and Basic Approach. Neuroscience Letters. 2007; 414(3):233-236.
P. Torricelli, P. Ferorelli, A. De Martino, F. Antonelli, A. Shevchenko, S. Beninati. Regression of Carotid Plaques in Individuals at Low-to-intermediate Cardiovascular Risk Treated with Citozym and Propulzym. European Journal of Preventive Medicine. 2014; 2 (3): 33-37.
P. Torricelli, P. Ferorelli, A. De Martino, F. Antonelli, S.Beninati. Preventive Effects of A Mixture of Micronutrients with antioxidative Properties on Experimentally Induced Prostate Hyperplasia. American J Life Sci. 2013; 1(1): 22-26.
P. Torricelli, F. Antonelli , P. Ferorelli , A. De Martino , A. Shevchenko , S. Beninati. Antiproliferative Activity of a Dietary supplement on Estrogen Receptor Positive and Negative Human Breast Adenocarcinoma Cell Lines. Cancer Research Journal. 2014; 2 (2), 2014:29-32.