Analysis of Oxidative DNA Damage / Oxidative Stress Markers in Patients with Ovarian Cancer
American Journal of Clinical and Experimental Medicine
Volume 1, Issue 2, September 2013, Pages: 40-43
Received: Aug. 21, 2013; Published: Sep. 20, 2013
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Krzysztof Roszkowski, Department of Radiotherapy, the F. Lukaszczyk Oncology Center, Bydgoszcz, Poland
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Although the role of oxidative stress in the process of carcinogenesis seems well known, the quantitative correlation between oxidative DNA damage and the degree of histological malignancy of a neoplasm has not yet been determined. In the present study we attempted to show the possible correlations by looking at the amounts of the basic oxidative stress markers 8-oxoGua (8-oxo-7.8-dihydroguanine) and 8-oxodG (8-oxo-7.8-dihydro-2’-deoxyguanosine) excreted in the urine of patients. All the previously mentioned modifications were analyzed using techniques involving high performance liquid chromatography/electrochemical detection (HPLC/EC) or HPLC/gas chromatography and mass spectroscopy (GC-MS). All patients (n=46) suffered from ovarian cancer and were divided into three groups: G1, G2, and G3, according to the degree of histological malignancy of the neoplasm. In the female patients whose neoplasms showed higher degrees of histological malignancy significantly higher median values of 8-oxoGua and 8-oxodG were found to be excreted in their urine. In the subgroup of patients with G3 feature, both these markers of oxidative stress were almost twice as high as in the subgroup with G1 feature. The results suggest that the oxidative stress in ovarian cancer patients as demonstrated by increased amounts of these modifications in urine may be typical not only for the affected tissue, but also for other tissues and even the whole organism. It is also possible that the accumulation of oxidative stress is proportional to the degree of histological malignancy and depends on histological diagnosis.
Ovarian Cancer, Oxidative Stress, Oxidative DNA Damage, Histological Diagnosis
To cite this article
Krzysztof Roszkowski, Analysis of Oxidative DNA Damage / Oxidative Stress Markers in Patients with Ovarian Cancer, American Journal of Clinical and Experimental Medicine. Vol. 1, No. 2, 2013, pp. 40-43. doi: 10.11648/j.ajcem.20130102.12
Olinski R, Gackowski D, Foksinski M, Rozalski R, Roszkowski K, Jaruga P. Oxidative DNA damage: assessment of the role in carcinogenesis, atherosclerosis and acquired immunodeficiency syndrome. Free Rad Biol Med 2002; 33:192-200.
Tudek B, Speina E. Oxidatively damaged DNA and itsrepair in colon carcinogenesis. Mutat Res2012; 736: 82-92.
Tudek B, Winczura A, Janik J, Siomek A, Foksinski M, Olinski R. Involvement of oxidatively damaged DNA and repair in cancer development and aging. Am J Transl Res 2010; 2: 254-284.
Cooke MS, Olinski R, Loft S. Measurement and Meaning of Oxidatively Modified DNA Lesions in Urine. Cancer Epidemiol Biomarkers Prev 2008; 17(1): 3-14.
Wiseman H, Kaur H, Halliwell B. DNA damage and cancer: measurement and mechanism. Cancer Lett 1995; 93: 113-120.
Cooke MS, Olinski R, Evans MD. Does measurement of oxidative damage to DNA have clinical significance? ClinChimActa2006; 365: 30-49.
Leitch EF, Chakrabarti M, Crozier JE, McKee RF, Anderson JH, Horgan PG, McMillan DC. Comparison of the prognostic value of selected markers of the systemic inflammatory response in patients with colorectal cancer. Br J Cancer2007; 97: 1266-1270.
Roszkowski K, Olinski R. Urinary 8-Oxoguanine as a Predictor of Survival in Patients Undergoing Radiotherapy. Cancer Epidemiol Biomarkers Prev 2012; 21: 629-634.
Szekeres-Bartho J, Polgar B. PIBF: the double edged sword. Pregnancy and Tumor. Am J ReprodImmunol 2010; 64: 77-86.
Mor G, Yin G, Chefetz I, Yang Y, Alvero A. Ovarian cancer stem cells and inflammation. Cancer Biology & Therapy 2011; 11(8): 708-713.
Roszkowski K, Jozwicki W, Blaszczyk P, Mucha-Malecka A, Siomek A. Oxidative damage DNA: 8-oxoGua and 8-oxodG as molecular markers of cancer. Med SciMonit 2011; 17(6): 329-333.
Karihtala P, Soini Y, Vaskivuo L, Bloigu R, Puistola U. DNA adduct 8-hydroxydeoxyguanosine, a novel putative marker of prognostic significance in ovarian carcinoma. Int J GynecolCancer 2009; 19(6): 1047-1051.
Gackowski D, Rozalski R, Roszkowski K, Jawien A, Foksinski M, Olinski R. 8-Oxo-7,8-dihydroguanine and 8-oxo-7,8-dihydro-20-deoxyguanosine levels in human urine do not depend on diet. Free Radic Res 2001; 35: 825–832.
Rozalski R, Gackowski D, Roszkowski K, Foksinski M, Olinski R. The level of 8-hydroxyguanine, a possible repair product of oxidative DNA damage, is higher in the urine of cancer patients than in control subjects. Cancer Epidemiol Biomarkers Prev 2002; 11: 1072-1075.
Cooke MS, Rozalski R, Dove R, Gackowski D, Siomek A, Evans MD, Oliński R. Evidence for attenuated cellular 8-oxo-7,8-dihydro-29-deoxyguanosine removal in cancer patients. BiolChem 2006; 387(4): 393-400.
Roszkowski K, Gackowski D, Rozalski R, Dziaman T, Siomek A, Guz J, Szpila A, Foksinski M and Olinski R. Small field radiotherapy of head and neck cancer patients is responsible for oxidatively damaged DNA/oxidative stress on the level of a whole organism. Int J Cancer 2008; 123: 1964–1967.
Sonoda K, Miyamoto S, Yotsumoto F, Yagi H, Nakashima M, Wtanabe T, Nakano H. Clinical significance of RCAS1 as a biomarker of ovarian cancer. Oncology Reports 2007; 17: 623-628.
Siomek A, Tujakowski J, Gackowski D, Rozalski R, Foksinski M, Dziaman T, Roszkowski K and Olinski R. Severe oxidatively damaged DNA after cisplatin treatment of cancer patients. Int J Cancer 2006; 119: 2228–2230.
Chiou CC, Chang PY, Chan EC, Wu TL, Tsao KC, Wu JT. Urinary 8-hydroxydeoxyguanosine and its analogs as DNA marker of oxidative stress: development of an ELISA and measurement in both bladder and prostate cancers. ClinChimActa 2003; 334(1-2): 87-94.
Loft S, Svoboda P, Kawai K, Kasai H, Sørensen M, Tjønneland A, Vogel U, Møller P, Overvad K, Raaschou-Nielsen O. Association between 8-oxo-7,8-dihydroguanine excretion and risk of lung cancer in a prospective study. Free RadicBiol Med 2012; 52(1): 167-172.
Postawski K, Przadka-Rabaniuk D, Piersiak T. 8-oxo-7,8-dihydroguanine level - the DNA oxidative stress marker - recognized by fluorescence image analysis in sporadic uterine adenocarcinomas in women. Ginekol Pol 2013; 84(1): 44-50.
Maynard S, Schurman SH, Harboe C, de Souza-Pinto NC, Bohr VA. Base excision repair of oxidative DNA damage and association with cancer and aging. Carcinogenesis 2009; 30(1): 2-10.
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