American Journal of Internal Medicine
Volume 5, Issue 1, January 2017, Pages: 1-6
Received: Nov. 15, 2016;
Accepted: Nov. 30, 2016;
Published: Jan. 3, 2017
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Maha Assem, Internal Medicine Department, Faculty of Medicine, Cairo University, Cairo, Egypt
Mohamed-Naguib Abdalla Wifi, Internal Medicine Department, Faculty of Medicine, Cairo University, Cairo, Egypt
Rasha Elsherif, Clinical and Chemical Pathology Department, Faculty of Medicine, Cairo University, Cairo, Egypt
Ahmed Saad, Internal Medicine Department, Faculty of Medicine, Cairo University, Cairo, Egypt
Dalia Kadry Ismail, Clinical and Chemical Pathology Department, Faculty of Medicine, Cairo University, Cairo, Egypt
Ahmed Hasanin, Anesthesiology Department, Faculty of Medicine, Cairo University, Cairo, Egypt
Rasha Bassyouni, Medical Microbiology and Immunology Department, Faculty of Medicine, Fayoum University, Fayoum, Egypt
Mohamed Saeed Hussein Gomaa, Internal Medicine Department, Faculty of Medicine, Cairo University, Cairo, Egypt
Multidrug-resistant Gram-negative organisms have emerged as a major threat to hospitalized patients, and are associated with serious morbidity and mortality. This study aimed to characterize carbapenem resistance genes among Gram-negative bacilli isolated from clinical samples from patients in the intensive care unit of Cairo University Hospital. A total of 211 samples were collected from patients showing clinical evidence of infection. Bacteria were isolated and identified by conventional microbiological methods. Acinetobacter baumannii isolates were furtherly characterized by polymerase chain reaction (PCR), using primers specific for blaOXA-51-like genes. The Kirby Bauer disc diffusion method was used to determine susceptibility patterns of isolates, and carbapenem resistance was further examined by a modified Hodge test. Positive isolates were tested for the presence of blaKPC, blaOXA-48, and blaNDM-like genes by PCR. NDM gene types were determined by direct sequencing. From the 211 samples, 229 Gram-negative bacilli were isolated. Fifty isolates (21.2%) were resistant to carbapenem. PCR analysis showed that none of the 50 isolates carried blaKPC-like genes, while 24 (48%) isolates carried blaOXA-48-like genes, 8 (16%) carried blaNDM-1, and five isolates (10%) carried both blaNDM-1 and blaOXA-48-like genes. These results indicate that continuous surveillance of these multidrug-resistant pathogens is urgently required. And that is very important is to activate the antimicrobial stewardship programs of which the most important is restriction of the big gun antibiotics like carbapenems, colistin, tigecyclin and vancomycin and restricting their prescription to privileged specialties.
Mohamed-Naguib Abdalla Wifi,
Dalia Kadry Ismail,
Mohamed Saeed Hussein Gomaa,
Emergence of Gram-Negative Bacilli with Concomitant blaNDM-1- and blaOXA-48-Like Genes in Egypt, American Journal of Internal Medicine.
Vol. 5, No. 1,
2017, pp. 1-6.
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