Effect of Superfine Grinding on the Phytochemicals and Antioxidant Activities of Mulberry Leaves
Science Journal of Public Health
Volume 4, Issue 3, May 2016, Pages: 138-146
Received: Mar. 9, 2016;
Accepted: Mar. 18, 2016;
Published: Apr. 12, 2016
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Edwin H. Mena, Food Science, School of Life Sciences, Shanghai University, Shanghai, China
Tao Liu, Food Science, School of Life Sciences, Shanghai University, Shanghai, China
Xianyan Liao, Food Science, School of Life Sciences, Shanghai University, Shanghai, China
Tosin M. Olajide, Food Science, School of Life Sciences, Shanghai University, Shanghai, China
Junyi Huang, Food Science, School of Life Sciences, Shanghai University, Shanghai, China
In this study, two species of mulberry leaves were used as materials and four different size powders (about 20-115 µm, 15-20 µm, 10-15 µm and <10 µm) were prepared. The effects of superfine grinding (SG) on the extraction of active components from mulberry leaves (MLs) and their antioxidant activities were investigated. The results indicated that the contents of the active component such as polyphenol, polysaccharides, alkaloids were increased markedly with the decreasing in the particle size, but there was a bit difference in flavonoids. Additionally, the antioxidants properties of MLs treated on 1,1-diphel-2-picrylhydraxyl (DPPH) radical scavenging activity and ferric reducing antioxidant power (FRAP) were also increased, but the scavenging activity in hydroxyl radical scavenging assay (HAS) decreased in the size of D10. These results indicated that SG could improve the extraction efficiency of active components from MLs and make its antioxidant activity enhance, implying that SG can be an effective way to improve the quality and functionality of MLs in foodstuff and pharmacological field.
Edwin H. Mena,
Tosin M. Olajide,
Effect of Superfine Grinding on the Phytochemicals and Antioxidant Activities of Mulberry Leaves, Science Journal of Public Health.
Vol. 4, No. 3,
2016, pp. 138-146.
Yang X, Yang L and Zheng H. Hypolipidemic and antioxidant effects of mulberry (Morusalba L.) fruit in hyperlipidaemia rats. Food Chem, 2010, 48(8-9): 2374-2379.
Ercisli S and Orhan E. Chemical composition of white (Morusalba), red (Morusrubra) and black (Morusnigra) mulberry fruits. Food Chem, 2007, 103(4): 1380-1384.
Kang T. H, Hur J. Y, Kim H. B, Ryu J. H. and Kim S. Y. Neuroprotective effects of the cyaniding- 3-O-beta-glucopyranoside isolated from mulberry fruit against cerebralischemia. Neurosci. Lett, 2006, 391(3): 168-172.
Chen PN, Chu S. C, Chiou, Kuo W. H and Chiang C. L. Mulberry anthocyanins, cyaniding 3-rutinoside and cyanidin 3-glucoside, exhibited and inhibitory effect on the migration and invasion of a human lung cancer cell line. Hsieh, 2006, 235(2): 248-259.
Liu L. K, Lee H. J, Shih YW, Chyau C. C and Wang C. J. Mulberry anthocyanin extracts inhibit LDL oxidation and macrophage-derived foam cell formation induced by oxidation LDL. Food Sci, 2008, 73(73): 113-121.
Chang L. W, Juang L. J, Wang B. S, Wang M. Y, Tai H. M, Huang W. J and Chen Y. J. Antioxidant and antityrosinase activity of mulberry (Morusalba L.) twigs and root bark. Food Chem, 2011, 49(4): 778-790.
Kim Y, Kim M, Takaya Y, Niwa M and Chung S. Phenolic antioxidants isolated from mulberry leaves. Food Sci. Biotech, 2007, 16(5): 854-857.
Pan G and Cheng F. L. Isolation of a 1-aminocyclopropane-1-carboxylate oxidase gene from mulberry (Morus alba L.) and analysis of the function of this gene in plant development and stress response. Plant Physi, 2008, 165(11): 1204-1213.
Du J, He Z. D, Jiang R. W, Ye W. C, Xu H. X and But P. P. Antiviral flavonoids from the root bark of Morusalba L. Phytochem, 2003, 62(8): 1235-1238.
Harauma A, Murayama T, Ikeyama K, Sano H, Arai H and Takano R. Mulberry leaf powder prevents atherosclerosis in apolipoproteine deﬁcient mice. Biochem. Biophys. Res, 2007, 358(3): 751-756.
Jiang Y and Nie W. J. Chemical properties in fruits of mulberry species from the Xinjiang province of China. Food Chem, 2015, 174: 460-466.
Liu X, Xiao G, Chen W, Xu Y and Wu J. Quantification and purification of mulberry anthocyanins with Macroporous Resins Biom. Biotech, 2004, 2004(5): 326-331.
Katsube T, Imawaka N, Kamano Y, Yamazaki Y, Shiwaku K and Yamane Y. Antioxidant flavonol glycosides in mulberry (Morus alba L.) leaves isolated based on LDL antioxidant activity. Food Chem, 2006, 97(1): 25-31.
Zhang M, Wang F, Liu R, Tang X, Zhang Q and Zhang Z. Effects of superfine on physicochemical and antioxidant properties of Lyciumbarbarum polysaccharides. Food Sci., Tech, 2014, 58(2): 594-601.
Zhu Y, Dong Y, Qian X, Cui F, Guo, Zhou X, Wang Y, Zhang Y and Xiong Z. Effect of Superfine Grinding on Antidiabetic Activity of Bitter Melon Powder Mol. Sci, 2012, 13(11): 14203-14218.
Zhu F. M, Bin D and Jun L. Effect of ultrafine grinding on physicochemical and antioxidant properties of dietary fiber from wine grape pomace. Food Sci and tech, 2014, 20(1): 55-62.
Ming J, Chen L, Hong H and Li J. Effect of superfine grinding on the physico-chemical, morphological and thermogravimetric properties of Lentinusedodes mushroom powders. Sci. Food Agri, 2014, 95(12): 2431-2437.
Wu G, Zhang M, Wang Y, Mothibe K. J and Chen W. Production of silver carp bone powder using superfine grinding technology: Suitable production parameters and its properties. Food Engi, 2012, 109(4): 730-735.
Song L. L, Fan B. Y, Zong J and Zhang D. L. Probe into characteristics of Taraxacummongolicum ultramicro-power Chinese Mat. Med, 2002, 27(1): 12-15.
Muhammad A, Rasool K, Anna R, Ryszard, Amarowicz. Antioxidant Activity of Mulberry Fruit Extracts. Int. J. Mol. Sci, 2012, 13: 2472-2480.
Zou Y, Liao S, Shen W, Liu F, Tang C, Chen C. Y. O and Sun Y. Phenolic and antioxidant activity of Mulberry Leaves depend on Cultivar and Harvest Month in southern China. Mol. Sci, 2012, 13(12): 16544-16553.
Soni N, Mehta S, Satpathy G and Gupta R. K. Estimation of nutritional, phytochemical, antioxidant and antibacterial activity of dried fig (Ficuscarica). Pharm. phytochem., 2014, 3: 158-165.
Mudasir S, Pawankumar V, Rajinder R, Shahid P and Dar M. A. Quantitative analysis of total phenolic, flavonoids and tannin contents in acetone and n-hexane extracts of Ageratum conyzoides. Int. J. of Chemtech Res, 2012, 4(3): 974-4290.
Hussain K, Ismail Z, Sadikun A and Ibrahim P. Analysis of proteins, polysaccharides, glycosaponins contents of piper sarmentosum Roxb and anti-TB evaluation of bio-enhancing/interaction effects of leaf extracts with Isoniazid (INH). Nat. Prod. Radi, 2008, 7(5): 402-408.
Biju J, Sulaiman C. T and Satheesh G. Spectrophotometric of estimation of total alkaloids in selected justicia species. Pharm, 2014, 6(5): 647-648.
Kalaivani M, Jebanesan A, Maragathavalli S, Annaduirai B and Gangwar S. K. Studies on chlorophyll content, soluble protein, carbohydrates and moisture content of Morusalba Linn. Sci. Nat, 2013, 4: 131-137.
Dzomba P, and Musekiwa C. Anti-obesity and antioxidant activity of dietary flavonoids from Dioscoreasteriscustubers. Coast. Life Med, 2014, 2(6): 465-470.
Fu M, He Z, Zhao Y, Yang J. and Mao L. Antioxidant properties and involved compounds of daylily flowers in relation to maturity. Food chem, 2009, 114(4): 1192-1197.
Smirnoff N and Cumbes Q. J. Hydroxyl radical scavenging activity of compatible solutes. Phytochem, 1996, 4(2): 1057-1060.
Rajamanikandan S, Sindhu T, Durgapriya D, Sophia D, Rangavendran P and Gopalakrishnan V. K. Radical scavenging and antioxidant activity of ethanol extract of Mollugonudicaulis by in vitro assays. Indian J. of Pharm Res, 2011, 45(4): 310-316.
Hu J. H, Chen Y. Q. and Ni D. J. Effect of superfine grinding on quality and antioxidant property of fine green tea powders. Food Sci. Tech, 2012, 45(1): 8-12.
Jia Z, Tang M and Wu J. The determination of flavonoid content in mulberry and their scavenging effects on superoxide radicals. Food chem, 1999, 64(4): 555-559.
Marija M, Radojkovic, Zoran P, Zekovic, Senka S, Vidovic Kočar D and Pavle Z. Free radical scavenging activity and total phenolic and flavonoid contents of mulberry (Morus spp. L., Moraceae) extracts. Ind, 2012, 66(4): 547-552.
Zhong C, Zu Y, Zhao X, Li Y, Ge Y, Wu W and Zhang Y. Effect of superfine grinding on physical and antioxidant properties of pomegranate peel. Food Sci. Tech, 20165, 1(1): 212-221.
Chen Y, Ch B, Zhang, Sun Y. H, Zhang J. G, Sun H. J and Wei Zh. Physicochemical properties and adsorption of cholesterol by Okra (Abelmoschus esculentus) powder. J. Food Funct, 2015, 6(12): 3728.
Iqbal S, Younas U, Sirajuddin, Chan K. W, Sarfaraz R. A and Uddin K. Proximate composition and antioxidants potential of leaves from three varieties of mulberry (Morus sp.): A comparative study. Mol Sci, 2012, 13(6): 6651-6064.
Zhang L. L, Wang Y. M, Xu M, Wu D. M and Chen J. H. Antioxidant activity, phenol and flavonoid contents of fourteen Mulberry varieties leaves. Adv. Mat. Res, 2013, 781-783: 1454-1459.
Liang L, Wu X, Zhu M, Zhao W, Li F, Zou Y, and Yang L. Chemical composition, nutritional value, and antioxidant activities of eight mulberry cultivars from China. Pharm. Mag, 2012, 8(31): 215-24.
Li F, Qiu Y, Qian W, Lou L, Zeng S and Jiang H. Determination and Investigation of total alkaloids and 1-deoxynojirimyc in Folium Mori. Chinese J. of Pharm, 2008, 43: 3-4.
Dhiraj K and Venkatesh K. R. Efficacy of bio-foliar-spray on growth and biochemical parameters of different mulberry varieties. Biol Sci., 2014, 14(1): 64-69.
Manisankar G, Aniruddha M and Ujjal K. M. Studies on the effect of Mulberry leaves of Lune variety on Silkoworm (Bombyx Morri) rearing in polluted environment of gangetic plains of west Benga. Res. Chem. Sci., 2011, 1: 80-89.
Bruno M. M, Constant A. P, Jacques R. N, Cabral P. N. B, Bravi M and Jeanne Y.N. In vitro antioxidant properties, free radicals scavenging activities of extracts and polyphenol composition of a non-timber forest product used as spice: Monodora myristica. Moukette et al. Biological Research, 2015, 48-15.