Biochemistry and Molecular Biology
Volume 4, Issue 5, September 2019, Pages: 67-73
Received: Oct. 8, 2019;
Accepted: Nov. 8, 2019;
Published: Nov. 15, 2019
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Yao Ruoyu, Graduate School of Xuzhou Medical University, Xuzhou, China
Zhan Hao, Xuzhou Tumor Hospital, Xuzhou, China
Zhang Xuguang, Graduate School of Xuzhou Medical University, Xuzhou, China; Xuzhou Tumor Hospital, Xuzhou, China
[Objective] To observe the retransmission of radiation side effects between cells. [Materials and Methods] The mouse ovarian cancer cell line NUTU19 was irradiated with 6MV-X-rays, and the culture medium was prepared for the first-generation conditioned medium. The first-generation effector cells were used to detect the NUTU19 cell line and the intestinal mucosal epithelial IEC-6 cell line. The secretion of effector cells was a second-generation conditioned medium, and the second-generation effector cells NUTU19, IEC-6, and mouse lymphocytes were treated to measure cell viability and apoptosis. [Results] After treated with NUTU19 second-generation medium for 48 hours, the apoptosis rate of IEC-6 and NUTU19 cells was promoted (p>0.05), and the apoptosis rate of lymphocytes was decreased (p<0.05). After treatment with NUTU19 second-generation medium for 72 h, the apoptosis of NUTU19 and IEC-6 was promoted (p<0.05), and there was no effect on the apoptosis rate of lymphocytes (p>0.05). After treatment with IEC-6 second-generation medium for 48h, the apoptosis of NUTU19 and IEC-6 was promoted (p<0.05), and the apoptosis of lymphocytes was decreased (p<0.05). After treated with IEC-6 second-generation medium for 72h, there was no effect on IEC-6 and lymphocyte apoptosis (p>0.05). It promoted the apoptosis of NUTU19 (p<0.05). [Conclusion]: Under certain conditions, tumor cells, intestinal epithelial cells and lymphocytes can retransmit the damage of the side effect of radiation.
Experimental Observation on the Retransmission of Radiation Side Effects, Biochemistry and Molecular Biology.
Vol. 4, No. 5,
2019, pp. 67-73.
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