Antagonism of Dexamethasone to Radiation Bystander Effect and Its Re-transmission
Biochemistry and Molecular Biology
Volume 4, Issue 5, September 2019, Pages: 74-79
Received: Oct. 8, 2019;
Accepted: Nov. 8, 2019;
Published: Nov. 15, 2019
Views 475 Downloads 94
Zhan Hao, School of Clinical, Graduate School of Xuzhou Medical University, Xuzhou, China
Yao Ruoyu, School of Clinical, Graduate School of Xuzhou Medical University, Xuzhou, China
Zhang Xuguang, School of Clinical, Graduate School of Xuzhou Medical University, Xuzhou, China; Department of Radiotherapy, Xuzhou Cancer Hospital, Xuzhou, China
Background: In clinical radiotherapy, in addition to the symptoms of radiation damage in the irradiated area, normal tissues can produce damage other than direct irradiation due to bystander effects. We often use dexamethasone to relieve symptoms, thereby protecting the surrounding normal tissues, and may also protect the tissues outside the target area. Objectives: To observe the antagonism of dexamethasone on radiation bystander effect and its Re-transmission. Materials and methods: After 6MV X-ray irradiation of rabbit, the plasma, the first generation conditioned medium, was prepared to treat rabbit lymphocyte, cultured for 24 hours, 24 hours later, lymphocytes were collected, and the second generation conditioned medium was prepared with the lymphocyte secretion to treat new rabbit lymphocyte, and the apoptotic rate of effecter lymphocyte was detected. Dexamethasone was used as a parallel treatment control in all experimental groups. Results: The first and second generations of conditioned medium can stimulate lymphocyte apoptosis. Dexamethasone could reduce the apoptotic level of rabbit lymphocytes treated with the first and second generation bystander conditioned medium from 21.087% to 14.957%, 28.695% to 20.205%, respectively. Conclusion: Under certain conditions, dexamethasone can antagonize the injury of lymphocyte by radiation bystander effect, and can also antagonize the re-transmission of radiation bystander effect.
Antagonism of Dexamethasone to Radiation Bystander Effect and Its Re-transmission, Biochemistry and Molecular Biology.
Vol. 4, No. 5,
2019, pp. 74-79.
Wersall PJ, Blomgren H, Pisa P, et a1. Regression of non—irradiated metastases after extracranial stereotactic radiotherapy in metastatic renal cell carcinoma [J]. ActaOncol, 2006, 45 (4): 493-497. DOI: 10.1080/0284l86O6006046l1.
Konoeda K, Therapeutic efficacy of pre—operative radiotherapy on breast carcinoma: in special reference to its abscopal effecton metastatic lymph-nodes lJI. Nihon Gan Chiryo Gakkai shi, 1990, 25 (61): 1204-1214.
Camphausen K, Moses MA, Menard C, et al. Radiation abscopal antitumor effect is mediated throughp53 [J]. Cancer Res, 2003, 63 (8): 1990-1993.
Yang S, Jing X, Shao W, et al. Radiation-Induced Bystander Effects in A549 Cells Exposed to 6 MV X-rays.[J]. Cell Biochemistry & Biophysics, 2015, 72 (3): 1-6.
Siva S, Lobachevsky P, Macmanus MP, et al. Radiotherapy for nonsmall cell lung cancer induces DNA damage response in both irradiated and out-of-field normal tissues [J]. Clin Cancer Res, 2016, 22 (19): 4817-4826. DOI: 10.1158/1078-O432.CCR-16-0138.
Khan M A, Hill R P, Van Dyk J. Partial volume rat lung irradiation: an evaluation of early DNA damage [J]. Int J Radiat Oncol Biol Phys, 1998, 40 (2): 467-76.
Calveley V L, Jelveh S, Langan A, etal. Genistein can mitigate the effect of radiation on rat lung tissue [J]. Radiat Res, 2010, 173 (5): 602-11.
Koturbash I, Rugo R E, Hendricks C A, etal. Irradiation induces DNA damage and modulates epigenetic effectors in distant bystander tissue in vivo [J]. Oncogene, 2006, 25 (31): 4267-75.
Koturbash I, Zemp FJ, Kutanzi K, Luzhna L, Loree J, Kolb B, Kovalchuk O. Sex-specific micro RNAome deregulation in the shielded bystander spleen of cranially exposed mice. [Cell Cycle. 2008 Jun 1; 7 (11): 1658-67. Epub 2008 Mar 23.
Tamminga J, Koturbash I, Baker M, et al. Paternal cranial irradiation induces distant bystander DNA damage in the germline and leads to epigenetic alterations in the offspring [J]. Cell Cycle, 2008, 7 (9): 1238-45.
Aasland D, Reich TR, Tomicic MT, Switzeny OJ, Kaina B, Christmann M. Repair gene O6 -methylguanine-DNA methyltransferase is controlled by SP1 and up-regulated by glucocorticoids, but not by temozolomide and radiation. J Neurochem. 2018 Jan; 144 (2): 139-151. doi: 10.1111/jnc.14262. Epub 2018 Jan 1.
Luedi MM, Singh SK, Mosley JC, Hatami M, Gumin J, Sulman EP, Lang FF, Stueber F, Zinn PO, Colen RR. A Dexamethasone-regulated Gene Signature Is Prognostic for Poor Survival in Glioblastoma Patients. J Neurosurg Anesthesiol. 2017 Jan; 29 (1): 46-58.
Ortega-Martínez S. Dexamethasone acts as a radiosensitizer in three astrocytoma cell lines via oxidative stress. Redox Biol. 2015 Aug; 5: 388-97. doi: 10.1016/j.redox.2015.06.006. Epub 2015 Jun 23.
Rane JK, Erb HH, Nappo G, Mann VM, Simms MS, Collins AT, Visakorpi T, Maitland NJ. Inhibition of the glucocorticoid receptor results in an enhanced miR-99a/100-mediated radiationresponse in stem-like cells from human prostate cancers. Oncotarget. 2016 Aug 9; 7 (32): 51965-51980. doi: 10.18632/oncotarget.10207.
Furfari A, Wan BA, Ding K Wong A, Zhu L, Bezjak A, Wong R, Wilson CF, DeAngelis C, Azad A, Chow E, Charames GS. Genetic biomarkers associated with pain flare and dexamethasone response following palliative radiotherapy in patients with painful bone metastases. Ann Palliat Med. 2017 Dec; 6 (Suppl 2): S240-S247. doi: 10.21037/apm.2017.09.04. Epub 2017 Sep 20.
Weidenfeld J, Siegal T, Ovadia H. Delayed effects of brain irradiation--part 1: adrenocortical axis dysfunction and hippocampal damage in an adult rat model. Neuroimmunomodulation. 2013; 20 (1): 57-64. doi: 10.1159/000342522. Epub 2012 Nov 23.
Surace L, Lysenko V, Fontana AO, Cecconi V, Janssen H, Bicvic A, Okoniewski M4, Pruschy M, Dummer R, Neefjes J, Knuth A, Gupta A, van den Broek M. Complement is a central mediator of radiotherapy-induced tumor-specific immunity and clinical response. Immunity. 2015 Apr 21; 42 (4): 767-77. doi: 10.1016/j.immuni.2015.03.009. Epub 2015 Apr 14.
Alt C, Runnels JM, Mortensen LJ, Zaher W, Lin CP. In vivo imaging of microglia turnover in the mouse retina after ionizing radiation and dexamethasone treatment. Invest Ophthalmol Vis Sci. 2014 Jul 31; 55 (8): 5314-9. doi: 10.1167/iovs.14-14254.
Fernanda G. Herrera, MD; Jean Bourhis, MD, PhD; George Coukos, MD, PhD. Radiotherapy Combination Opportunities Leveraging Immunity for the Next Oncology Practice [J]. CA CANCER J CLIN 2017; 67: 65–85.
Wayne J. Aston, Danika E. Hope, Alistair M. Cook, Louis Boon, Ian Dick, Anna K. Nowak, Richard A. Lake, and W. Joost Lesterhuis. Dexamethasone differentially depletes tumour and peripheral blood lymphocytes and can impact the efficacy of chemotherapy/checkpoint blockade combination treatment. ONCOIMMUNOLOGY 2019, VOL. 8, NO. 11. 2019 July 4.
Robe PA, Nguyen-Khac M, Jolois O, Rogister B, Merville MP, Bours V. Dexamethasone inhibits the HSV-tk/ganciclovir bystander effect in malignant glioma cells. BMC Cancer. 2005 Apr 2; 5: 32.