Exploration on Mixed Cultivation of Lactobacillus, Yeast and Bacillus subtilis
Chemical and Biomolecular Engineering
Volume 1, Issue 2, December 2016, Pages: 45-48
Received: Dec. 3, 2016;
Accepted: Dec. 16, 2016;
Published: Jan. 17, 2017
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Sai Zhao, Department of Biopharmaceutical, College of Basic Sciences, Tianjin Agricultural University, Tianjin, China
Yan-Ling Wang, Department of Biopharmaceutical, College of Basic Sciences, Tianjin Agricultural University, Tianjin, China
Tao Li, Department of Biopharmaceutical, College of Basic Sciences, Tianjin Agricultural University, Tianjin, China
Shu-Lin Zhang, Key Laboratory of Aquatic Ecology and Aquaculture of Tianjin, Tianjin, China
Fang-Di Cong, Department of Biopharmaceutical, College of Basic Sciences, Tianjin Agricultural University, Tianjin, China; Key Laboratory of Aquatic Ecology and Aquaculture of Tianjin, Tianjin, China
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To study on mixed fermentation of probiotics, bacterial cultivation was carried out firstly. It was showed that the optimal pH values for growth of Lactobacillus, Yeast and Bacillus subtilis were 6.5, 6.0 and 7.0, respectively. And the suitable temperature for all of them was about 31°C. Then under selective conditions pH 6.0, 31°C and 160 r/min, three probiotics were cultivated by taking Lactobacillus as main bacteria and the others as auxiliaries. It was found that the desired bacterial number ratio of Lactobacillus: Yeast: Bacillus for mixed cultivation was 1:2:2 and the OD value of mixed bacterial solution achieved at 2.27 after three days.
Mixed Cultivation, Lactobacillus, Yeast, Bacillus subtilis
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Exploration on Mixed Cultivation of Lactobacillus, Yeast and Bacillus subtilis, Chemical and Biomolecular Engineering.
Vol. 1, No. 2,
2016, pp. 45-48.
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