Bioinsecticides Induce Change in Biochemical and Immunological Parameters of Spodoptera Littoralis Larvae
Chemical and Biomolecular Engineering
Volume 2, Issue 2, June 2017, Pages: 106-112
Received: Jan. 15, 2017; Accepted: Feb. 6, 2017; Published: Mar. 4, 2017
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Author
Badriah M. K. Asiri, Department of Biology, Faculty of Science for Girls, King Abdulaziz University, Jeddah, Saudi Arabia
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Abstract
The cotton leaf worm, Spodoptera littoralis, is a major pest in Egypt causes severe quantitative and qualitative losses of cotton and other economic crops. This study examines the effect of Caltropis proceraa and Atriplex halimus extracts on the 4nd instar larvae. This study was carried out to identify the effect plant extracts on biochemical parameters and differential and total haemocyte counts of S. littoralis after treated with LC25 of Caltropis proceraa and Atriplex halimus extracts for 48 hours (treated with each extract in separate). Marked biochemical changes, however, being recognized in pest as marked decrease in total lipids, total protein and glucose contents. The activities of both aspartate amino transferase (ASAT) and alanine amino transferase (ALAT) are also being highly affected. Four types of haemocytes marked by; prohaemocytes (PRs), plasmatocytes (PLs), granulocytes (GRs) and oenocytoids (OEs). The percentage of PRs decreased in insects fed on leaves treated with Caltropis proceraa and Atriplex halimus plant. The percentage of PLs increased, while the percentage of GRs decreased in all tested insects treated. The percentages of oenocytoids (OEs) increased in insect fed treated compared with control. Results indicated that total Haemocyte Counts (THCs) of insects fed on leaves treated significantly decreased in all insects treated.
Keywords
Spodoptera Littoralis Larvae, Caltropis Proceraa, Atriplex Halimus, Biochemical, Immunological Parameters
To cite this article
Badriah M. K. Asiri, Bioinsecticides Induce Change in Biochemical and Immunological Parameters of Spodoptera Littoralis Larvae, Chemical and Biomolecular Engineering. Vol. 2, No. 2, 2017, pp. 106-112. doi: 10.11648/j.cbe.20170202.15
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Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
References
[1]
Amin, A and Salam, I. (2003). Factors stimulating the outbreaks of the cotton leafworm in Assuit Governorate. Bel Twide Cotton Conferences, Nashville, TN-January, 6 (10): 1420-1422.
[2]
Abo-Elghar, G. E., Zeinab, A., Elbermawy, Y. A. G., Abd Elhady, H. K. (2005). Monitoring and characterization on insecticide resistance in the cotton leafworm, S. littoralis (Boisd.) (Lepidoptera, Noctuidae). Journal of Asia-Pacific Entomology, 8 (4): 397-410.
[3]
Atwa, W. A., Adel, M. M., Salem, N. Y., Abdou, W. L., and Ibrahim, S. S., (2010). Some Physiological and Histopathological Studies of NeemAzal T/S and Two Wild Egyptian plant extracts on the black cutworm Agrotis ipsilon (Hufn.) (Lepid., Noctuidae). Bulletin of NRC. Egypt, 35 (1): 15-32.
[4]
Shonouda ML, Osman SL (2000). New botanical derivatives, used in medicinal reparations, showing bioactive action on insect pests. 1-Toxicological effect on the development of Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae). J. Egypt. Ger. Soc. Zool., 31: 227-234.
[5]
Khawas MAM and Abd El-Gawad HAS (2002). The efficiency of two plant extracts (Fenugreek and Lupine) and commercial biofungicide (Biofly) on the cotton leafworm, Spodoptera Littoralis (Boisd) (Lepidoptera: Noctuidae) larvae as a new approach of control. J. Egypt. Ger. Soc. Zool., 37: 39-57.
[6]
Rawi SM, El-Gindy H, Haggag AM, Abou El Hassan, A. Abdel Kader, A. (1995). Few possible molluscicides from calendula Micrantha officinalis and Ammi majus plants. I. Physiological effect on B. alexandrina and B. truncatus. J. Egypt. Ger. Soc. Zool., 16 69-75.
[7]
Rawi SM, El-Gindy H, Abd El-Kader A (1996). New possible Molluscicides from Calendula micrantha officinalis and Ammi majus. II: Molluscicidal, physiological and egg laying capacity against B. alexandrina and B. truncates. J. Ecotoxicol. Environ. Safety. 35 261-267.
[8]
Brumo M, Piozzi AM, Puech F, Rosselli, S., Simmonds, M. S. J. (2003). Neoclerodane diterpenoids from Teucrium poloum sub sp. Poloum and their antifeedant activity. Biochemical Systematics and Ecology, 1051-1056.
[9]
Sadek MM (2003) Antifeedant and toxic activity of Adhatoda vesica leaf extract against Spodoptera littoralis (Lep. Noctuidae). J. Applied Entomology, 127: 396-404.
[10]
Nakatani M, Abd El-Galeil SAM, Kurawaki J, Okamura H, Iwagawa T, Doe M (2001) Antifeedant ring B and D opened limonoids from Khaya senegalensis. J. of Natural products. 64: 1263-1265.
[11]
Schmidt GH, Assembe-Tsoungui S (2002) Effect of oral application of Melia fruit extract on growth, development and gregarization of the desert locust Schistocerca gregaria (Forsk) (Caelifera, Acridiae). Zeitschrift fur Pflanzenkrankheiten und Pflanzenachutz. 109: 38-56.
[12]
Farag NA (2002) Effect of Haphyllum tuberculatum extracts on an insect on an insect pest Spodoptera littoralis and the predator, Coccinella und ecimpunctata. Ann. Of Agric. Sci. Cairo. 47: 1097-1105.
[13]
Ghoneim KS (1985) Physiological studies on endocrine and reproductive system of the cotton leafworm, Spodoptera littoralis (Boisd) (Lepidoptera: Noctuidae). ph. D. Thesis, Al-Azhar Univ., Cairo.
[14]
Freedman B, Nowak LJ, Ewalek WF, Berry EC, Guthrie 118 Int. Res. J. Plant Sci. WDA (1979). bioassay for plant derived pest control agents using European Corn borer. J. Econ. Ent., 72 541-545.
[15]
Abbott WS (1925) A method of computing the effectiveness of an insecticide. J. Econ. Ent. 18: 265-267.
[16]
Dempster C (1957) The population dynamic of Moroccan locust Dociostarus marcocanus in Cyprus, Antilocust Bull.
[17]
Funny DJ (1971). Probit Analysis, 3rd Ed., Camridge Univ. Press, London 3.
[18]
Reitman S, Frankel S, Amer. J (1957) Clin. Path., 28: 56. Roger C, Vincent C, Coderra D (1995) Mortality and predation efficiency of Coleomegilla maculate lengi Timb. (Col., Coccinellidge) following application of neem extracts (Azadirachta indica A. Juss., Meliaceae). J. App. Ent., 119: 439-443.
[19]
Trinder P (1969) Ann. Clin. Biochem. 6: 24. Younes MWF, Abd-Dahab FF, Assar AA (1999). Histopathology studied on the effect of some botanical extracts on the cotton leafworm, Spodoptwea littoralis (Boisd) (Lepidoptera: Noctuidae) II –effect on rhe integument, the midgut and fat bogy. 2th Sci. Conf. on the role of science in the development of Egyptain Society and Enviroment, Zagazig Unif/. Fac. Of Sci., Benha, 113-129.
[20]
Zollner N, Kirsch K, Ges Z (1962) Exp. Med. 135 545. Zidan ZH, El-Hammady A, Radwan SM, Abdel-All SM, Aly MM (2000). Enzyme and biochemical responses of cotton leafworm larvae to the tested Eucalyptus plant extract in laboratory. Annals of Agricultural Science Cairo. 45: 793-810.
[21]
Hoffmann, D. (1980). Induction of antibacterial activity in the blood of the migratory locust, Locusta migratoria L. Journal of Insect Physiology, 26: 539-549.
[22]
Jones, J. C. (1962): Current concepts concerning insect haemocytes. American Zoologist, 2: 209-246.
[23]
Dixon WJ, Massay JF (1957) Introduction to Statistical Analysis. 2nd Ed., MC Graw-Hill book Co., Inc, Newyork.
[24]
Badr NA, Sondos A M, Abd El-Haleem SM (2000). Effect of seed oil extracts on the different developmental stages of the Egyptian cotton leafworm, Spodoptera littoralis (Boisd).10 39-50. data. 1- Tissues affected in larvae. Proc. 2nd Int. Neem Conf.., Rauisehholzhausen, 227-236.
[25]
Schmutterer H (1988) Potential of azadirachtin containing pesticides for integrated pest control in developing and industrialized countries, J. Ins. Phys. 34 713-719.
[26]
Kogam A (1986) Natural chemicals in plant resistance to insect. Towa state J. of research, 60: 501-527.
[27]
Klock JA, Chan B (1986) Chemistry and Technology of Agrochemical formulations. Kluwer Academic Published Dordrecht/Boston/London.
[28]
Fayez A B, Karem H, Mahmoud and Somaya M I.(2016) Alterations in the fatty acid profile, antioxidant enzymes and protein pattern of Biomphalaria alexandrina snails exposed to the pesticides.
[29]
diazinon and profenfos. Toxicology and Industrial Health 2016, Vol. 32 (4) 666–676.
[30]
Nabih L, El-Dardiri Z, El-Ansary A, Ahmed SA (1990) Kinetic properties of two transaminases and lactate dehydrogenase in fresh water snails specific intermediate host for human schistosomiasis, Cell. Mol. Biol. pp. 36375.
[31]
Ahmed NA, Rawi SM, Radwan NM, Attla MH (1993). Comparative effects of hostathione, endrin, Lannate and sumicidin on the transaminases activity of liver, brain and plasms of frog Rana ridibunda. Bull. Fac. Sci. Cairo Univ. 6093-110.
[32]
Hanan F A, Maha Z R, Dina M, MM, Desoky, NA I and Eman A Y.(2016). Therapeutic and protective effects of Caesalpinia gilliesii and Cajanus cajan proteins against acetaminophen overdose-induced renal damage. Toxicology and Industrial Health 2016, Vol. 32 (4) 753–768.
[33]
Abo El-Ela RG, Helmy NM, Monairy QM, Salah H (1997) Effect ofcertain plant extracts on some biochemical aspects of the housy fly larvae Musca domestica (Dipetra Muscidae). Bull. EntSoc. Egypt, Econ. Ser., 2217-25.
[34]
Chitra KC, Reddy TSV (2000) Effects of Annona squamosa L. seed extract on protein metabolism of Spodoptera liture. Fab. Insect Environmental. 6: 39-40.
[35]
Hashem AM, El- Ansary A, Faddah LM (1993) Effect of some organophorus insecticides on amylases and cellulases in Biophalaria alexandrina snails. Egypt. J. Bilh, 219: 35.
[36]
Mostafa SA (1993) Biochemical effect of some chemical compound on Spodoptera littoralis (Boisd). pH. D. Thesis, Fac. Agric., Al-Azhar Univ., Egypt.
[37]
Anitha B, Arivalagan M, Sundari MSN, Durairaj G (1999) Effect of alkaloid isolated from Abrus precatorius Linn. Seeds on mealy bug, Maconellicoccus hirsutus Green. Indian J. of Exp., Bio., 37: 415-417.
[38]
Zhu, Q.; He, Y.; Yao, J.; Liu, Y.; Tao, L. and Huang, Q. (2012): Effects of sublethal concentrations of the chitin synthesis inhibitor, hexaflumuron, on the development and hemolymph physiology of the cutworm, Spodoptera litura. Journal of Insect Science, 12 (27): 1-13.
[39]
Szymaś B. and Jędruszuk, A. (2003): The influence of different diets on haemocytes of adult worker honey bees, Apis mellifera. Apidologie, 34: 97–102.
[40]
Anandakumar, M. D. and Michael, A. S. (2011): Haematology and haemochemistry of silkworm, Bombyx Mori L. infected with Bacillus thuringiensis. International Journal of Environmental Sciences, 2 (2): 451-457.
[41]
Hillyer, J. F.; Schmidt, S. L.; Fuchs, J. F.; Boyle, J. P. and Christensen, B. M. (2005): Age-associated mortality in immune challenged mosquitoes (Aedes aegypti) correlates with a decrease in haemocyte numbers. Cellular Microbiology, 7: 39–51.
[42]
Alaux, C.; Ducloz, F.; Crauser, D. and Le Conte, Y. (2010): Diet effects on honeybee immunocompetence. Biology letters, 6 (4): 562-565.
[43]
Silva, J. E. B.; Boleli, I. C. and Simões, Z. L. P. (2002): Hemocyte types, total and differential counts in unparasitized and parasitized Anastrepha oblique (Diptera, Tephridae) larvae. Brazilian Journal of Biology, 62: 689-699.
[44]
Andrade, F. G.; Negreiro, M. C. C.; Levy, S. M.; Fonseca, I. C. B.; Moscardi, F. and Falleiros, A. M. F. (2010): Hemocyte quantitative changes in Anticarsia gemmatalis (Lepidoptera: Noctuidae) larvae infected by AgMNPV. Brazilian Archives of Biology and Technology, 53 (2): 279-284.
[45]
Banville, N.; Browne, N. and Kavanagh, K. (2012): Effect of nutrient deprivation on the susceptibility of Galleria mellonella larvae to infection. Virulence, 3 (6): 497–503.
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