Identification of Animal Muscle Proteins by 2D-Electrophoresis and Time-of-Flight Mass Spectrometry
Chemical and Biomolecular Engineering
Volume 2, Issue 2, June 2017, Pages: 96-105
Received: Jan. 14, 2017;
Accepted: Jan. 25, 2017;
Published: Mar. 4, 2017
Views 1897 Downloads 100
Natalia Leonid Vostrikova, Department of Analytical Research, the V.M. Gorbatov All-Russian Meat Research Institute, Moscow, Russia
Andrew Vladimir Kulikovskii, Department of Analytical Research, the V.M. Gorbatov All-Russian Meat Research Institute, Moscow, Russia
Irina Mihail Chernuha, Department of Analytical Research, the V.M. Gorbatov All-Russian Meat Research Institute, Moscow, Russia
Andrew Nikolay Ivankin, Department of Chemistry, Bauman Moscow State Technical University, Moscow, Russia
Follow on us
A method for determining the quantitative content of meat proteins by two-dimensional electrophoresis (2D). The method is designed to quantify muscle protein in raw meat samples through proteomic technology complex comprising a two-dimensional polyacrylamide gel electrophoresis and densitometry obtained electrophoregrams computer.
Proteomics, Two-Dimensional Electrophoresis, Biomarkers, Mass Spectrometry
To cite this article
Natalia Leonid Vostrikova,
Andrew Vladimir Kulikovskii,
Irina Mihail Chernuha,
Andrew Nikolay Ivankin,
Identification of Animal Muscle Proteins by 2D-Electrophoresis and Time-of-Flight Mass Spectrometry, Chemical and Biomolecular Engineering.
Vol. 2, No. 2,
2017, pp. 96-105.
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/
) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Anderson N. L., Anderson N. G. 1998. Proteome and proteomics: new technologies, new concepts, and new words. Electrophoresis, 19: 1853-1861.
Blum H., Beir H., Cross H. 1987. Improved silver staining of plant proteins, RNA and DNA inpolyacrylamide gels. Electrophoresis, 8: 126-129.
Fairbanks G., Steck T. L., Wallach D. F. 1971. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry, 10: 2607-2617.
Hoffmann K. 1997. Identification and determination of meat and foreign proteins by means of dodecylsulfat poliacrylamid gel electrophoresis. Ann. Nutr. Alim., 31: 207-215.
O'Farrell P. H. 1975. High Resolution two-dimensional analysis of proteins. J. Biol. Chem., 250: 4007-4021.
Kitchen C. M. R. 2009. Nonparametric vs parametric tests oflocation in biomedical research. American Journal of Ophthalmology, 147: 571-572.
Govorun V. M., Moszkowski S. A., Tikhonova O. V. 2003. Comparative proteomic characterization of clinical isolates of cards. Biochemistry, 68: 52-58.
Kovaleva M. A., Ivanov A. V., Kovalev L. I., Shishkin S. S., Hriapova E. E., Lisitsin A. B., Chernuha I. M., Vostrikova N. L. 2012. Proteomic technology in research of protein composition of cooked sausages. All about meat, 2: 35-42.
Kovaleva M. A., Kovalev L. I., Shishkin S. S., Ivanov A. V., Vostrikova N. L., Chernuha I. M. 2013. Proteomic study of raw meat, cooked sausages and meat products of functional. Modern problems of science and education, 5: pp. 8-12.
Chernuha I. M., Vostrikovа N. L. 2011. Coll. Materials of the 14th Intern. Scien. Conf. "Promising areas of research in the field of processing of meat raw materials and the creation of competitive foods" V. M. Gorbatov memory. Moscow, VNIIMP: 239-241.
Lisitsyn A. B., Chernukha I. M., Ivankin A. N. 2013. Comparative study of fatty acid composition of meat material from various animal species. Scientific Journal of Animal Science, 2: 124–131.
Neklyudov A. D., Ivankin A. N., Berdutina A. V. 2000. Properties and uses of protein hydrolysates. Applied Biochemistry and Microbiology, 36: 452-459.