Antisickling, Antioxidant and Antibacterial Activities of Afromomum alboviolaceum (Ridley) K. Schum, Annona senegalensis Pers. and Mondia whitei (Hook. f.) Skeels
American Journal of Laboratory Medicine
Volume 2, Issue 4, July 2017, Pages: 52-59
Received: Aug. 24, 2017; Accepted: Sep. 20, 2017; Published: Oct. 17, 2017
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Authors
Gédéon Bongo, Department of Biology, University of Kinshasa, Kinshasa, Democratic Republic of the Congo
Clément Inkoto, Department of Biology, University of Kinshasa, Kinshasa, Democratic Republic of the Congo
Colette Masengo, Department of Environmental Sciences, University of Gbadolite, Nord-Ubangi, Democratic Republic of the Congo
Claudine Tshiama, Enseignement et Administration en Soins Infirmiers, Section Sciences Infirmières, Institut Supérieur des Techniques Médicales, Kinshasa, Democratic Republic of the Congo
Emmanuel Lengbiye, Department of Biology, University of Kinshasa, Kinshasa, Democratic Republic of the Congo
Ruphin Djolu, Department of Environmental Sciences, University of Gbadolite, Nord-Ubangi, Democratic Republic of the Congo
Mutwale Kapepula, Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of the Congo
Kabamba Ngombe, Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of the Congo
Théophile Mbemba, Department of Biology, University of Kinshasa, Kinshasa, Democratic Republic of the Congo
Dorothée Tshilanda, Department of Chemistry, University of Kinshasa, Kinshasa, Democratic Republic of the Congo
Pius Mpiana, Department of Chemistry, University of Kinshasa, Kinshasa, Democratic Republic of the Congo
Koto-te-Nyiwa Ngbolua, Department of Biology, University of Kinshasa, Kinshasa, Democratic Republic of the Congo; Department of Environmental Sciences, University of Gbadolite, Nord-Ubangi, Democratic Republic of the Congo; Ubangi Bio-Explore Project, Biodiversity Exploration of Ubangi River Bassin and Carbon Assessment, Nord-Ubangi, Democratic Republic of the Congo
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Abstract
In accordance with statistics from the World Health Organization, nearly 80% of populations depend on traditional medicine for primary health care. In order to scientifically validate the phyto-therapeutic wealth of the Democratic Republic of the Congo, three plants were chosen namely: Afromomum alboviolaceum, Annona senegalensis and Mondia whitei. The objective of our study was to assess the antisickling, antioxidant and antibacterial activities of the ethanolic extracts and the dichloromethane fractions of these three plants. Sickle cell blood was supplied from Centre de Médecine Mixte and Anémie SS of Yolo Sud, Kinshasa. Three bacterial strains were used including Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 8739 and Pseudomonas aeroginosa ATCC 9027. The ethanolic extracts of A. alboviolaceum and A. senegalensis and dichloromethane fractions of A. alboviolaceum and M. whitei showed IC50 values < 100 μg/mL in the ABTS test. Only the dichloromethane fraction of A. senengalensis showed an IC50 value of less than 100 μg/mL for the DPPH test. The inhibitory concentration 50 (IC50) values obtained from the ABTS test are lower than those of the DPPH test. All tested extracts possess a high antisickling activity and only soluble dichloromethane extracts are active vis-à-vis Staphylococcus aureus (MIC = 31.5 μg/mL). These results constitute a scientific evidence validating the use of these three medicinal plants for the management of sickle-cell anemia in the Democratic Republic of the Congo.
Keywords
Antisickling Activity, Antioxidant Activity, Antibacterial Activity, Inhibitory Concentration, ABTS, DPPH
To cite this article
Gédéon Bongo, Clément Inkoto, Colette Masengo, Claudine Tshiama, Emmanuel Lengbiye, Ruphin Djolu, Mutwale Kapepula, Kabamba Ngombe, Théophile Mbemba, Dorothée Tshilanda, Pius Mpiana, Koto-te-Nyiwa Ngbolua, Antisickling, Antioxidant and Antibacterial Activities of Afromomum alboviolaceum (Ridley) K. Schum, Annona senegalensis Pers. and Mondia whitei (Hook. f.) Skeels, American Journal of Laboratory Medicine. Vol. 2, No. 4, 2017, pp. 52-59. doi: 10.11648/j.ajlm.20170204.13
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Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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