Monitoring the Activity of Feed Enzymes in Vitro and Their Activity in the System that Modulates the GIT
Ecology and Evolutionary Biology
Volume 4, Issue 3, September 2019, Pages: 33-38
Received: Jun. 26, 2019; Accepted: Sep. 12, 2019; Published: Oct. 23, 2019
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Valeriy Sergeevich Kryukov, LLC Vetpharmstandart, Moscow, Russian Federation
Ilona Vyacheslavovna Glebova, Kursk State Agricultural Academy, Kursk, Russian Federation
Sergej Vladimirovich Zinoviev, FSC "VNITIP" RAS, Sergiev Posad, Moscow, Russian Federation
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24 feed enzyme endo-1,4-glucanase (glucanase) and endo-1,4-xylanase (xylanase) have been described. Their activity was studied under in vitro conditions simulating the gastrointestinal tract of the poultry. A decrease in the temperature of the medium from 50°C to 38°C reduced the activity of all enzymes. Fekord 2004-S, Agrocell Plus, Agrocell, Rovabio max AP, Xibeten-Gel, Axtra XAP 101 TPT, Endofeed DC at pH 3, 38°C retained the highest initial xylanase activity: 81, 80, 70, 70, 67, 67, 65% respectively. The minimum stability at pH 3 was established for Sunzyme, Hostazym С-100, Ronozyme VP, Natugrain TS and Ronozyme WX: 46, 44, 42, 40, and 38%, respectively. At pH 7, 38°C it was better activity than the average for all preparations showed С Axtra XAP 101 TPT, Fekord-2004-C, Econase XT-25, Agrocell: 52, 47, 47, 45%; minimal activity was shown by Rovabio Exel AR, Cellulase, Cellolux F, Sunzaim: 4, 5, 7, and 8%, respectively. An outstanding high activity of cellulаse at pH 3, 38°C was found in Fekord 2004-C - 90%, above the average value it was in Econase XT 25, Acra XB 201 TPT, Agroxil Plus and Vilzim: 63, 62, 60 and 58%. The minimum activity at pH 3, 38°С was found in Rovabio Exel, Rovabio Max AR, Cellolux F and Xybeten-Cel: 20, 23, 23 and 25%. At pH 7, 38°С, the maximum activity of most enzymes remained within 30%, excluding Hostazym S-100 - 52%. The minimum glucanase activity at pH 7 was shown by Rovabio Max AP - 4%, Xybeten-Cel - 5%, Endofeed DC - 6%, and Rovabio Exel AP -6%. The average activity of cellulase for all studied enzymes was 19.1% of that determined at pH 5, and 50C. Under all experimental conditions, the activity of xylanases of the studied enzymes was mainly better than glucanases. This indicates greater cellulases stability. It has been substantiated that the activity indicated by the manufacturer serves only for labeling the product and does not reflect its action in the body. Used matrix values of enzymes are intended for commercial purposes and weakly reflect the real effect of enzyme.
Feed Enzymes, Activity Enzymes, Choice Feed Enzymes, Matrix Values Enzymes
To cite this article
Valeriy Sergeevich Kryukov, Ilona Vyacheslavovna Glebova, Sergej Vladimirovich Zinoviev, Monitoring the Activity of Feed Enzymes in Vitro and Their Activity in the System that Modulates the GIT, Ecology and Evolutionary Biology. Vol. 4, No. 3, 2019, pp. 33-38. doi: 10.11648/j.eeb.20190403.12
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