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Impacts of Grazing by Milkfish (Chanos chanos Forsskal) on Periphyton Growth and its Nutritional Quality in Inland Saline Ground Water : Fish Growth and Pond Ecology
Ecology and Evolutionary Biology
Volume 1, Issue 3, December 2016, Pages: 41-52
Received: Sep. 12, 2016; Accepted: Sep. 29, 2016; Published: Nov. 3, 2016
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Sudhir Krishan Garg, Department of Zoology, CCS Haryana Agricultural University, Hisar India
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Two experiments (I and II) each under three different salinity levels (10, 15 and 20 ppt) were conducted to investigate the effects of addition of milkfish and substrate for periphyton development and its nutitional quality. Efforts were also made to investigate the hydrobiological parameters and growth of milkfish. Both experiments were run simultaneously with the difference that in experiment I, ponds were stocked with milkfish, while no fish were stocked in experiment II. No significant differences were observed in TDS levels among the three salinity treatments. Studies have revealed higher values of productivity indicating parameters (Alkalinity, NPP and GPP) under grazed conditions in comparison with the ungrazed conditions. A comparison of physico-chemical characteristics of pond water in between the two experiments (I and II) indicated not many variations. All parameters followed a trend similar to the ponds stocked with milkfish, except that BOD5 values were slightly higher and DO levels were slightly lower under ungrazed conditions. SO4 and o-PO4 levels were similar in both the trials. Addition of fish only slightly affected inorganic N-species (NO3N, NO2N), however, NH4N levels were significantly (P<0.05) low, while Alkalinity and total Kjeldahl nitrogen were significantly (P<0.05) high in treatment with fish at 15 ppt salinity. Irrespective of the water salinity, mean periphyton density scraped from the substrate increased with an increase in depth upto 50 cm in both the trials. A comparison of periphyton production/biomass and its pigment concentrations indicated significantly (P<0.05) higher values for dry matter, ash free dry matter (AFDM), ash, ash % of dry matter, algal constitutes, autotrophic index in ponds with fish (grazed conditions). On the other hand, periphyton number (units cm-2), total pigment concentration, chlorophyll a and pheophytin a remained significantly (P<0.05) higher in ponds without fish (ungrazed conditions). Results have also revealed a significant effect of salinity on fish growth with significantly (P<0.05) higher growth occurring in ponds maintained at 15 ppt salinity. Fish carcass protein, fat and phosphorus, VSI and HSI values also coincided well with highest fish growth at 15 ppt salinity. Proximate composition of periphyton had revealed significantly (P<0.05) higher nutritive value of samples obtained from ponds without fish.
Milkfish, Inland Saline Water, Growth, Periphyton, Water Quality, Grazed and Ungrazed, Nutritioal Quality of Periphyton
To cite this article
Sudhir Krishan Garg, Impacts of Grazing by Milkfish (Chanos chanos Forsskal) on Periphyton Growth and its Nutritional Quality in Inland Saline Ground Water : Fish Growth and Pond Ecology, Ecology and Evolutionary Biology. Vol. 1, No. 3, 2016, pp. 41-52. doi: 10.11648/j.eeb.20160103.11
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Abwao J A, Boera PN, Munguti JM, Orina PS and Ogello EO (2014) The potential of periphyton based aquaculture for Nile tilapia (Oreochromis niloticus L.) production. a review. International Journal of Fisheries and Aquatic Studies 2(1) 147-152.
Amisah S, Adjei-boateng D, Afianu, DD (2008) Effects of bamboo substrate and supplementary feed on growth and production of the African catfish, Clarias gariepinus. J. Appl. Sci. Environ. Manage. 12(2) 25 - 28
AOAC (Association of Official Analytical Chemists) (2016) Official Methods of Analysis. Assoc. of Off. Anal. Chem. Inc., Arlington, USA, p.684.
APHA (2005) Standard method for the estimation of water and wastewater. American Public Health Association. American waste water association and water pollution control federation. 21st ed. Washington DC.
Azim ME, Wahab MA, van Dam AA, Beveridge MCM and Verdegem MCJ (2001a) The potential of periphyton-based culture of two Indian major carps, rohu Labeo rohita (Hamilton) and gonia Labeo gonius (Linnaeus). Aquacult. Res. 32: 209-216.
Azim ME, Wahab MA, van Dam AA, Beveridge MCM, Huisman EA and Verdegem, MCJ (2001b) Optimization of stocking ratio of two Indian major carps rohu (Labeo rohita Ham.) and catla (Catla catla Ham.) in a periphyton based aquaculture system. Aquaculture 203: 33-49.
Azim ME, Verdegem MCJ, Khatoon H, Wahab M.A, van Dam AA. and Beveridge, MCM (2002) A comparison of fertilization, feeding and three periphyton substrates for increasing fish production in freshwater pond aquaculture in Bangladesh. Aquaculture 212: 227-243.
Bellinger EG (1992) A key to common Algae. The institute of water and environment management, Aquaculture. Chapman and Hall, London.P 319
Dempster PW, Beveridge MCM and Baird DJ (1993) Herbivory in tilapia Oreochromis niloticus (L.): a comparison of feeding rates on periphytonand phytoplankton. J. Fish Biol. 43: 385-392
Dempster PW, Baird DJ, Beveridge MCM (1995) Can fish survive by filter-feeding on microparticles? Energy balance in tilapia grazing on algal suspensions. J Fish Biol 47: 7-17.
Garg, S. K (1996) Brackish water carp culture in potentially waterlogged areas using animal wastes as pond fertilizers, Aquaculture International, 4: 143-155.
Garg S. K (2005) Role of periphyton in development of sustainable aquaculture technology for inland saline groundwater: A review, Indian J. Animal Sci. 75(11):1348-1353
Garg, S. K. and Bhatnagar, A. (1996) Effect of varying doses of organic and inorganic fertilizers on plankton production and fish biomass in brackish water fish ponds, Aquaculture Research, 27:157-166
Garg, S. K. and Bhatnagar,S (2016). Influence of periphyton substrate density on hydrobiological characteristics and growth performance of Nile tilapia, Oreochromis niloticus (linnaeus.1758) stocked in inland saline groundwater ponds. International Journal of Fisheries and aquatic studies. 4:444-452.
Garg S. K, Jana S. N. and Arasu, A. R. T., (2006) Determination of fertilization rate for optimum pond productivity and fish growth in inland saline groundwater ponds: Monoculture of grey mullet and milkfish. Asian Fish Sci., 19: 165-176。
Garg, S. K., Kumar, A., Arasu, A. R. T., Bhatnagar, A., Jana, S. N. and Barman, U. K., (2007) Effect of periphyton and supplementary feeding on growth performance and some aspects of nutritive physiology of Nile Tilapia, Oreochromis niloticus and Pearlspot, Etroplus suratensis under polyculture, J. Applied Aquaculture, 19(3): 19-45
Garg S. K, Barman UK and Bhatnagar A (2013) Optimization of fertilization rate for maximizing periphyton production on additional substrate and growth performance of milkfish, Chanos chanos (Forsskal) in stagnant inland saline ground water ponds. Journal of Nature Science and Sustainable Technology.7(2):1-15
Hem S and Avit JLB. (1994) First results on “acadjas enclos” as an extensive aquaculture system (West Africa). Bulletin of Marine Sci. 55: 1038-1049.
Henken AM, Lucas H, Tijssen PAT, and. Machiels MAM (1986) A comparison between methods used to determine the energy content of feed, fish and faeces samples. Aquaculture, 58: 195-201.
Huchette SMH, Beveridge MCM, Barid DJ and Ireland M (2000) The impact of grazing by tilapias (Oreochromis niloticus L.) on periphyton communities growing on artificial substrate in cages. Aquaculture.186: 45-60.
Horn MH (1989) Biology of marine herbivorous fishes. Oceanogr. Mar Biol. Ann. Rev. 27: 167-272.
Jana S.N, Garg SK and Patra BC (2004) Effect of periphyton on growth performance of grey mullet, Mugil cephalus (Linn.) in inland saline groundwater ponds.j.Appl. Ichthyol. 20 (2): 10-17.
Jana S. N, Garg SK, Arasu ART, Bhatnagar A, Kalla A and Patra BC (2006a) Use of additional substrate to enhance growth performance of milkfish, Chanos chanos (forsskal) in inland saline groundwater ponds. J. Applied Aquacult., 18(1): 1-20.
Jana SN, Garg SK and Patra BC (2006b) Effect of inland water salinity on growth performance and nutritional physiology in growing milkfish, Chanos chanos (Forsskal): field and laboratory studies. J. appl. Ichthyol. 22: 25-34.
Keshavanath P, Gangadhar B, Ramesh TJ, van Rooij JM, Beveridge MCM, Baird DJ,Verdegem, M. C. J. and van Dam, A. A. (2001) Use of artificial substrates to enhance production of freshwater herbivorous fish in pond culture. Aquacult. Res. 32: 189-197.
Keshavanath P, Gangadhar B, Ramesha TJ, Priyadarshini M, van Dam AA, Verdegem, MCJ and Beveridge MCM (2015) Impact of substrates and fish stocking density on growth and production of the Indian major carp, labeo rohita (ham.). J Aqua Trop. 30(1-2):1-14.
Kumar A, Bhatnagar A and Garg S. K (2009a) Growth performance carcass composition and digestive enzyme activity of pearlspot, Etroplus suratensis (Bloch) reared in inland saline groundwater ponds providing substrate or feed.,Live stock production for rural development, 21(10), #180. pp 1-20.
Kumar A,Bhatnagar A, Garg S K and Jana SN (2009b) Growth performance of Nile Tilapia,Oreochromis niloticus (Linn) in relation to provision of substrate and supplementary feeding and grown in brackish water ponds. Asian Fish Science, 22 (4): 1071-1100.
Konan-Brou, A. A. and Guiral, D. (1994) Available algal biomass in tropical brackishwater artificial habitats. Aquaculture 119: 175-190
Milstein A, Joseph D, Peretz Y and Harpaz S (2005) Evaluation of organic tilapia culture in periphyton-based ponds. The Israeli Journal of Aquaculture – Bamidgeh 57(3): 143-155
Milstein A, Peretz Y, Harpaz S (2009) Culture of organic tilapia to market size in periphyton-based ponds with reduced feed inputs. Aquac. Res. 40, 55-59.
Nayar S and Gowda G. (1999) Phytobenthic production during bloom phase in a tropical coastal lagoon near Mangalore. Indian J. Environ. Sci. 3: 69-73.
Prescott GW (1962) Algae of the Western Great Lake area. Wm. C. Brown. Co., Dubuque, IA.
Prejs A (1984) Herbivory by temperate freshwater fishes and its consequences. Environmental Biology of Fishes. 10: 281-296.
Ramesh MR, Shankar KM, Mohan CV and Varghese TJ (1999) Comparison of three plant substrates for enhancing carp growth through bacterial biofilm. Aquacult. Eng. 19: 119-131.
Shilta M T, Chadha N K, Pandey P K,Sawant P B.(2015) Effect of biofilm on water quality and growth of Etroplus suratensis (Bloch, 1790). Aquacult.Int. (in press).
Snedecor G W and Cochran W C (1982) Statistical methods. Iowa State University Press, Ames. 1A, pp 507
van Dam AA, Beveridge MCM, Azim ME. (2002) The potential of fish production based on periphyto Rev. Fish Biol. Fish, 12:1–31.
Ward HB and GC Whipple (1959) Freshwater Biology, John Wiley and Sons, pp. 1248.
Washington HG (1984) Diversity, biotic and similarity indices. Water Res. 18 (6): 653-694.
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