Sensitivity of Redox Cycle Enzymes in Substantiating the Pathophysiology of Cataract
International Journal of Ophthalmology & Visual Science
Volume 2, Issue 1, February 2017, Pages: 15-21
Received: Feb. 2, 2017; Accepted: Feb. 27, 2017; Published: Mar. 15, 2017
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Syed Parween Ali, Department Medical Laboratory Science, College of Applied Medical Sciences, King Khalid University, Abha, Kingdom of Saudi Arabia
Mohammed Abdul Rasheed, Ministry of Interior, Abha, Kingdom of Saudi Arabia
Mohammed Amanullah, Department of Biochemistry, College of Medicine, King Khalid University, Abha, Kingdom of Saudi Arabia
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Oxidative modifications play major role in the formation of cataract. Lens contains several protective mechanisms against oxidizing agents viz. catalase, superoxide dismutase, glutathione reductase, glutathione peroxidase, and ascorbic acid. To explore the oxidative damage that might be occurring with 'Invitro' development of cataracts induced by sugars, H2O2 and steroids. We examined redox status of cataractous lenses by analysing enzymatic defence meachanisms. Lenses were exposed to glucose (50 mM) (Group – I); galactose (35 mM) (Group – II) and xylose (30 mM) (Group – III) and maintained at 37°C for 72 hours so as to induce sugar cataract. H2O2 cataract was produced by adding 50 mM (Group – IV) and 100 mM (Group – V), solution to culture media (AAH). Steroid cataract was generated by adding a freshly prepared l x 10-4 M dexamethasone (mw 392.5) (Group – VI) in absolute alcohol to the culture media (AAH) and incubated at 37°C for 72 hours. Subsequent to the development of the cataract, the lenses were homogenized and the specific activity of the enzymes catalase (CAT), glutathione peroxidase and glutathione reductase was assessed. Catalase activity did not show any significant decrease in sugar cataract and steroid cataract but a significant decrease was observed in H2O2 cataract. However a significant decrease in GSH-Px and GSH-Rx were found in all the three types of experimental cataract as compared to control lenses.
Cataract, Catalase, Glutathione Reductase, Glutathione Peroxidase, Free Radicals
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Syed Parween Ali, Mohammed Abdul Rasheed, Mohammed Amanullah, Sensitivity of Redox Cycle Enzymes in Substantiating the Pathophysiology of Cataract, International Journal of Ophthalmology & Visual Science. Vol. 2, No. 1, 2017, pp. 15-21. doi: 10.11648/j.ijovs.20170201.14
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