LC/MS Analysis of Cyclo Fatty Acid-containing Triacylglycerols in Cottonseed Oil
Science Journal of Analytical Chemistry
Volume 8, Issue 1, March 2020, Pages: 12-17
Received: Dec. 22, 2019; Accepted: Jan. 15, 2020; Published: Jan. 31, 2020
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Shuji Hirayama, Biomaterial in Tokyo Co., Ltd., Tokyo, Japan
Daichi Shinozaki, Biomaterial in Tokyo Co., Ltd., Tokyo, Japan
Yoshiya Izumi, Biomaterial in Tokyo Co., Ltd., Tokyo, Japan
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We measured the constituent triacylglycerol of cotton oil, the only edible oil containing a cyclo fatty acid. Due to the difficulty of obtaining a standard for triacylglycerols containing cyclo fatty acids, cotton oil is analyzed using cyclo triacylglycerol in kapok oil as a reference. Analytical methods used liquid chromatography fast atom bombardment mass spectrometry (LC / FAB-MS) using a matrix of 3% m-NBA containing 3 mM NaCl, and orthogonal transfer method for APCI / TOF-MS. FAB mass spectral properties of triacylglycerols containing cyclo fatty acids can be observed with [M + Na]+, [M-1]+ molecular ions, and [M- (RCOO)] + fragments. In the orthogonal APCI / TOF-MS spectrum, only the [M-1] + molecular ion appears, so that the presence of triacylglycerol containing CPFA becomes apparent. On the other hand, chain triacylglycerol can be observed as [M + 1]+ molecular ions and [M- (RCOO)]+ fragments. Based on these observations, triacylglycerols containing ten types of cyclo fatty acids were successfully successfully detected in crude cottonseed oil. In addition, the APCI/TOF-MS technique successfully detected dilinoleoyl-marvalyl-glycerol and palmtoyl-linoleoyl-marvalyl glycerol in the purified cottonseed oil.
LC/FAB-MS, Cyclo Fatty Acid, Kapok Oil, Cottonseed Oil
To cite this article
Shuji Hirayama, Daichi Shinozaki, Yoshiya Izumi, LC/MS Analysis of Cyclo Fatty Acid-containing Triacylglycerols in Cottonseed Oil, Science Journal of Analytical Chemistry. Vol. 8, No. 1, 2020, pp. 12-17. doi: 10.11648/j.sjac.20200801.13
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