Comparative Voltammetric Behavior and Determination of Mephenesin in Pure Form, Pharmaceuticals and Biological Fluids at Pencil Graphite and Glassy Carbon Electrodes
Science Journal of Analytical Chemistry
Volume 7, Issue 2, March 2019, Pages: 32-41
Received: Feb. 28, 2019; Accepted: Apr. 3, 2019; Published: May 7, 2019
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Ehab El-Kady, Pharmaceutical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt
Hoda El-Qudaby, National Organization for Drug Control and Research (NODCAR), Giza, Egypt
Marwa Omran, National Organization for Drug Control and Research (NODCAR), Giza, Egypt
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New voltammetric methods are introduced for the determination of a skeletal muscle relaxant namely; mephenesin (Mep) in its pure form with the application in the pharmaceutical preparation and biological fluids. Three voltammetric methods namely; cyclic voltammetry (CV), differential pulse voltammetry (DPV) and square wave voltammetry (SWV) using pencil graphite electrode (PGE) and glassy carbon electrode (GCE) were used. Voltammetric behavior of Mep was investigated in Britton Robinson (BR) buffer as electrolyte in pH range 2-10. CV produced one irreversible anodic peak revealing oxidation reaction in pH 6 as maximum pH value at 1.33 and 1.36 V using PGE and GCE, respectively. At PGE, DPV and SWV were investigated and linearity ranges were 18.02-119.07 and 18.02-70.0 µg/mL with correlation coefficient 0.9997 and 0.9995, LOD values were found to be 0.113 and 0.059 µg/mL, respectively. At GCE, linearity ranges were 9.05-44.39 and 4.54-65.78 µg/mL with correlation coefficient 0.9996 and 0.9999, LOD values were 0.553 and 0.865 µg/mL at DPV and SWV, respectively. The proposed methods were applied with good recovery in pharmaceutical preparation, human mother milk and urine. They were validated and revealed accurate and precise results.
Mephenesin, Pharmaceuticals, Biological Fluids, Glassy Carbon Electrode, Pencil Graphite Electrode, Voltammetry
To cite this article
Ehab El-Kady, Hoda El-Qudaby, Marwa Omran, Comparative Voltammetric Behavior and Determination of Mephenesin in Pure Form, Pharmaceuticals and Biological Fluids at Pencil Graphite and Glassy Carbon Electrodes, Science Journal of Analytical Chemistry. Vol. 7, No. 2, 2019, pp. 32-41. doi: 10.11648/j.sjac.20190702.11
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A. Brayfield., The complete drug reference, Martindale. 38th ed. Vol. A. (2014), London: Pharmaceutical Press.
G. Frederick Hofmann, S. Walter Root and M. Cedric Smith, The Nervous System: Central Nervous System Drugs, 2 (1965) 14-28.
P. Guinebault, C. Colafranceschi and G. Bianchetti, Journal of Chromatography A, 507 (1990) 221-225.
S. V. Mulgund, M. S. Phoujdar, S. V. Londhe, P. S. Mallade, T. S. Kulkami, A. S. Deshpande and K. S. Jain, Indian Journal of Pharmaceutical Sciences, 71(2009) 35–40.
M. B. Devani, C. J. Shishoo, B. N. Suhagia and S. A. Shah, Indian Journal of Pharmaceutical Sciences, 56 (1994) 41-44.
A. R. Maass, P. L. Carey and A. E. Heming, Journal of Analytical chemistry, 31 (1959) 1331–1334.
A. M. Gillespie and S. M. Walters, Journal of Analytical letters, 6 (1973) 61-69.
F. Sayin and S. Kir, Journal of Pharmaceutical Biomedical Analysis, 25 (2001) 153–163.
S. A. Ö zkan, B. Uslu and P. uman, Analytical Chim. Acta., 457 (2002) 265–274.
B. Uslu and S. A. Özkan, Electrochimica acta, 49 (2004) 4321-4329.
J.-M. Kauffmann and J.-C. Vire, Analytica chimica acta, 273, 1-2, (1993) 329-334.
N. J. Ronkainen, H. B. Halsall and W. R. Heineman, Chemical Society Reviews, 39(2010) 1747-1763.
J. Wang, John Wiley & Sons, 1988.
S. A. Özkan, B. Uslu and Z. Sentürk, Electroanalysis, 16, 3 (2004) 231-237.
M. A. Koda-Kimble and L. Y. Young, Applied Therapeutics: Clinical Use of Drugs vol. 1, 5th ed., (1992) 18-5.‏
R. Ouyang, Z. Zhu, C. E. Tatum, J. Q. Chambers and Z.-L. Xue, Journal of Electroanalytical Chemistry, 656 (2011) 78-84.
E. Dede, Ö. Saglam and Y. Dilgin, Electrochimica Acta, 127(2014) 20–26.
A. Levent, Y. Yardim and Z. Senturk, Electrochimica Acta, 55, 1(2009)190–195.
W. Gao, J. Song, and N. Wu, Journal of Electroanalytical Chemistry, 576, 1(2005)1–7.
D. Demetriades, A. Economou, and A. Voulgaropoulos, Analytica Chimica Acta, 519, 2(2004) 167–172.
H. Karadeniz, B. Gulmez, F. Sahinci et al, Journal of Pharmaceutical and Biomedical Analysis, 33, 2 (2003) 295–302.
A. M. Bond, P. J. Mahon, J. Schiewe, and V. Vicente-Beckett, Analytical Chemistry Acta, 345, 1–3, (1997) 67–74.
M. Rizk, H. A. Hendawy, M. M. A. El-Alamin and M. I. Moawad, Journal of Electroanalytical Chemistry, 749 (2015) 53-61.
M. Rizk, M. M. Abou El-Alamin, H. A. Hendawy and M. I. Moawad, Electroanalysis, (2015).
H. T. S. Britton and R. A. Robinson, Journal of the Chemical Society (Resumed), (1931) 1456-1462.
J. Heyrovsky and P. Zuman, Practical Polarography, Academic Press, New York, (1968) 163-179.
S. Shen, H. Shi and H. Sun, International Journal of Chemical Kinetics, 39 (2007) 440-446.
E. Laviron, Journal of Electroanalytical Chemistry, 101(1979), 19–28.
H. Elqudaby, G. G. Mohamed and G. M. El Din, International Journal of Electrochemical Sciences, 9 (2014) 856-869.
A. Radi and Z. El-Sherif, Talanta 58 (2002) 319–324.
B. Dogan-Topal, B. Bozal-Palabıyık, B. Uslu and S. A. Ozkan, Sensors Actuators B Chemical, 177 (2013) 841–847.
A. Shalaby, W. S. Hassan, H. A. Hendawy and A. Ibrahim, Journal of Electroanalytical Chemistry, 763 (2016) 51-62.
M. A. El-ries, G. G. Mohamed and A. K. Attia, YAKUGAKU ZASSHI, the pharmaceutical society of Japan, 128(2008) 171-177.
D. K. Gosser, Cyclic Voltammetry: Simulation and Analysis of Reaction Mechanisms, VCH, New York, N. Y., 1993.
A. J. Bard and L. R. Faulkner, Elecrochemical Techniques Fundamentals and Applications, second ed. John Wiley and Sons, New York, 2001.
L. Agüı, A. Guzmán, P. Yáñez-Sedeño and J. Pingarrón, Analytica Chimica Acta, 461 (2002) 65–73.
C. M. Riley and T. W. Rosanske, Development and validation of analytical techniques, Elsevier, 1996.
B. Uslu and S. A. Özkan, Analytica Chimica Acta, 462 (2002) 49-57.
B. Dogan-Topal, B. Uslu and S. A. Özkan, Sensors and Actuators B: Chemical, 177 (2013) 841-847.
M. E. Swartz and I. S. Krull, Handbook of analytical validation, CRC Press, 2012.
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