Determination of Essential and Non-essential Metals Concentration in Garlic (Allium sativum L.) Bulb and Leaf Cultivated in Ambo Woreda, Ethiopia
Science Journal of Analytical Chemistry
Volume 4, Issue 6, November 2016, Pages: 84-94
Received: May 23, 2016; Accepted: Jun. 2, 2016; Published: Jan. 3, 2017
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Authors
Wodaje Addis Tegegne, Department of Chemistry, College of Natural and Computational Science, Ambo University, Ambo, Ethiopia
Alemayehu Abebaw Mengiste, Department of Chemistry, College of Natural and Computational Science, Ambo University, Ambo, Ethiopia
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Abstract
In this study, the levels of twelve essential metals (Na, K, Ca, Mg, Fe, Zn, Mn, Cu, Mo, Co, Cr and Ni) and two non-essential metals (Pb and Cd) were determined in the bulb and leafs of garlic (Allium sativum L.) cultivated in Ambo Woreda, Ethiopia. Wet digestion method using a mixture of 5 ml of concentrated HNO3:HClO4 (4:1 v/v) was used for digestion of the samples. The determination processes were done by flame photometer for Na and K, EDTA titration method for Ca and Mg, and ICP-OES for the rest of the metals. The results obtained revealed that the concentrations of metals in the garlic bulb samples in mg/kg dry weight were in the range of: Na (217–366.7), K (9080–12060), Ca (1018–1286), Mg (802–992.6), Fe (63.44–91.24), Zn (31.17–35.39), Mn (5.27–7.51), Cu (4.21–7.16), Mo (1.06–2.08), Co (0.61–1.49), Ni (1.45–3.78), Cr (0.47–1.31), Pb (1.07–2.51) and Cd (0.10–0.16). The concentrations of metals in the garlic leaf samples in mg/kg dry weight were in the range of: Na (463–730), K (11370–12860), Ca (1209–1302), Mg (871–994), Fe (72.3–108), Zn (49.1–71.39), Mn (26.74–72.36), Cu (5.41–8.44), Mo (1.01–2.30), Co (1.17–4.96), Ni (2.17–3.54), Cr (1.20–2.17), Pb (1.87–2.84) and Cd (0.12–0.18). In addition, the results show that the levels of elements were higher in the leaves than the bulbs. In general, the levels of metals in the analyzed garlic bulb and leaf samples were found below the FAO/WHO maximum permissible limit; hence they are safe for human consumption and can be considered as a good source of essential nutrients.
Keywords
Essential Metals, Non-essential Metals, Garlic (Allium sativum L.), Wet Digestion, ICP-OES
To cite this article
Wodaje Addis Tegegne, Alemayehu Abebaw Mengiste, Determination of Essential and Non-essential Metals Concentration in Garlic (Allium sativum L.) Bulb and Leaf Cultivated in Ambo Woreda, Ethiopia, Science Journal of Analytical Chemistry. Vol. 4, No. 6, 2016, pp. 84-94. doi: 10.11648/j.sjac.20160406.12
Copyright
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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