Science Journal of Analytical Chemistry
Volume 4, Issue 6, November 2016, Pages: 84-94
Received: May 23, 2016;
Accepted: Jun. 2, 2016;
Published: Jan. 3, 2017
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Wodaje Addis Tegegne, Department of Chemistry, College of Natural and Computational Science, Ambo University, Ambo, Ethiopia
Alemayehu Abebaw Mengiste, Department of Chemistry, College of Natural and Computational Science, Ambo University, Ambo, Ethiopia
In this study, the levels of twelve essential metals (Na, K, Ca, Mg, Fe, Zn, Mn, Cu, Mo, Co, Cr and Ni) and two non-essential metals (Pb and Cd) were determined in the bulb and leafs of garlic (Allium sativum L.) cultivated in Ambo Woreda, Ethiopia. Wet digestion method using a mixture of 5 ml of concentrated HNO3:HClO4 (4:1 v/v) was used for digestion of the samples. The determination processes were done by flame photometer for Na and K, EDTA titration method for Ca and Mg, and ICP-OES for the rest of the metals. The results obtained revealed that the concentrations of metals in the garlic bulb samples in mg/kg dry weight were in the range of: Na (217–366.7), K (9080–12060), Ca (1018–1286), Mg (802–992.6), Fe (63.44–91.24), Zn (31.17–35.39), Mn (5.27–7.51), Cu (4.21–7.16), Mo (1.06–2.08), Co (0.61–1.49), Ni (1.45–3.78), Cr (0.47–1.31), Pb (1.07–2.51) and Cd (0.10–0.16). The concentrations of metals in the garlic leaf samples in mg/kg dry weight were in the range of: Na (463–730), K (11370–12860), Ca (1209–1302), Mg (871–994), Fe (72.3–108), Zn (49.1–71.39), Mn (26.74–72.36), Cu (5.41–8.44), Mo (1.01–2.30), Co (1.17–4.96), Ni (2.17–3.54), Cr (1.20–2.17), Pb (1.87–2.84) and Cd (0.12–0.18). In addition, the results show that the levels of elements were higher in the leaves than the bulbs. In general, the levels of metals in the analyzed garlic bulb and leaf samples were found below the FAO/WHO maximum permissible limit; hence they are safe for human consumption and can be considered as a good source of essential nutrients.
Wodaje Addis Tegegne,
Alemayehu Abebaw Mengiste,
Determination of Essential and Non-essential Metals Concentration in Garlic (Allium sativum L.) Bulb and Leaf Cultivated in Ambo Woreda, Ethiopia, Science Journal of Analytical Chemistry.
Vol. 4, No. 6,
2016, pp. 84-94.
Kamenetsky, R., Faigenboim, A., Mayer, E. S., Michael, T. B., Gershberg, C., Kimhi, S., Esquira, I., Shalom, S. R., Eshel, D., Rabinowitch, H. D. and Sherman, A. (2015). Integrated transcriptome catalogue and organ-specific profiling of gene expression in fertile garlic (Allium sativum L.). BMC Genomics, 16 (12), 1-15.
Kallel, F., Driss, D., Chaari, F., Belghith, L., Bouaziz, F., Ghorbel, R. and Chaabouni, S. E. (2014). Garlic (Allium sativum L.) husk waste as a potential source of phenolic compounds: Influence of extracting solvents on its antimicrobial and antioxidant properties, Industrial Crops and Products, 62, 34-41.
Diriba, S. G., Kebede, W., Nigussie, D. R., Getachew, T. and Sharma, J. J. (2013). Postharvest quality and shelf life of garlic bulb as influenced by storage season, soil type and different compound fertilizers. Journal of Postharvest Technology, 1 (1), 69-83.
Deresse, D. (2010). Antibacterial effect of garlic (Allium sativum) on Staphylococcu aureus: An in vitro study. Asian Journal of Medical Sciences, 2 (2), 62-65.
Packia Lekshmi, N. C. J., Viveka, S., Jeeva, S. and Raja Brindha, J. (2015). Antimicrobial Spectrum of Allium Species: A Review. Indian journal of Science, 15 (44), 1–5.
Adekunle, I. M., Olorundare, O. and Nwange, C. (2009). Assessments of lead levels and daily intakes from green leafy vegetables of southwest Nigeria. Nutrition and Food Science, 39 (4), 413-422.
Hellen, L. E., & Othman, O. C. (2014). Levels of selected heavy metals in soil, tomatoes and selected vegetables from Lushoto district-Tanzania. International Journal of Environmental Monitoring and Analysis, 2 (6), 313-319.
Kachenkom, A. G. and Singh, B. (2006). Heavy metal contamination in vegetables grown in urban and metal smelter contaminated sites in Australia. Water, Air and Soil Pollution, 169 (1-4), 101-123.
Wilson, B. and Pyatt, F. B. (2007). Heavy metal dispersion, persistence, and bioaccumulation around an ancient copper mine situated in Anglesey, UK. Ecotoxicology and Environmental Safety, 66, 224-231.
Tasrina, R. C., Rowshon, A., Mustafizur, A. M. R., Rafiqul, I. and Ali., M. P. (2015). Heavy metals contamination in vegetables and its growing soil. Journal of Environmental Analytical Chemistry, 2 (3), 1-6.
Chailapakul, O., Korsrisakul, S., Siangroh, W. and Grudpan, K. (2007). Fast and simultaneous detection of heavy metals using a simple reliable microchip-electrochemistry route: An alternative approach to food analysis. Talanta, 74, 683-689.
Hamza, N. A. E., Hammad, A.Y. and Eltayeb, M.A. (2013). Adsorption of Metals (Fe(II), Cr(III) and Co(II)) from aqueous solution by using Activated carbon prepared from Mesquite tree. Science Journal of Analytical Chemistry, 1(2), 12-20.
Dibofori-Orji, A. N. and Edori, O. S. (2015). Analysis of some heavy metals (Pb, Cd, Cr, Fe, Zn) in processed cassava flour (garri) sold along the road side of a busy highway. Archives of Applied Science Research, 7 (2), 15-19.
Kassa, B. and Hailay, K. (2014). Spectroscopic determination of trace metals (Mn, Cu and Ni) content in Moringa oleifera. International Journal of chemical and Natural Sciences, 2 (5), 141-144.
Chauhan, A., Mittu, B. and Chauhan, P. (2015). Analytical method development and validation: A concise review. Journal of Analytical and Bioanalytical Techniques, 6 (1), 1-5.
Iqbal, J., Carney, W. A., LaCaze, S. and Theegala, C. S. (2010). Metals determination in biodiesel (B100) by ICP-OES with microwave assisted acid digestion. The Open Analytical Chemistry Journal, 4, 18-26.
Kiflom, G. and Tarekegn, B. (2015). Determination of some selected heavy metals in fish and water samples from Hawassa and Ziway Lakes. Science Journal of Analytical Chemistry, 3(1), 10-16.
Shrivastava, A. and Gupta, V. B. (2011). Methods for the determination of limit of detection and limit of quantitation of the analytical methods: Review Article. Chronicles of Young Scientists, 2 (1), 21-25.
Thomas A. L. (2015). Method validation essentials, limit of blank, limit of detection, and limit of quantitation. Bio Pharma International, 28 (4), 48–51.
Mitra, S. (2003). Sample preparation techniques in analytical chemistry (Vol. 162, pp. 6-244). Hoboken: John Wiley and sons, Inc.
Harvey, D. (2000). Modern analytical chemistry (1st ed., pp. 706-7110). Depauw University, United States of America: McGraw-Hill.
USEPA. (2010). National functional guidelines for inorganic superfund; Data review.USEPA-540-R10-011, Washington, DC.
FAO/WHO (Codex Alimentarius Commission). (2001). Food additives and contaminants. Joint FAO/WHO food standards program: ALINORM 01/12A: 1-289.
WHO. (1998). Quality control methods for medicinal plant materials, WHO, Geneva, Switzerland.
Umar, M. A.and Salihu, Z. O. (2014). Heavy metals content of some spices available within FCT-Abuja, Nigeria. International Journal of Agricultural and Food Science, 4 (1), 66-74.