Survey of Lysogenic Phages in the 72 Strains of Escherichia coli Collection of Reference (ECOR) and Identification of a Phage Derived from the ECOR52 Strain
American Journal of BioScience
Volume 2, Issue 2, March 2014, Pages: 32-37
Received: Jan. 29, 2014;
Published: Feb. 28, 2014
Views 2527 Downloads 114
Yuka Shibata, Graduate School of Humanities and Sciences, Nara Women’s University, Nara, Japan
Chisato Ugumori, Faculty of Human Life and Environment, Nara Women’s University, Nara, Japan
Anna Takahashi, Faculty of Human Life and Environment, Nara Women’s University, Nara, Japan
Ayuka Sekoguchi, Graduate School of Humanities and Sciences, Nara Women’s University, Nara, Japan
Sumio Maeda, Graduate School of Humanities and Sciences, Nara Women’s University, Nara, Japan;Faculty of Human Life and Environment, Nara Women’s University, Nara, Japan
Escherichia coli collection of reference (ECOR) is a standard collection of 72 wild-type E. coli strains that represent natural E. coli populations found in various environments. Although these strains are widely used in experiments investigating the physiology and behavior of wild-type E. coli, their genetic features including accessory DNA have not been sufficiently studied. In this study, we surveyed for the presence of lysogenic phages in each ECOR strain under both inducing and non-inducing conditions. We found that 34 strains could produce plaque-forming phages; among them, 14 strains were newly discovered to harbor lysogenic phages capable of entering the lytic cycle. We isolated a new phage (designated as “MSU52-L1”) from the ECOR52 strain and identified it as a P22/lambda-like phage with homology to known phages, such as CUS-3, HK620, and HK140.
Survey of Lysogenic Phages in the 72 Strains of Escherichia coli Collection of Reference (ECOR) and Identification of a Phage Derived from the ECOR52 Strain, American Journal of BioScience.
Vol. 2, No. 2,
2014, pp. 32-37.
F. R. Blattner, G. Plunkett 3rd, C. A. Bloch, N. T. Perna, V. Burland, M. Riley, J. Collado-Vides, J. D. Glasner, C. K. Rode, G. F. Mayhew, J. Gregor, N. W. Davis, H. A. Kirkpatrick, M. A. Goeden, D. J. Rose, B. Mau, and Y. Shao, "The complete genome sequence of Escherichia coli K-12," Science, vol. 277, pp. 1453–1462, 1997.
H. Ochman and R. K. Selander, "Standard reference strains of Escherichia coli from natural populations," J Bacteriol, vol. 157, pp. 690–693, 1984.
O. Tenaillon, D. Skurnik, B. Picard, and E. Denamur, "The population genetics of commensal Escherichia coli," Nat Rev Microbiol, vol. 8, pp. 207-217, 2010.
A. Hurtado and F. Rodríguez-Valera, "Accessory DNA in the genomes of representatives of the Escherichia coli reference collection," J Bacteriol, vol. 181, pp. 2548-2554, 1999.
E. J. Battaglioli, G. A. Baisa, A. E. Weeks, R. A. Schroll, A. J. Hryckowian, and R. A. Welch, "Isolation of generalized transducing bacteriophages for uropathogenic strains of Escherichia coli," Appl Environ Microbiol, vol. 77, pp. 6630-6635, 2011.
L. E. Williams, J. Wireman, V. C. Hilliard, and A. O. Summers, "Large plasmids of Escherichia coli and Salmonella encode highly diverse arrays of accessory genes on common replicon families," Plasmid, vol.69, pp. 36-48, 2013.
M. A. Riley and D. M. Gordon, "A survey of Col plasmids in natural isolates of Escherichia coli and an investigation into the stability of Col-plasmid lineages," J Gen Microbiol, vol. 138, pp. 1345-1352, 1992.
A. S. Nilsson, J. L. Karlsson, and E. Haggård-Ljungquist, "Site-specific recombination links the evolution of P2-like coliphages and pathogenic enterobacteria," Mol Biol Evol, vol. 21, pp. 1-13, 2004.
C. H. Sandt, J. E. Hopper, and C. W. Hill, "Activation of prophage eib genes for immunoglobulin-binding proteins by genes from the IbrAB genetic island of Escherichia coli ECOR-9," J Bacteriol, vol. 184, pp. 3640-3648, 2002.
B. J. Bachmann, "Derivations and genotypes of some mutant derivatives of Escherichia coli K-12," In: F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low Jr, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, H. E. Umbarger Eds. Escherichia coli and Salmonella typhimurium Cellular and Molecular Biology. 2nd ed. Washington, D.C., ASM Press. 1996; 2460-2488.
S. Takeshita, M. Sato, M. Toba, and W. Masahashi, "Hashimoto-Gotoh T. High-copy-number and low-copy-number plasmid vectors for lacZ α-complementation and chloramphenicol-or kanamycin-resistance selection," Gene, vol. 61, pp. 63-74, 1987.
C. Yanisch-Perron, J. Vieira, and J. Messing. "Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors," Gene, vol. 33, pp. 103-119, 1985.
D. M. Gordon, M. A. Riley, and T. Pinou, "Temporal changes in the frequency of colicinogeny in Escherichia coli from house mice," Microbiology, vol. 144, pp. 2233-2240, 1998.
T. Maniatis, E. F. Fritsch, and J. Sambrook, Molecular Cloning. Cold Spring Harbor Laboratory: New York, 1982.
M. R. King, R. P. Vimr, S. M. Steenbergen, L. Spanjaard, G. Plunkett 3rd, F. R. Blattner, and E. R. Vimr, "Escherichia coli K1-specific bacteriophage CUS-3 distribution and function in phase-variable capsular polysialic acid O acetylation," J Bacteriol, vol. 189, pp. 6447-6456, 2007.
A. J. Clark, W. Inwood, T. Cloutier, and T. S. Dhillon, "Nucleotide sequence of coliphage HK620 and the evolution of lambdoid phages" J Mol Biol, vol. 311, pp. 657-679, 2001.
P. E. Prevelige Jr, Bacteriophage P22. In: R. Calendar Ed. The Bacteriophages. 2nd ed. New York, Oxford University Press. 2006; 457-468.