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Nephrotoxic Effects of Arsenic in Albino Mice
American Journal of BioScience
Volume 4, Issue 3-1, May 2016, Pages: 1-4
Received: Nov. 24, 2015; Accepted: Jan. 3, 2016; Published: Feb. 23, 2016
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Suman Sharma, Department of Zoology & Environmental Sciences, Punjabi University, Patiala, India
Anjali Singh Gill, Department of Zoology & Environmental Sciences, Punjabi University, Patiala, India
Zorawar Singh, Department of Zoology, Khalsa College, Amritsar, Punjab, India
Charanjit Kaur, Department of Zoology & Environmental Sciences, Punjabi University, Patiala, India
P. Vijaya, Department of Zoology & Environmental Sciences, Punjabi University, Patiala, India
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Arsenic is an ubiquitous element in the environment. In the present study we investigated the effects of arsenic trioxide (AsIII) on lipid peroxidation and on the activity of antioxidant enzymes in the kidney of albino mice. Albino mice were divided into three groups. Group I were kept as control. Group II were administrated an oral dose of arsenic trioxide (3mg/kg b.w.). Group III were given an oral dose of arsenic trioxide (6mg/kg b.w.). They were acclimatized for 15 days before administration of arsenic trioxide. The autopsies were done from all the groups at 15 days post-treatment. Malondialdehyde (MDA) and activities of Superoxide dismutase (SOD) and Catalase (CAT) were analyzed in kidney of albino mice. The results showed a significant increase in concentration of MDA (p<0.05). Activities of SOD and CAT were found to decrease significantly (p<0.05). The results indicated that arsenic induced oxidative stress in albino mice by producing free radicals and lipid peroxidation where antioxidant enzymes were used as biomarkers.
Arsenic, Malondialdehyde, Lipid Peroxidation, Oxidative Stress, CAT, SOD
To cite this article
Suman Sharma, Anjali Singh Gill, Zorawar Singh, Charanjit Kaur, P. Vijaya, Nephrotoxic Effects of Arsenic in Albino Mice, American Journal of BioScience. Special Issue: Recent Trends in Experimental Toxicology. Vol. 4, No. 3-1, 2016, pp. 1-4. doi: 10.11648/j.ajbio.s.2016040301.11
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
T. S. Singh and K. K. Pant (2004) Equilibrium, kinetics and thermodynamic studies for adsorption of As (III) on activated alumina. Sep. Purif. Technol, 36: 139-147.
P. H. Patra, S. Bandyopadhyay, M. C. Bandyopadhyay, T. K. Mandal (2013) Immunotoxic a genotoxic potential of arsenic and its chemical species in goats. Toxicol. Int, 20: 6‑10.
D. Ghosh, S. Ghosh, S. Sarkar, A. Ghosh, N. Das, K. Das, Saha (2010) Quercetin in vesiculardelivery systems: Evaluation in combating arsenic‑induced acute liver toxicity associated gene expression in rat model. Chem. Biol. Interact, 186: 61‑71.
P. B, Tchounwou, A. K, Patolla, J. A, Centeno (2003) Carcinogenic and systemic health effects associated with arsenic exposure‑acritical review. Toxicol. Pathol 31: 575‑88.
P. H. Patra, S. Bandyopadhyay, R. Kumar, B. K. Datta, C. Maji, S. Biswas (2012) Quantitative imaging of arsenic and its species in goat following long term oral exposure. Food Chem. Toxicol, 50: 1946‑50.
M. F. Hughes (2002) Arsenic toxicity and potential mechanisms of action. Toxicol. Letters, 33: 1-16.
S. Singh, Z. Singh, S. S. Hundal (2015) Toxicological aspects of arsenic in different animal models: A Review. Int. J. Anal. Pharma. Biol. Sci., 4 (2): 6-15.
S. M. Keyse, R. M. Tyrell (1989) Heme oxygenase is the major 32-kDa stress protein induced in human skin fibroblasts by UVA radiation, hydrogen peroxide and sodium arsenite. Proc. Natl. Acad. Sci, USA, 86: 99-103.
T. S. Wang, H. Huang (1994) Active oxygen species are involved in the induction of micronuclei in XRS-5 cells. Mutagenesis, 9: 253-257.
S. X. Liu, M. Athar, I. Lippai, C. Waldren, T. K. Hei (2001) Induction of oxygen radicals by arsenic: implications for mechanism of genotoxicity. Proc. Natl. Acad. Sci, 98: 1643-1648.
P. Manna, M. Sinha, P. C. Sil (2008) Arsenic-induced oxidative myocardial injury: Protective role of arjunolic acid. Arch. Toxicol, 82: 137-49.
S. J. Flora, S. Chouhan, G. M. Kannan, M. Mittal, H. Swarnkar (2008) Combined administration of taurine and monoisoamyl DMSA protects arsenic induced oxidative injury in rats. Oxid. Med. Cell. Longev, 1: 39-45.
H. Naranmandura, S. Xu, S. Koike (2012) The endoplasmic reticulum is a target organelle for trivalent dimethylarsinic acid (DMA𝐼𝐼𝐼)-induced cytotoxicity. Toxicol. App. Pharmacol., 260: 241–249.
H. Naranmandura, S. Xu, T. Sawata (2011) Mitochondria are the main target organelle for trivalent monomethylarsonous acid (MMA III)-induced cytotoxicity. Chem. Res. Toxicol., 24: 1094–1103.
A. N. Chaudhuri, S. Basu, S. Chattopadhyay, S. D. Gupta (1999) Effect of high arsenic content in drinking water on rat brain. Indian. J. Biochem. Biophys, 36: 51-4.
E. O. Farombi, O. A. Adelow, Y. R. Ajimoko (2007) Biomarkers of oxidative stress and heavy metals levels as indicators of environmental pollution in African cat fish (Clarias gariepinus) from Nigeria ogun river. Int. J. Environ. Res. Public Health, 4: 158‑65.
J. M. McCord, B. B. Keele, I. Fridovich (1976) An enzyme based theory of obligate anaerobis: The physiological functions of superoxide dismutase. Proc. Natl. Acad. Sci, 68: 1024-31.
J. M. Gutteridge (1995) Lipid peroxidation and antioxidants as biomarkers of tissue damage. Clin. Chem., 41: 1819-28.
A. M. Al‑Attar (2011) Antioxidant effect of vitamin E treatment on some heavy metals – induced renal and testicular injuries in male mice. Saud. J. Biol. Sci, 18: 63‑72.
C. G. Alimba, A. A. Bakare, O. O. Aina (2012) Liver and kidney dysfunction in wistar rats exposed to municipal landfill leachate. Res. Enivron., 2: 150‑63.
A. J. Searle, R. Wilson (1980) Glutathione peroxide effect ofhydroxyl and bromine free radicals on enzyme activity. Int. J. Radiat. Biol, 37: 213-217.
J. A. Imlay, S. Linn (1988) DNA damage and oxygen radical toxicity. Sci, 240: 1302-1309.
L. Wang, Z. R. Xu, X. Y. Jia, J. F. Jiang, X. Y. Han (2006) Effects of Arsenic (AsIII) on Lipid Peroxidation, Glutathione Content and Antioxidant Enzymes in Growing Pigs. Asian-Aust. J. Anim. Sci, 19: 727-733.
Z. Singh, I. P. Karthigesu, P. Singh, R. Kaur (2014) Use of malondialdehyde as a biomarker for assessing oxidative stress in different disease pathologies: A Review. Iranian J. Pub. Health 43 (3): 7-16.
Z. Singh, P. Chadha, S. Sharma (2013) Evaluation of oxidative stress and genotoxicity in battery manufacturing workers occupationally exposed to lead. Toxicol. Int., 20 (1): 95-100.
Z. Singh, P. Chadha (2013) Oxidative stress assessment among iron industry grinders. Biochem. Cell. Arch., 12 (1): 65-68.
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