High Performance Thin Layer Chromatography Analysis of Bioactive Components from Curcuma longa; an Anti-Microbially Effective Medicinal Spice
American Journal of BioScience
Volume 3, Issue 1-1, February 2015, Pages: 1-6
Received: Jan. 5, 2015; Accepted: Jan. 8, 2015; Published: Jan. 14, 2015
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Kurhekar Jaya Vikas, Department of Microbiology, Bharati Vidyapeeth’s, Dr. Patangrao Kadam Mahavidyalaya, Sangli, Maharashtra, India
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Curcuma longa - haladi, a plant grown as a spice is an entity known to have significant antimicrobial potency. This study was carried out to analyze its bio-active components, with the help of High Performance Thin Layer Chromatography (HPTLC). Anti-microbial effects of C. longa were checked using aqueous and acetone extracts, against a few bacterial pathogens causing common infections. The aqueous extract was found effective in inhibiting the growth of eight pathogens (64% efficiency) while the acetone extract in inhibiting the growth of three pathogens (21% efficiency). Flavonoid analysis of aqueous extract by HPTLC showed peaks probably corresponding to Coumarins, Triterpenoids, Echinacosides, Eriodictyl, Cichoric acid and Caffeic acid derivatives, responsible for its anti-microbial activities. This natural bio resource may prove to be of significance in the armamentarium of antimicrobial agents.
Curcuma longa, Antimicrobial Activity, Bio-Active Components, MIC, HPTLC
To cite this article
Kurhekar Jaya Vikas, High Performance Thin Layer Chromatography Analysis of Bioactive Components from Curcuma longa; an Anti-Microbially Effective Medicinal Spice, American Journal of BioScience. Special Issue: Pharmacognosy with Reference to Evaluation of Medicinal Plants as an Alternative Therapy. Vol. 3, No. 1-1, 2015, pp. 1-6. doi: 10.11648/j.ajbio.s.2015030101.11
Azay-Milhau J., Ferrare K., Leroy J., Aubaterre J., Tournier M., Lajoix A., et al. (2013). Antihyperglycemic effect of a natural chicoric acid extract of chicory (Cichorium intybus L.): a comparative in vitro study with the effects of caffeic and ferulic acids. J. Ethnopharmacol. 150, 755–760
Bambode TS, Shukla VN, (1973). Antifungal Properties of Certain Plant Extracts Against Some Fungi, P.K.V. Res. J., 2, 1-8.
Baskar RG, Chezhiyan N, (2002). Strength and Wealth of Therapeutic Medicinal Plants in India – Role of Biotechnology in Medicinal and Aromatic Plants, Special Volume on Diseases, Ukaaz Publication, Hyderabad, VI, 149.
Bauer AW, Kirby WM, Sherris JC, Turek M, (1966). Antibiotic Sensitivity Testing by a Standardized Single Disc Method, Am. J. Clin. Pathol, 45, 493.
Bone K, (1991). Turmeric - The spice of life? Br. J. Phytotherapy, 2, 51-60.
Chandi CR, Dash SK, Mishra R., Azeemoddin G., (1999). ibid, 36(2), 133.
Charvat T. T., Lee D. J., Robinson W. E., Chamberlin A. R. (2006). Design, synthesis, and biological evaluation of chicoric acid analogs as inhibitors of HIV-1 integrase. Bioorg. Med. Chem. 14, 4552–4567.
Chawla AS, Handa SS, Sharma AK, Kalik BS, (1987). Plant anti-inflammatory agents, J. Sci. Industri. Res., 46, 214-223.
Ciddi V, Kaleab A, (2005). Anti-oxidants of plant origin, Indian J. Nat. Prod., 21 (4), 3.
Cunqin J, Haiming S, Wei J, Ke Z, Yong J, Mingbo Z, and Pengfei T, (12/2008). Metabolism of Echinacoside, a Good Antioxidant, in Rats: Isolation and Identification of Its Biliary Metabolites, Drug metabolism and disposition 37 (2), 431-8.
da Cunha FM, Duma D, Assreuy J, Buzzi FC, Niero R, Campos MM, Calixto JB (2004). Caffeic acid derivatives: in vitro and in vivo anti-inflammatory properties. Free Radic Res., 38 (11), 1241-53.
Dubey RC, Rana A, Shukla RK, (2005). Antibacterial activity of essential oils of some medicinal plants against certain certain human pathogens, Indian Drugs, 42(7): 443-446.
Finegold SM, Baron EJ, (1986). Diagnostic Microbiology, 7th Edn., The C.V. Mosby Company, St. Louis, 176.
Gangwar P, Kumar M, (2006). Antibacterial Properties and Phytochemical Analysis of the Essential Oil and Aqueous Extract of Mentha spicata (Spearmint), Ind. Drugs, 43: 55-58.
Harbourne JB, (1973). Phytochemical Methods, Toppan Company Ltd., Tokyo, Japan, 1-6.
Jain P. K. and Joshi H., (2012). Coumarin: Chemical and Pharmacological Profile, Journal of Applied Pharmaceutical Science, 02 (06); 236-240.
Joe B, Vijaykumar M, Lokesh BR, (2004). Biological properties of curcumin-cellular and molecular mechanisms of action. Crit. Rev. Food Sci. Nutr., 44: 97-111.
Kaushik P, Dhiman AK, (2000). Medicinal Plants and Raw Drugs of India, Singh B., Singh M.P., Dehradun, XII: 623.
Khan IA, Khanum A, (2002). Role of Biotechnology in Medicinal and Aromatic Plants, Special Volume on Diseases, Ukaaz Publications, Hyderabad, VI: 4-6.
Khandelwal KR, Practical Pharmacognosy, (2001). 8th edn., Nirali publication, 149-153.
King P. J., Robinson W. E., Jr. (1998). Resistance to the anti-human immunodeficiency virus type 1 compound L-chicoric acid results from a single mutation at amino acid 140 of integrase. J. Virol. 72, 8420–8424.
Kokate CK, Purohit AP, Gokhale SB, (2001). Pharmacognosy, 17th edition, Nirali Prakashan, Pune, 197, 445.
Limtrakul PS, Anuchapreeda, Buddhasukh D, (2004). Modulation of human multidrugresistance MDR-1 gene by natural curcuminoids. BMC Cancer, 4: 13.
Mahajan BK, (1989). Methods in Biostatistics, 4th edition, Jaypee brothers, New Delhi, 140-141.
Mateus FR, Gabriela T, Cristiani I, Banderó W, Jonatas ZK, Ana P de O, Jardel GI, Ricardo BZ, Luiz FFR, Margareth LA, Marcus VO, Juliano F, (2013). Biochemical Pharmacology, Volume 81, Issue 4, Eriodictyol: A flavonoid antagonist of the TRPV1 receptor with antioxidant activity, Front Chem. 1: 40.
Pluymers W., Neamati N., Pannecouque C., Fikkert V., Marchand C., Burke T. R., Jr., et al. (2000). Viral entry as the primary target for the anti-HIV activity of chicoric acid and its tetra-acetyl esters. Mol. Pharmacol. 58, 641–648.
Queffelec C., Bailly F., Mbemba G., Mouscadet J., Hayes S., Debyser Z., et al. (2008). Synthesis and antiviral properties of some polyphenols related to Salvia genus. Bioorg. Med. Chem. Lett. 18, 4736–4740.
Rawat S, Nijwante S, Jaiswal L, Laila F, (2005). Preparation and evaluation of a new facial scrub as a skin cosmetic, Indian J. Nat. Prod., 21(4): 31.
Salimath BP, Sundaresh CS, Srinivas L, (1986). Dietary components inhibit peroxidation in erythrocyte membrane Nutr. Res., 1171-1178.
Sastri BN, (1952). The wealth of India, Raw materials, CSIR, New Delhi, III: 168.
Sathe AV, (1998). Gharguti Aushadhe, 15th edn., Ganesh printers Pune, 336-337.
Sawant SY, (1974). Maharashtratil Divya Vanaushadhi, Continental publications, Pune, 273.
Scartezzini P, Speroni E, (2000). J.Ethnopharmacol., 71(1-2), 23.
Shekhawat PS, Prasada R, (1971). Antifungal Properties of some Plant Extracts, Ind. Phytopath, 24: 800-802.
Shrinivas, Prabhakaran KUS, (1987). Clinical bacteriological study of C.longa on conjunctivitis, Antiseptic, 84: 166-168.
Singh A, (1977). Practical Plant Physiology, Kalyani Publishers, New Delhi- Ludhiana, 94-116.
Soni KB, Rajan A, Kuttan R, (1992). Reversal of aflatoxin induced liver damage by turmeric and curcumin, Cancer Lett..
Kolesnikova SA, Lyakhova EG, Kalinovsky AI, Pushilin MA, Afiyatullov SS, Yurchenko EA, Dyshlovoy SA, Minh CV, and Stonik VA, (2013). Isolation, Structures, and Biological Activities of Triterpenoids from a Penares sp. Marine Sponge, J. Nat. Prod, J. Nat. Prod., 76 (9), 1746–1752.
Tang W, Eisenbrand G, (1992). Chinese Drugs of Plant Origin, Springer-Verlag: Berlin and Heidelberg, Germany, 401-415.
Tousch D., Lajoix A., Hosy E., Azay-Milhau J., Ferrare K., Jahannault C., et al. (2008). Chicoric acid, a new compound able to enhance insulin release and glucose uptake. Biochem. Biophys. Res. Comm. 377, 131–135.
Tsai Y., Chiu C., Chen J., Chan K., Lin S. (2012). Cytotoxic effects of Echinacea purpurea flower extracts and cichoric acid on human colon cancer cells through induction of apoptosis. J. Ethnopharm. 143, 914–919.
Vogel AI, (1966), Elementary Practical Organic Chemistry, Part 2, Qualitative Organic Analysis, 2nd Edn., CBS Publishers and Distributors, Delhi, 90-91, 163-164.
Xiao H., Wang J., Yuan L., Xiao C., Wang Y., Liu X. (2013). Chicoric acid induces apoptosis in 3T3-L1 preadipocytes through ROS-mediated PI3K/Akt and MAPK signaling pathways. J. Agric. Food Chem., 61, 1509–1520.
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