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Fish Behaviour and Immune Response as a Potential Indicator of Stress Caused by 4-Nonylphenol
American Journal of BioScience
Volume 3, Issue 6, November 2015, Pages: 278-283
Received: Nov. 17, 2015; Accepted: Nov. 27, 2015; Published: Dec. 30, 2015
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Madhu Sharma, Department of Zoology, Guru Nanak Dev University, Amritsar, Punjab, India
Pooja Chadha, Department of Zoology, Guru Nanak Dev University, Amritsar, Punjab, India
Manoj Kumar Borah, Department of Veterinary Pathology, Khalsa College of Veterinary and Animal Sciences, Amritsar, Punjab, India
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The present investigation was undertaken to study the stress response in fish Channa punctatus after treatment with 4-nonylphenol. Stress response was measured by studying the change in behaviour and immume system. After range finding experiment fish were exposed to 5 concentrations of 4-NP and LC50 was determined (LC50=1.27mg/l). Simultaneously behavioural response was observed for 96 hours of exposure. After LC50 determination three sublethal concentrations were decided and fish were exposed for 24, 48, 72 and 96 hours and differential leucocytes count was calculated. Significant change in the number of lymphocytes, monocytes and neutrophils was seen. This study concludes that fish immune system and behaviour are most sensitive parameters to study the stress level in fish.
Behaviour, Leucocytes, Lymphocyte, Monocytes, Neutrophils
To cite this article
Madhu Sharma, Pooja Chadha, Manoj Kumar Borah, Fish Behaviour and Immune Response as a Potential Indicator of Stress Caused by 4-Nonylphenol, American Journal of BioScience. Vol. 3, No. 6, 2015, pp. 278-283. doi: 10.11648/j.ajbio.20150306.21
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This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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