Low temperature or cold stress is one of the major abiotic stresses for bean seeds production. The objective of this study was to analyze the effect of cold stress on Phaseolusvulgaris l seeds by doing different types of tests(germination studies, respiration response, conductivity test, amylase and catalase test) at different temperatures like room temperature, at 3° C and at - 19° C. Seeds were germinated in germination box. Germination percentage, rate, seed vigour and germination index was measured. Respiration response was also analyzed. The procedure does not quantify a respiration rate, but it indicates that respiration rate can be compared across different treatments. Observation from conductivity measurement shows that leachate solution of -19° C gives low conductivity whereas 3° C gives moderate and at normal room temperature it was high. Low temperature showed high amylase as well as catalase activity. Room temperature showed a good response against few parameters but not in enzyme activity.
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