Study of the Extract Activities of Buas buas Leaves (Premna pubescens) as Immunostimulant on Rats (Rattus novegicus)
American Journal of BioScience
Volume 2, Issue 6, November 2014, Pages: 244-250
Received: Nov. 28, 2014; Accepted: Dec. 14, 2014; Published: Dec. 19, 2014
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Authors
Martina Restuati, Biology Education Department, Faculty of Mathematic and Science, State University of Medan, Medan, Indonesia
Syafruddin Ilyas, Biology Department, Faculty of Mathematic and Science, University of North Sumatra, Medan, Indonesia
Salomo Hutahaean, Biology Department, Faculty of Mathematic and Science, University of North Sumatra, Medan, Indonesia
Herbert Sipahutar, Biology Education Department, Faculty of Mathematic and Science, State University of Medan, Medan, Indonesia
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Abstract
Buas buas (Premna pubescens) is one of the plants in Indonesia which is believed to have medicinal properties, but it is not certain. This plant is consumed by the Malay community, one of the tribes in North Sumatra - Indonesia, as vegetables, especially during the fasting month. This paper describes the effects of ethanol extract of leaves of Buas buas as an immunostimulant in rats (Rattus norvegicus). The experiments were performed on 24 tail of male rats, which were three months old, weighing 140-180 g. There were four groups of experiment ie Group A0 was given Carboxy Methyl Cellulose mice (CMC) (control group), group A1 was given 500 mg/ kg bw of Ethanol Extracts of Buas buas. Group A2, given ethanol extracts of Buas buas 500 mg / kg bw + SRBC, and the group A3 only given Sheep Red Blood Cell (SRBC). After this experiment, apigenin levels were analyzed by High Performance Liquid Chromatography (HPLC) Agilent 1220. Then the activity of immunostimulation is determinated by measuring the leukocyte count, percentage of lymphocytes, antibody titers, the levels of immunoglobulin M (IgM), immunoglobulin G (IgG), and lysozyme with ABX Micros 60. The group A2 is seen the highest group of in the number of leukocytes, antibody titer levels, imonoglobulin G (IgG), and imonoglobulin M. The amount of lysozyme owned group A2 is 0:04 ug / ml, whereas other treatments to obtain results that are not different relative significantly. Provision of Ethanol Extracts of Buas buas on mice can increase the leukocytes, lymphocytes, antibody titers, IgM, IgG and Lysozyme.
Keywords
Premna Pubescens, Titer Antibodies, Lymphocytes, SRBC, IgG, IgM, Lysozyme
To cite this article
Martina Restuati, Syafruddin Ilyas, Salomo Hutahaean, Herbert Sipahutar, Study of the Extract Activities of Buas buas Leaves (Premna pubescens) as Immunostimulant on Rats (Rattus novegicus), American Journal of BioScience. Vol. 2, No. 6, 2014, pp. 244-250. doi: 10.11648/j.ajbio.20140206.19
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