Study of the Extract Activities of Buas buas Leaves (Premna pubescens) as Immunostimulant on Rats (Rattus novegicus)
American Journal of BioScience
Volume 2, Issue 6, November 2014, Pages: 244-250
Received: Nov. 28, 2014;
Accepted: Dec. 14, 2014;
Published: Dec. 19, 2014
Views 2887 Downloads 157
Martina Restuati, Biology Education Department, Faculty of Mathematic and Science, State University of Medan, Medan, Indonesia
Syafruddin Ilyas, Biology Department, Faculty of Mathematic and Science, University of North Sumatra, Medan, Indonesia
Salomo Hutahaean, Biology Department, Faculty of Mathematic and Science, University of North Sumatra, Medan, Indonesia
Herbert Sipahutar, Biology Education Department, Faculty of Mathematic and Science, State University of Medan, Medan, Indonesia
Buas buas (Premna pubescens) is one of the plants in Indonesia which is believed to have medicinal properties, but it is not certain. This plant is consumed by the Malay community, one of the tribes in North Sumatra - Indonesia, as vegetables, especially during the fasting month. This paper describes the effects of ethanol extract of leaves of Buas buas as an immunostimulant in rats (Rattus norvegicus). The experiments were performed on 24 tail of male rats, which were three months old, weighing 140-180 g. There were four groups of experiment ie Group A0 was given Carboxy Methyl Cellulose mice (CMC) (control group), group A1 was given 500 mg/ kg bw of Ethanol Extracts of Buas buas. Group A2, given ethanol extracts of Buas buas 500 mg / kg bw + SRBC, and the group A3 only given Sheep Red Blood Cell (SRBC). After this experiment, apigenin levels were analyzed by High Performance Liquid Chromatography (HPLC) Agilent 1220. Then the activity of immunostimulation is determinated by measuring the leukocyte count, percentage of lymphocytes, antibody titers, the levels of immunoglobulin M (IgM), immunoglobulin G (IgG), and lysozyme with ABX Micros 60. The group A2 is seen the highest group of in the number of leukocytes, antibody titer levels, imonoglobulin G (IgG), and imonoglobulin M. The amount of lysozyme owned group A2 is 0:04 ug / ml, whereas other treatments to obtain results that are not different relative significantly. Provision of Ethanol Extracts of Buas buas on mice can increase the leukocytes, lymphocytes, antibody titers, IgM, IgG and Lysozyme.
Study of the Extract Activities of Buas buas Leaves (Premna pubescens) as Immunostimulant on Rats (Rattus novegicus), American Journal of BioScience.
Vol. 2, No. 6,
2014, pp. 244-250.
Lie, T., et. all., 2010. Flavonoid, Phenol and Polysaccharide Contents of Echinacea purpurea L. and Its Immunostimulant Capacity In Vitro. International Journ. of Environmental Science and Development. Vol. 1 No. 1. http://dx.doi.org/10.7763/ijesd.2010.v1.2
Thiruvenkatasubramaniam, R., Jayakar, B. 2010. Anti-hypreglycemic and anti-hyperlipidemic activities of Premna corymbosa (Burm.F) Rottl on Streptozotocin induced diabetic rats. Der Pharmacia Lettre. 2 (1) 505-509.
Gates M.A, Vitonis, A.F, Tworoger S.S, Rosner, B, Ernstoff, L.T, Hankinson, S.E, dan Cramer D.W, 2009. Flavonoid intake and ovarium cancer risk in a Polution based case-control study. International Journal of Cancer. 124, 1918-1925. http://dx.doi.org/10.1002/ijc.24151.
Chen, D., Piwowar, K.R.L., Chen, M.S., Dou, Q.P, 2007. Inhibition of Proteasome Activity by the Dietary Flavonoid Apigenin is Associated with Growth Inhibition in Cultured Breast Cancer Cells and Xe7nografts. Breast Cancer Research. 9. 1-8. http://dx.doi.org/10.1186/bcr1797.
Fang, J., Xia, C., Cao. Z., Zheng, J.Z., Reed, E., dan Jiang, B.H. 2005. Apigenin inhibits VEGF and HIF-1 expression via P13K/AKT/ p7056K1 and HDM2/p53 pathways. The FASEB journal.19. 342-353. http://dx.doi.org/10.1096/fj.04-2175com
Zheng. P.W, Chiang. L.C, Lin, C.C. 2005. Apigenin Induced Apoptosis through p53-dependent pathway in human Cervikal Carcinova Cells. Life Science. Volume 76. Issue 12. http://dx.doi.org/10.1016/j.lfs.2004.08.023
Torkin R. Lavoie JF. Kaplan DR. Yeger H (2005). Induction of Caspase-Dependent, P-53 Mediated Apoptosis by Apigenin in Human Neuro blastoma. Molecolar Cancer Theurapetic. Vol 4. Edition 1. American Association for Cancer research.
Bairwa, M.K., Jakhar, J.K., Y. Satyanarayana., Reddy, A.D, 2012. Animal and Plant Originated Immunostimulants Used in Aquaculture. Journal of Natural Product and Plant Resource. 2 (3): 397-400.
Ogier de Baulny, M., Quentell, C., Vournier, V. Lamour, F and R. Guevello. 1996. Effect of long-term oral administration of β-glucan as an immunostimulant or an adjuvant on some non-specific parameters of the immune response of turbot Scophthalmus maximus. Diseases of aquatic organisms. Vol 26: 139 – 147. http://dx.doi.org/10.3354/dao026139
Khumar, S., Gupta, P., Sharma S. and D Khumar. 2011. A Review on Immunostimulatory plant. Journal of Chinese Integrative Medicine. Vol. 9 No.2: 117-128. http://dx.doi.org/10.3736/jcim20110201.
Jian, J dan Wu, Z. 2004. Influence of Traditional Chinese Medicine on Non-Specific Immunity of Jian Carp (Cyprinus carpio var. Jian). Fish and Shellfish Immunology. 16. 185-191. http://dx.doi.org/10.1016/s1050-4648(03)00062-7.
Ellis, A. 1988. Fish faccination. Academic Press, San Diego, C.A. USA. 1-16
Charles River Laboratories, 1998. Baseline Hematology and Clinical Chemistry Value for Charles River Wistar Rats-(CRL: (WI) BR) as a Function of Sex and Age. Technical Bulletin.
Siahaan, P.M. 2010. Immunologi. FMIPA UNIMED. Medan.
Farhath, S., Vijaya dan Vimal. 2013. Immunomodulatory activity of geranial acetate, gingerol, and eudenol essential oils: evidence for humoral and cell-mediated responses. Avicenna Journal of Phytomedicine. 1-7.
Roitt, I. 2001. Roitt’s Essential Immunology, 8th ed.Blackwell Science.
Kannan, M., Singh, A.R., Kumar, T.A., Jegatheswari, P., dan Subburayalu, S. 2007. Studies on Immuno-bioactivities of Nyctanthes arbortritis (Oleacea). African Journal of Microbiology Research, 1 (6). 088-091.
Shiney, R.B, Ganesh P, and R. S. Khumar. 2012. Phytochemical Screening of Coleus aromaticus and Leucas aspera and Their Antibacterial Activity against Entheric Phatogens. International Journal of Pharmaceutical and Biological Archives. 3(1): 162-166.