Protective Effect of Pseudomonas Fluorescens as a Probiotic in Controlling Fish Pathogens
American Journal of BioScience
Volume 2, Issue 5, September 2014, Pages: 175-181
Received: Jul. 31, 2014; Accepted: Aug. 27, 2014; Published: Sep. 10, 2014
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Authors
Nour Eissa, Aquatic Diseases Laboratory, Aquaculture Division, National Institute of Oceanography and Fisheries, 101 Kaser El-Aini Street, Cairo 11516, Egypt
ElSayed Abou El-Gheit, Aquatic Diseases Laboratory, Aquaculture Division, National Institute of Oceanography and Fisheries, 101 Kaser El-Aini Street, Cairo 11516, Egypt
Adel A. Shaheen, Department of Fish Diseases and Management, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh, 13736, Egypt
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Abstract
The use of Pseudomonas fluorscens isolates as biological control agents against two fish pathogens; Pseudomonas angulliseptica and Streptococcus faecium in Nile tilapia were investigated in vivo and vitro. Pseudomonas fluorscens biovars I, II & III were tested in vitro using the agar diffusion method and showed effectiveness in inhibiting growth of P. angulliseptica and Strep. faecium. Pseudomonas fluorscens biovar II was the most effective with largest inhibition zones against both pathogens. For oral administration, P. fluorscens biovars were incorporated into the mixed feed diet. A significant reduction in mortality rate and a significant increase in hematological parameters, total protein, and globulin in fish groups fed Pseudomonas fluorscens incorporated diet following challenge by P. angulliseptica and Strep. faecium. It could be concluded that P. fluorescens has a protective effect against different Nile Tilapia pathogens and could be used as a probiotic bacteria and an eco-friendly alternative measure to chemical antimicrobials and further research studies to clarify its protective mechanisms on cellular and molecular levels.
Keywords
Probiotics, Pseudomonas, Fish Diseases, Biological Control, Hematological Parameters
To cite this article
Nour Eissa, ElSayed Abou El-Gheit, Adel A. Shaheen, Protective Effect of Pseudomonas Fluorescens as a Probiotic in Controlling Fish Pathogens, American Journal of BioScience. Vol. 2, No. 5, 2014, pp. 175-181. doi: 10.11648/j.ajbio.20140205.12
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