Molecular Characterization and Phylogenetic Analysis of Growth Hormone cDNA Sequence from the Acipenser Persicus
American Journal of BioScience
Volume 2, Issue 2, March 2014, Pages: 79-83
Received: Mar. 6, 2014; Accepted: Apr. 11, 2014; Published: Apr. 20, 2014
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Authors
E. Nasr, Islamic Azad University, Meshgin Shahr Iran
H. G. Hovhannisyan, Scientific and Production Center “Armbiotechnology”, National Academy of Sciences of Armenia, Yerevan, Armenia
M. Pourkazemi, International sturgeon Research institute, Rasht, Iran
L. Azizzadeh, International sturgeon Research institute, Rasht, Iran
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Abstract
The aim of this study was to investigate the amount of variation between Acipenseridea family and also, study amount of phylogenetic variation between Persian sturgeon (Acipenser persicus) with other Acipenseridea regarding to the Growth Hormon (GH) gene. In this study, pre growth hormone gene of Persian sturgeon was identified for first time and applied to gene bank (JN604534.1, 2011). The total RNA was extracted from pituitary gland of Persian Sturgeon, cDNA was synthesized. The full-length cDNA sequence of Persian sturgeon contains a 645 nucleotide open reading frame, which encodes a peptide of 214 amino acids. The position of the signal peptide cleavage site was predicted to be at position 72. After cleaving of a signal peptide of 24 amino acid resi¬dues and a mature peptide of 190 aa formed. Multiple sequence alignments and Phylogenic tree were performed by using the MEGA5 program. By sequence alignment and phylogenetic analysis of amino acid residues indicated that GH widely conserved in other species were identified. The GH nucleic acid and amino acid residue sequences of Persian sturgeon had a highest similarity to these of other Acipenseridea as well as mammalian, followed by those of Anguiliformes
Keywords
Persian Sturgeon, Growth Hormone, cDNA, Phylogenetic Analysis
To cite this article
E. Nasr, H. G. Hovhannisyan, M. Pourkazemi, L. Azizzadeh, Molecular Characterization and Phylogenetic Analysis of Growth Hormone cDNA Sequence from the Acipenser Persicus, American Journal of BioScience. Vol. 2, No. 2, 2014, pp. 79-83. doi: 10.11648/j.ajbio.20140202.20
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