Lipid-Modulating Effects of Aqueous Extract of Rubus Occidentalis in Hepatocarcinoma HepG2 Cells
American Journal of BioScience
Volume 2, Issue 2, March 2014, Pages: 64-69
Received: Jan. 22, 2014; Accepted: Apr. 8, 2014; Published: Apr. 10, 2014
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Young-Sook Moon, Department of Pharmacology, Chosun University Medical School, Gwangju 501-759, Republic of Korea
Ju-Hee Han, Department of Pharmacology, Chosun University Medical School, Gwangju 501-759, Republic of Korea
Tae-Bum Lee, Gochang Black Raspberry Research Institute, Gochang-gun, Jeollabuk-do 585-943, Republic of Korea
Ji-Wung Kwon, Gochang Black Raspberry Research Institute, Gochang-gun, Jeollabuk-do 585-943, Republic of Korea
Cheol-Hee Choi, Department of Pharmacology, Chosun University Medical School, Gwangju 501-759, Republic of Korea
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Little knowledge exists on the lipid-modulating effect of Rubus occidentalis (RO). The present study investigated the molecular mechanisms of lipid-modulating effects of aqueous extract of RO (ROW) in hepatocarcinoma HepG2 cells. ROW decreased apolipoprotein B100 (ApoB100)/apolipoprotein A-1 (ApoA-1) ratio. ROW increased the expression of LDL-receptor (LDL-R). ROW decreased the gene expression of sterol regulatory element-binding protein 1c (SREBP-1c) as well as fatty acid synthase in a concentration-dependent manner. ROW not only down-regulated gene expression of SREBP-2 and HMG-CoA synthase mRNA expression, but also inhibited HMG-CoA reductase activity in a concentration-dependent manner (IC50 = 240.6 μg/ml). These results indicate that ROW decreases the level of bad cholesterol and increases good cholesterol levels by lowering the gene expression of transcription factors, SREBP-1c and SREBP-2, and by inhibiting the expression and/or activity of their downstream enzymes such as fatty acid synthase and HMG-CoA synthase and reductase.
Rubus Occidentalis, Apolipoprotein B100, Apolipoprotein A-1, LDL-Receptor, Sterol Regulatory Element-Binding Protein (SREBP), HMG-CoA Reductase
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Young-Sook Moon, Ju-Hee Han, Tae-Bum Lee, Ji-Wung Kwon, Cheol-Hee Choi, Lipid-Modulating Effects of Aqueous Extract of Rubus Occidentalis in Hepatocarcinoma HepG2 Cells, American Journal of BioScience. Vol. 2, No. 2, 2014, pp. 64-69. doi: 10.11648/j.ajbio.20140202.18
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