Genotyping of Erythrocyte Binding Antigen 175 KD (eba-175) Alleles of Plasmodium Falciparum-Malaria Using Nested-PCR in South-East of Iran
American Journal of BioScience
Volume 2, Issue 2, March 2014, Pages: 56-59
Received: Mar. 12, 2014;
Accepted: Apr. 8, 2014;
Published: Apr. 10, 2014
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Ebrahimzadeh Adel, Department of Medical Parasitology and Mycology and Membership of Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, Iran
Jamshidi Ali, Department of Medical Parasitology and Mycology, Zahedan University of Medical Sciences, Zahedan, Iran
The Erythrocyte Binding Antigen-175 (EBA-175) on Plasmodium falciparum (P.falciparum) merozoites mediates sialic acid dependent binding to glycophorin a on host erythrocytes and, therefore, plays a crucial role in cell invasion. Dimorphic allele segments have been found in its encoding gene with in FCR-3 strains (F-segment) and CAMP strains (C-segment). This study was designed to determine the distribution of EBA-175 alleles of P.falciparum in the South-East of Iran. Nested Polymerase Chain Reaction method used with specific primers, which improves the two fragments of the EBA-175 gene. Ninety-four microscopically positive blood samples were collected from the infected P.falciparum malaria patients from four different districts. The 88 out of 94 confirmed P.falciparum samples were successfully scored for EBA-175. The allelic genotyping exhibited CAMP strains (714 bp) and FCR-3 strains (795 bp) in 31(32.97%) and 49(52.12%) cases, respectively and 8 cases (8.51%) showed mixed allelic CAMP/FCR-3 infections. The two fragments of dimorphic EBA-175 gene were observed and the FCR-3 allele was more prevalent in South-East of Iran. This distributional pattern should be considered in designing to control P. falciparum malaria in the region.
Genotyping of Erythrocyte Binding Antigen 175 KD (eba-175) Alleles of Plasmodium Falciparum-Malaria Using Nested-PCR in South-East of Iran, American Journal of BioScience.
Vol. 2, No. 2,
2014, pp. 56-59.
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