Stimulated Biodegradation of Spent Lubricating Motor Oil in Soil Amended with Animal Droppings
American Journal of BioScience
Volume 2, Issue 1, January 2014, Pages: 19-27
Received: Dec. 8, 2013; Published: Jan. 10, 2014
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Authors
Stanley Chukwudozie Onuoha, Department of Biotechnology, Ebonyi State University, PMB 053 Abakaliki, Ebonyi State, Nigeria
Edna Ifeoma Chukwura, Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria
Kayode Fatokun, Department of Agriculture, University of Zululand, PMB X1001 KwaDlangezwa 3886, South Africa
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Abstract
The potentials of organic wastes from animal droppings as bioremediation alternative for soils spiked with waste-lubricating motor oil (spent oil) was evaluated. The rate of biodegradation of the spent oil was studied for a period of 6 months under laboratory condition. The result of the microbial counts for soils spiked with 5000 mgkg-1(0.5%) spent oil has its total heterotrophic bacterial count in unamended control soil ranging from 2.71±0.09 to 7.21±0.25 x 106 CFU/g of soil, while those of soil amended with cow droppings (CD) ranged from 16.0±1.01 to 47.90±0.36x 106 CFU/g of soil and those of soil amended with goat droppings (GD) and poultry manure(PM) ranged from 16.6±0.6 to 57.9±0.15 x 106 and 18.00±0.20×106 to 60.80± 1.19 ×106 CFU/g of soil respectively. The counts of hydrocarbon-utilizing bacteria (HUB) in unamended control soil ranged from 0.93±0.11 to 2.10±0.15 ×106 CFU/g of soil. The count of HUB in PM amended soil was significantly higher than those amended with goat droppings (GD), while those of goat dropping were higher than those of cow dung. The HUB count in GD amended soil ranged from 8.07± 0.12x106 to 67.70±0.68 x 106 cfu/g of soil. The THB and HUB counts for soils spiked with 25000 mg/kg (2.5%) spent oil exhibited a similar trend as was observed for soil spiked with 0.5% spent oil. Evaluation by the first-order kinetic model which utilized combined data for the entire period revealed that PM with biodegradation rate constant of 0.2332 day-1 and half-life of 2.97days was better in stimulating biodegradation of oil at higher concentration, while GD with a biodegradation constant of 0.3253day-1 and half-life of 2.13 days performed better at low pollution when compared to that of PM and CD.
Keywords
Biodegradation, Waste-Lubricating Oil, Bacteria, Organic Waste, Hydrocarbon
To cite this article
Stanley Chukwudozie Onuoha, Edna Ifeoma Chukwura, Kayode Fatokun, Stimulated Biodegradation of Spent Lubricating Motor Oil in Soil Amended with Animal Droppings, American Journal of BioScience. Vol. 2, No. 1, 2014, pp. 19-27. doi: 10.11648/j.ajbio.20140201.14
References
[1]
Abioye, O.P., Abdul Aziz, A and Agamuthu, P.(2009b). Stimulated biodegradation of used lubricating oil in soil using organic wastes. Malaysian Journal of Science. 28 (2):127-133.
[2]
Abioye, O.P., Abdul Aziz, A and Agamuthu, P (2010). Enhanced biodegradation of used engine Oil in soil amended with organic wastes. Water Air and Soil pollution.209: 173- 179.
[3]
Adesodun, J.K. and Mbagwu, J.S.C (2008). Biodegradation of waste lubricating petroleum oil in a tropical alfisol as mediated by animal droppings. Bioresource Technology, 99: 5659 – 5665.
[4]
American Health Public Health Public Association (APHA) (1998). Standard Method for the examination of water and waste water, Washigton, DC
[5]
Antai, S.P and Mgbomo (1989). Distribution of hydrocarbon utilizing bacteria in oil-spill areas. Microbiology Letters, 40:137-143
[6]
Bento, F.M., Carmago, F.O.A., Okeke, B.C., Frankenberger, W.T (2005). Comparative bioremediation of soil contaminated with diesel oil by natural attenuation, biostimulation and bioaugmentation. Bioresource Technology, 96:1049-1055.
[7]
Bossert, I and Bartha, R. (1984). The fate of petroleum in soil ecosystems. In: Atlas, R.M. (Ed.), Petroleum Microbiology, Macmillan, New York, pp. 435–473.
[8]
Buchanan R. V and Gibbons N. E. (1994). Bergey’s Manual of Determinative Bacteriology, Williams and Wilkins Co., Baltimore, USA.
[9]
Cho, B.H., Chino, H., Tsuji, H., Kunito, T., Nagaoka, K., Otsuka, S., Yamashita, K., Matsumoto, S and Oyaiz, H (1997). Laboratory –scale bioremediation of a contaminated soil of Kuwait with amendment materials. Chemosphere, 35 (7), 1599-1611.
[10]
Clemente, A.R., anazawa, T.A and Durrant, L.R (2001). Biodegradation of polycyclic aromatic hydrocarbons by soil fungi. Brazilian Journal of Microbiology, 32:255-261.
[11]
Dibble, T.T and Bartha, R (1979). Effect of environmental parameters on the biodegradation of oil sludge. Applied Environmental Microbiology, 37:729-739.
[12]
Eggen, T. (1999). Application of fungal substrate from commercial mushroom production Pleurotus ostreatus for bioremediation of creosote contaminated soil. International Biodeterioration and Biodegradation, 44:117-126.
[13]
Foght, J.M. and Westlake, D.W.S. (1987) Biodegradation of hydrocarbons in freshwater. In: Vandermeulen and Hrudey (Ed), Oil in Freshwater: Chemistry, Biology, Countermeasure Technology. Pergamon Press, New York, pp217-230.
[14]
Ijah, U.J.J (1998). Studies on relative capabilities of bacterial and Yeasts isolates from tropical soils in degrading crude oil. Waste Management, 18: 293-299.
[15]
Ijah, U.J.J and Antai, S.P (2003a). The potential use of chicken-drop microorganisms for oil spill remediation. The Environmentalist, 23:89-95
[16]
Ijah, U.J.J and Antai, S.P. (2003b) Removal of Nigerian light crude oil in soil over a 12–month period. International Biodeterioration Biodegradation, 51: 93-99.
[17]
Ijah, U.J.J and Ndana, M (2000). Stimulated biodegradation of crude oil in soil amended with periwinkle shells. The Environmentalist, 23: 249-254.
[18]
Joo, H.S., Shoda, M and Phae. C.G (2007). Degradation of diesel oil in soil using a food waste composting process. Biodegradation, 18:597-605
[19]
Joo, H.S., Phae. C.G and Ryu, J.Y (2001). Comparison and analysis of characteristics for recycling of multifarious food waste. J KOWREC, 9: 117-124.
[20]
Kaplan, C.W and Kitt, C.L (2004). Bacterial succession in a petroleum land treatment unit. Applied Environmental Microbiology, 70:1777-1786.
[21]
Kyung-Hwa, B.Y., Byung-Dae O., Hee-Mock., Hee-Sik, K and Sook, L (2006). Biodegradation of aliphatic and aromatic hydrocarbon by Norcadia sp. H17-1. Geomicrobiology Journal, 23 (5): 253 – 259.
[22]
Ladousse, A. and Tramier, B. (1991) Results of 12 years of research in spilled oil bioremediation: Inipol EAP 22, Proceedings of 1991 Oil Spill Conference. American Petroleum Institute, Washington, DC, pp577-581.
[23]
Lau, K.L., Tsang, Y.Y., Chiu, S.W. (2003). Use of spent mushroom compost to bioremediate PAH-contaminated samples. Chemosphere, 52 (9):1539-1546.
[24]
Leahy, J.G., Colwell, R.R. (1990) Microbial degradation of hydrocarbons in the environment. Microbiological Review, 54: 305-315.
[25]
Lloyd, C.A and Cackette, T.A (2001). Diesel engines: Environmental impact & control. Air and waste management Association, 51: 805-847.
[26]
Macnaughton, S.J., Stephen, J.R., Venosa, A.O., Davis, G.A., Chang, Y.J and White, D.C. (1999) Microbial population changes during bioremediation of an experimental oil spill. Applied Environmental Microbiology, 65: 3566-3574.
[27]
Majid, Z.; V. Mnouchehr and K.A. Sussan (2008). Naphthalene metabolism in Norcardia Otitidis caviarum stream. TSHI, a moderately thermophilic microorganism. Chemosphere, 72: 905-909
[28]
Mills, A.L., Brueil, C and Colwell, R.R (1978). Enumeration of petroleum degrading marine microorganisms by the most probable number method. Canadian Journal of Microbiology, 22: 552-557.
[29]
Namkoong, W., Hwang, E., Park, J and Choi, J. (2002). Bioremediation of diesel- contaminated soil with composting. Environmental Pollution, 119: 23-31.
[30]
Odokuma, L. O. and Ibor, M. N. (2002) Nitrogen fixing bacteria enhanced bioremediation of crude oil polluted soil. Global Journal of Pure and Applied Sciences. 8 (4): 455-468.
[31]
Odokuma, L.O and Dickson, A.A. (2003) Bioremediation of a crude oil – polluted tropical rain forest soil. Global Journal of Environmental Sciences, 2: 29-40
[32]
Odokuma, L.O and Okpokwasili, G.C(1993). Seasonal ecology of hydrocarbon-utilizing microbes in the surface water of a river Environ.Mon. Assess 27(3) 175-191.
[33]
Okolo, J.C., Amadi, E.N and Odu, C.T.I (2005). Effects of soil treatments containing poutry manure on crude oil degradation in a sandy loam soil. Applied Ecology and environmental Research, 3 (1): 47-53.
[34]
Onuoha, S.C., Olugbue, V.U., Uraku, J.A and Uchendu, D.O (2011). Biodegradation potentials of hydrocarbon degraders from waste –lubricting oil spilled soils in Ebonyi State, Nigeria. International Journal of Agriculture and Biology, 13:586-590.
[35]
Philips, J.C., Atlas, R.M. (2005). Bioremediation of contaminated soil and aquifers. In: Bioremediation: Applied Microbial solution for real-world environmental clean Up, Atlas, R.M., and Jim, C.P(ed) ASM press, ISBN 1-55591-239-2, Washington, D.C, pp.139
[36]
Quatrini, P., Scaglione, G., De Pasquale, C., Reila, S and Puglia, A.M. (2008) Isolation of Gram-positive n-alkane degraders from a hydrocarbon contaminated Mediterranean shoreline. Journal of Applied Microbiology, 104: 251- 259.
[37]
Schaefer, M and Juliane, F (2007). The influence of earthworm and organic additives on the biodegradation of oil contaminated soil. Applied Ecology, 36: 53-62.
[38]
Ting, Y.P., Hu, H.L and Tan, H.M (1999). Bioremediation of petroleum hydrocarbon in soil microcosm. Resource and Environmental Biotechnology, 2: 197-218.
[39]
Trejo-Hernandez, M..R., Lopez-Munguia, A.R and Ramirez, Q. (2001). Residual compost of Agaricus biosporus as a source of crude Laccase for enzymatic oxidation of phenolic compounds. Process Biochemistry, 36: 635-639.
[40]
Van Hamme, J.D., Singh, A and Ward, O.P. (2003). Recent advances in petroleum Microbiology. Molecular Biology Review, 67: 503-549.
[41]
Vasudevan,N and Rajaram, P (2001). Bioremediation of oil sludge contaminated soil. Environment International. 26: 409-411.
[42]
Williams, C.M., Grimes, J.L and Mikelssen, R.L (1999). The use of poultry litter as co-substrate and source of inorganic nutrient and microorganisms for the ex-situ biodegradation of petroleum compounds. Poultry litter, 78, 956-964.
[43]
Yeung, P.Y., Johnson, R.L and Xu, J.G. (1997) Biodegradation of petroleum hydrocarbons in soil as affected by heating and forced aeration. Journal Environmental Quality, 26: 1511- 1516.
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