Diversity, Bioactivity and Drug Development of Cultivable Actinobacteria in Six Species of Bird Feces
American Journal of BioScience
Volume 2, Issue 1, January 2014, Pages: 13-18
Received: Dec. 12, 2013;
Published: Dec. 30, 2013
Views 3015 Downloads 163
Xiu Chen, Yunnan Institute of Microbiology, Yunnan University, 650091 Kunming, Yunnan, P. R. China
Shumei Qiu, Yunnan Wild Animal Park, 650218 Kunming, Yunnan, China
Yi Jiang, Yunnan Institute of Microbiology, Yunnan University, 650091 Kunming, Yunnan, P. R. China
Li Han, Institute of Microbial pharmaceuticals, College of Life and Health Science, Northeastern University, 110819 Shenyang, P. R. China
Xueshi Huang, Institute of Microbial pharmaceuticals, College of Life and Health Science, Northeastern University, 110819 Shenyang, P. R. China
Chenglin Jiang, Yunnan Institute of Microbiology, Yunnan University, 650091 Kunming, Yunnan, P. R. China
In order to provide new lead compounds of drugs and other useful products, the diversity and some bioactivities of cultivable actinobacteria in bird feces were studied．Six species of bird fecal samples were collected from Yunnan Wild Animal Park and bank of Dian Lake. The pure cultures of actinobacteria were isolated from these samples by using 6 different media. The partial 16S rRNA gene sequences of 207 selected strains were determined, the phylogenetic analysis was carried out, and antimicrobial activities were determined by using agar diffusion method. The key biosynthase genes (CYP, AHBA and SAL) of antibiotics were also detected. Total 28 genera of actinobacteria from all the samples were identified. 18 genera were identified from Pavo cristatus feces, and the actinomycete community was the most complex. These actinobacteria had wide inhibition against 10 test microbes. In 30, 25, and 36 strains of actinobacteria isolated from Pavo cristatus, Grus japonensis and Larus ridibundus feces respectively, average 22.2%, 8.9% and 40.8% strains had the key biosynthase genes encoding CYP, AHBA and SAL. More than 30 bioactive secondary metabolites from several actinomycete strains of bird feces were isolated and characterized. The study shows that birds fecal actinobacteria are a new potential source for discovering new drugs and other industry products.
Diversity, Bioactivity and Drug Development of Cultivable Actinobacteria in Six Species of Bird Feces, American Journal of BioScience.
Vol. 2, No. 1,
2014, pp. 13-18.
Berdy J, Thoughts and facts about antibiotics: Where we are now and where we are heading. J Antibiot, 2012; 65(8): 385–395.
Cao YR, Jiang Y, Li YL, Chen X, Jin RX, and He WX, Isolation methods and diversity of culturable fecal actinobacteria associated with Panthera tigris tigris in Yunnan Safari Park. Acta Microbiologica Sinica, 2012; 52(7): 816－824.
Jiang Y, Cao YR, Han L, Jin RX, Zheng D, He WX, Li YL, and Huang XS, Diversity and bioactivity of culturable actinobacteria from 5 species of animal feces. Acta Microbiologica Sinica, 2012; 52(10): 1282－1289.
Hayakawa M, and Nonomura H, Humic acid-vitamin agar, a new medium for the selective isolation of soil actinobacterias. J Ferment Technol, 1987; 65: 501-509.
Cui XL, Mao PH, Zeng M, Xu LH, and Jiang CL, Streptomonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae. Int. J. Syst. Evol. Microbiol, 2001; 51: 357- 363.
Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, and Chun J, Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol, 2012; 62: 716-721.
Kim BG, Lee MJ, Seo J, Hwang YB, Lee MY, Han K, Sherman DH, and Kim ES Identification of functionally clustered nystatin-like biosynthetic genes in a rare actinobacterias, Pseudonocardia autotrophica. J Ind Microbiol Biotechnol, 2009; 36(11):1425-1434.
Kim CG, Yu TW, Fryhle CB, Handa S, and Floss HG, 3-Amino-5-hydroxybenzoic acid synthase, the terminal enzyme in the formation of the precursor of mC7N units in rifamycin and related antibiotics. J Biol Chem 273, 1998; (11): 6030-6040.
Knirschová R, Nováková R, Fecková L, Timko J, Turna J, Bistáková J, and Kormanec J, Multiple regulatory genes in the salinomycin biosynthetic gene cluster of Streptomyces albus CCM 4719. Folia Microbiol (Praha), 2007; 52(4): 359-65.
Stackebrandt E, and Goebel BM, Taxonomic note: a place for DNA-DNA reassociation and 16s rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol, 1994; 44(4): 846-849.
Xu LH, Li WJ, Liu ZH, and Jiang CL, Actinobacteria taxonomy. Acedemic Press, Beijing, 2007.
Lechevalier MP, and Lechevalier HA, Chemical composition as a criterion in the classification of aerobic actinobacterias. Int J Syst Bacteriol, 1970: 20: 435-443.
Lechevalier MP, and Lechevalier HA, The chemotaxonomy of actinobacterias. In A. Dietz and D.W. Thayer (eds.), Actinobacteria taxonomy. Special publications No. 6. Society for Industrial Microbiology, Arlington, USA, 1980; 227- 291.
Lee MY, Myeong JS, Park HJ, Han K, and Kim ES, Isolation and partial characterization of a cryptic polyene gene cluster in Pseudonocardia autotrophica. J Ind Microbiol Biotechnol, 2006; 33(2): 84-87.
Gupta PB, Onder TT, Jiang GZ, Tao K, Kuperwasser C, Weinberg RA, and Lander ES, Identification of selective inhibitors of cancer stem cells by high-throughput screening. Cell, 2009: 138(4): 1–15.
Bentley SD, Chater KF, Cerdeno-Tarraga AM, Challis GL, Thomson NR, James KD, Harris DE, Quail MA, Kieser H, Harper D, Bateman A, Brown S, Chandra G, Chen CW, Collins M, Cronin A, Fraser A, Goble A, Hidalgo J, Hornsby T, Howarth S, Huang CH, Kieser T, Larke L, Murphy L, Oliver K, O’Neil S, Rabbinowitsch E, Rajandream MA, Rutherford K, Rutter S, Seeger K, Saunders D, Sharp S, Squares R, Squares S, Taylor K, Warren T, Wietzorrek A, Woodward J, Barrell BG, Parkhill J, and Hopwood D, Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2). Nature 417, 2002; 6885:141-147.
McLeod MP, Warren RL, Hsiao WL, Araki N, Myhre M, Fernandes C, Miyazawa D, Wong W, Lillquist AL, Wang D, Dosanjh M, Hara H, Petrescu A, Morin RD, Yang G, Stott JM, Schein JE, Shin H, Smailus D, Siddiqui AS, Marra MA, Jones SJ, Holt R, Brinkman FS, Miyauchi K, Fukuda M, Davies JE, Mohn WW, and Eltis LD, The complete genome of Rhodococcus sp. RHA1 provides insights into a catabolic powerhouse. Proc Natl Acad Sci U S A, 2006; 103(42): 15582–15587.
Omura S, Ikeda H, Ishikawa J, Hanamoto A, Takahashi C, Shinose M, Takahashi Y, Horikawa H, Nakazawa H, Osonoe T, Kikuchi H, Shibai T, Sakaki Y, and Hattori M, Genome sequence of an industrial microorganism Streptomyces avermitilis: Deducing the ability ofproducing secondary metabolites. Proc Natl Acad Sci USA , 2001; 98(21): 12215–12220.
Beman BL, Actinomycete pathogen. In Goodfellow M，Mordarski M. and Williams S.T.(eds.). The biology of the actinomyces. Academic press, London, UK, 1983; 457-480.
Brown JM, Steigerwalt AG, Morey RE, Daneshvar MI, Romero LJ, and McNeil MM, Characterization of clinical isolates previously identified as Oerskovia turbata: proposal of Cellulosimicrobium funkei sp. nov. and emended description of the genus Cellulosimicrobium. Int J Syst Evol Microbiol, 2006; 56: 801-804.
Petkar H, Li A, Bunce N, Duffy K, Malnick H, and Shah JJ, Cellulosimicrobium funkei: first report of infection in a nonimmunocompromised patient and useful phenotypic tests for fferentiation from Cellulosimicrobium cellulans and Cellulosimicrobium terreum. J Clin Microbiol, 2011; 49(3): 1175-8.