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Introgression the SALTOL QTL into Q5DB, the Elite Variety of Vietnam Using Marker- Assisted - Selection (MAS)
American Journal of BioScience
Volume 1, Issue 4, November 2013, Pages: 80-84
Received: Oct. 1, 2013; Published: Nov. 30, 2013
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Authors
Luu Thi Ngoc Huyen, Molecular Biology Division, Agricultural Genetics Institute, Pham Van Dong Road, Tuliem, Hanoi, Vietnam
Luu Minh Cuc, Molecular Biology Division, Agricultural Genetics Institute, Pham Van Dong Road, Tuliem, Hanoi, Vietnam
Le Huy Ham, Molecular Biology Division, Agricultural Genetics Institute, Pham Van Dong Road, Tuliem, Hanoi, Vietnam
Tran Dang Khanh, Molecular Biology Division, Agricultural Genetics Institute, Pham Van Dong Road, Tuliem, Hanoi, Vietnam
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Abstract
Rice is a main food crop in Vietnam and plays a key role in economic growth in this country. However, its production and cultivating areas are severely affected from the threats of devastation caused by the rise of sea level. In this study, we have focused on developing new rice lines with salinity tolerance and high yield by applying markers assisted selection (MAS). Total of 21 primers in the Saltol QTL region were checked with the two parents varieties to identify polymorphic primers for screening the Saltol QTL region of the breeding populations. The individual plants in BC1, BC2, and BC3 generations of the Q5DB/FL478 were analyzed to evaluate the introgression of Saltol fragment into Q5DB cultivar. After screening of 3 BC generations, the best individual plants of BC3F1 of the plant numbers QF-3-1, QF-3-2, QF4-3-3, QF6-3 with almost the recipient alleles were selected. Conventional breeding was developed on BC3F2 for selection of the new salt tolerance rice lines with high yield, resistance to the biotic stress. The salinity tolerance of the new lines was also performed using the standard system of screening.
Keywords
Q5DB, Marker Assisted Selection, Rice, Saltol- QTL
To cite this article
Luu Thi Ngoc Huyen, Luu Minh Cuc, Le Huy Ham, Tran Dang Khanh, Introgression the SALTOL QTL into Q5DB, the Elite Variety of Vietnam Using Marker- Assisted - Selection (MAS), American Journal of BioScience. Vol. 1, No. 4, 2013, pp. 80-84. doi: 10.11648/j.ajbio.20130104.15
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