Influence of Exogenous Oxidative Stress on Escherichia Coli Cell Growth, Viability and Morphology
American Journal of BioScience
Volume 1, Issue 4, November 2013, Pages: 59-62
Received: Oct. 23, 2013; Published: Nov. 10, 2013
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Authors
Md. Sakil Munna, Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh
Ifra Tun NUR, Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh
Tasmina Rahman, Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh
Rashed Noor, Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh
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Abstract
The present study attempted to determine the influence of exogenous oxidative stress on the cell viability of Escherichia coli. In this regard, 3mM hydrogen peroxide (H2O2) was added to the late log phase of E. coli culture, and afterwards the phenotype, cell morphology and the ability to form colony forming units (CFU) on agar plates were examined. As expected, a quick phenotypic suppression as well as a rapid decline in viable and culturable cell numbers was observed at the mid-stationary phase as compared to control. Interestingly, a large mass of cell aggregates was noticed upon addition of H2O2. Thus the current investigation corroborated the previous findings and further added to the existing knowledge on oxidative stress events in E. coli.
Keywords
Escherichia Coli, Oxidative Stress, Hydrogen Peroxide (H2O2), Cell Culturability, Cell Death
To cite this article
Md. Sakil Munna, Ifra Tun NUR, Tasmina Rahman, Rashed Noor, Influence of Exogenous Oxidative Stress on Escherichia Coli Cell Growth, Viability and Morphology, American Journal of BioScience. Vol. 1, No. 4, 2013, pp. 59-62. doi: 10.11648/j.ajbio.20130104.12
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