Antibacterial Activity of Aqueous and Alcoholic Extracts of Capsella Bursa against Selected Pathogenic Bacteria
American Journal of BioScience
Volume 1, Issue 1, May 2013, Pages: 6-10
Received: Apr. 27, 2013; Published: May 2, 2013
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Authors
Raad N. Hasan, Department of biology. Collage of science , University of Al-Mustansiryah, Baghdad, Iraq
Mun’im R. Ali, Department of biology. Collage of science , University of Al-Mustansiryah, Baghdad, Iraq
Sinna M. Shakier, Ministry of health \ Teaching laboratory, Baghdad, Iraq
Anfal M. Khudhair, Department of biology. Collage of science , University of Al-Mustansiryah, Baghdad, Iraq
Moayad S. Hussin, Department of biology. Collage of science , University of Al-Mustansiryah, Baghdad, Iraq
Yasamin A. Kadum, Department of biology. Collage of science , University of Al-Mustansiryah, Baghdad, Iraq
Ali I. Mohammed, Department of biology. Collage of science , University of Al-Mustansiryah, Baghdad, Iraq
Ahmaed A. Abbas, Department of biology. Collage of science , University of Al-Mustansiryah, Baghdad, Iraq
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Abstract
Antibacterial activity of ethanolic and aqueous extracts of Capsella Bursa were carried out against eight different species of bacteria, Gram-positive Staphylococcus aureus and Enterococcus fecalis and Gram-negative Escherichia coli, Proteus vulgaris, Serratia marcescens,Acinitobacter bumani, Klebsiella pneumoniae and Pseudomonas aeruginosa. It is active antibacterial only against gram-negative bacteria. The ethanolic and aqueous extract from the same plant showed different activities; the aqueous extract (hot) showed the same or greater activity than the ethanolic extract. by disc diffusion, hot aqueous extract 2000 and 3000 µg/ml inhibition zone was observed against five gram negative pathogens in almost similar pattern . Ethanolic extract was active only against Ps.aeruginosa and K. pneumoniae. The MIC and MBC values of the ethanolic and aqueous extract of Capsella Bursa was active against most bacteria. All isolates were tested by different concentration of sub-MIC of aqueous and ethanolic extracts give different activities of these concentrations to inhibit or omitting the ability of those isolates to produce virulence factors (DNase, Haemolysin production and Lipase production). Finally, the Broth dilution assay showed larger activity than the disc diffusion method.
Keywords
Antibacterial Activity, Capsella Bursa, Bacteria
To cite this article
Raad N. Hasan, Mun’im R. Ali, Sinna M. Shakier, Anfal M. Khudhair, Moayad S. Hussin, Yasamin A. Kadum, Ali I. Mohammed, Ahmaed A. Abbas, Antibacterial Activity of Aqueous and Alcoholic Extracts of Capsella Bursa against Selected Pathogenic Bacteria, American Journal of BioScience. Vol. 1, No. 1, 2013, pp. 6-10. doi: 10.11648/j.ajbio.20130101.12
References
[1]
Allero, AA. and Afolayan, AJ. Antimicrobial activity of Solanum tomentosum. Afr. J. Biotechnol. 5: 369-372. (2006).
[2]
Atlas, R.M.; Parks, L.C. and Brown, A.E. (1995).laboratory Manual of Experimental Microbiology. Mosby compa-ny .Missouri. U.S.A.
[3]
Aksoy, A.; Dixon, J.M. and. Hale, W.H.G . Capsella bur-sa-pastoris (L.) Medikus (Thlaspi bursa-pastoris L., Bursa bursa-pastoris (L.) Shull, Bursa pastoris (L.) Weber).Journal of Ecology .86 (1) :171–186.(1998).
[4]
Bibitha, B.; Jisha, V.K.; Salitha, C.V.; Mohan, S. and Valsa, A.K. Antibacterial activity of different plant extracts. Short Communiaction. Indian J. Microbiol. 42: 361-363. (2002).
[5]
Choi, H. S. and Lee, M. S. The effect of dispersion medium on intensity of volatile flavor components and recovery of essential oil from Capsella bursa-pastoris by steam distillation. Korean J. Food Sci. Technol., 28, 827-833.( 1996).
[6]
Cndy, L.A. the antibacterial alternative: The natural guide to fighting infection and maintaining a health immune system. inner traditional.bear&company .132, (2000).
[7]
Kianbakht, S. and Jahaniani, F. Evaluation of Antibacterial Activity of Tribulus terrestris L. Growing in Iran. Iranian J. of Pharma. & Therap., 2(1):22-24. (2003).
[8]
Lans, C.; Turner, N.; Khan, T.; Brauer, G. and Boepple, W. Ethno veterinary medicines used for ruminants in British Columbia, Canada. 3:11. (2007).
[9]
Parekh, J.; Nair, R.and Chanda, S. Preliminary screening of some folkloric plants from Western India for potential anti-microbial activity. Indian J. Pharmacol. 37: 408-409. (2005).
[10]
Park, C.J.; Park, C.B.; Hong, S.S.; Lee, H.S.; Lee, S.Y. and Kim, S.C. Characterization and cDNA cloning of two gly-cine-and histidin-rich antimicrobial peptides from the roots of shepherd´s purse, Capsella bursa-pastor. Plant Molecular Biology, 44, 2, 187-197. (2000).
[11]
Pretorius, C.J. and Watt, E. Purification and identification of active components of Carpobrotus edulis L. J. Ethnopharm. 76:87-91. (2001).
[12]
Sener, B. and Orhan, I. Discovery of drug candidates from some Turkish plants and conservation of biodiversity. Pure Appl. Chem., 77(1) : 53–64, (2005).
[13]
Taylor, J.L.S.; Rabe, T.; McGraw, L.J.; Jager, A.K. and van Staden, J. Towards the scientific validation of traditional medicinal plants. Plant Growth Regul . 34: 23-37. (2001).
[14]
Wikipedia .cited by wikipe-dia.org/wiki/Capsella_bursa-pastoris.2008
[15]
Ratheesh,M.and Helen,A.(2007).anti inflammatory of Ruta graveolens .L.on carrageenan induced paw edema in wistar male rats .Africian .J.Biotechnology .6(10):1209-1211
[16]
Ekwenye, U. N. and Njkou, N. U. (2006). Antibacterial effect of Phyllanthus nirui (Chanca piedra) on three Enterobathogens in man. International Journal of Molecular Medicine and Advance Science , 2(2):184-189.
[17]
Hassan, A.; Rahman, S.; Deeba, F. and Mahmud, S.(2009). Antimicrobial activity of some plant extracts having hepa-toprotective effects. Journal of Medicinal Plants Research, 3(1): 020-023.
[18]
Atlas, R.M., L.C. Parks and A.E. Brown, 1995. Laboratory Manual of Experimental Microbiology. Mosby-Year Book, Inc., St. Louis.
[19]
Lorian, V. Antibiotics in laboratory medicine. Baltimore: The Williams and Wilkins Company; 1991. p. 1-51.
[20]
National Committee for Clinical Laboratory Standards NCCLS (2000).Methods for Dilution antimicrobial susceptibility tests for bacteria that grow aerobically: approved standards- fifth edition. NCCLS document M7-A5. NCCLS: Wayne, PA, USA.
[21]
Cowan MM. Plant products as antimicrobial agents. Clinical Microbiology Review 1999; 12(4): 564-582.
[22]
Zennie TM, Ogzewalla D 1977. Ascorbic acid and vitamin C content of edible wild plants of Ohio and Kentucky. Econ Bot 31: 76-79.
[23]
Kweon MH, Kwak JH, Ra KS, Sung HC, Yang HC 1996. Structural characterization of a flavonoid compound sca-venging superoxide anion radical isolated from Capsella bursapastoris. J Biochem Mol Biol 29: 423-428.
[24]
Guil-Guerrero JL, Giménez-Martínez JJ, Torija-Isasa ME 1999. Nutritional composition of wild edible crucifer species. J Food Biochem 23: 283-294.
[25]
Tsimikas S, Philis-Tsimikas A, Alexopoulos S, Sigari F, Lee C, Reaven PD 1999. LDL isolated from greek subjects on a typical diet or from American subjects on an oleatesupplemented diet induces less monocyte chemotaxis and adhesion when exposed to oxidative stress. Arterioscl Throm Vas Biol 19: 122-130.
[26]
Hammond-Kosack, K. E. & Jones, J. D. G. (1997) Annu. Rev. Plant Physiol. Plant Mol. Biol. 48, 575–607.
[27]
Oliveira AP, Pereira D, Andrade PB, Valentão P, Sousa C, Pereira JA, Bento A, Rodrigues MA, Seabra RM, Silva BM 2008. Free amino acids of tronchuda cabbage (Brassica ole-racea L. var. costata DC): Influence of leaf position (internal or external) and collection time. J Agric Food Chem 56: 5216-5221.
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