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Neurogenic Differentiation of Bone Marrow-derived Mesenchymal Stem Cells Using Neural Induction Medium: A Morphological and Histochemical Study
American Journal of Bioscience and Bioengineering
Volume 3, Issue 4-1, July 2015, Pages: 43-50
Received: Jun. 26, 2015; Accepted: Jun. 27, 2015; Published: Jul. 6, 2015
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Ahmedy E., Clinical pathology department, faculty of medicine, El Menoufia universit, Menofia, Egypt
Kandel S., Clinical pathology department, faculty of medicine, El Menoufia universit, Menofia, Egypt
Gabr H., Clinical pathology department, faculty of medicine, Kasr El Eini University, Cairo, Egypt
Rizk S., Clinical pathology department, faculty of medicine, Kasr El Eini University, Cairo, Egypt
Khalifa . K, Clinical pathology department, faculty of medicine, El Menoufia universit, Menofia, Egypt
Kamal S., Clinical pathology department, faculty of medicine, El Menoufia universit, Menofia, Egypt
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Background: Mesenchymal stem cells (MSCs) are multipotent adult stem cells present in all tissues. They are present in bone marrow, and can differentiate in vitro into neurons, glial cells and myofibroblasts . MSCs have been proposed as sources of stem cells for regeneration of the CNS. Thus, one of the goals of regenerative medicine is to regenerate damaged brain tissue and spinal cord by harnessing the power of stem cells to initiate neurogenesis in damaged areas of the brain. Objective: The aim of this work is to study in-vitro induced neurogenesis using MSCs as model of stem cells. Methodology : Bone marrow-MSCs were isolated, expanded and passaged. MSCs were identified using morphology and flowcytometric analysis. Co-expression of Oct ¾ was done. MSCs were induced to neural lineage using Neural Induction Media (NIM) : a cocktail of retinoic acid dissolved in DEMSO, recombinant human Fibroblast Growth Factor (FGF) basic, recombinant human Epidermal Growth Factor (EGF) and Insulin-like Growth Factor I (IGF-I) . Neural induction was verified morphologically, and immunologically using GFAP positivity and nestin expression. Results: BM-MSCs express CD44 and OCT ¾ which decrease with age. MSCs induced with NIM show morphological changes consistent with neurogenesis, positive GFAP and nestin expression as compared to the uninduced cells. Conclusion: MSCs isolated from bone marrow aspirate and can be differentiated into GFAP positive neural cells.
Mesenchymal Stem Cells, GFAP, Retinoic Acid, Neurogenesis
To cite this article
Ahmedy E., Kandel S., Gabr H., Rizk S., Khalifa . K, Kamal S., Neurogenic Differentiation of Bone Marrow-derived Mesenchymal Stem Cells Using Neural Induction Medium: A Morphological and Histochemical Study, American Journal of Bioscience and Bioengineering. Special Issue: Stem Cells for Neuro-Regeneration: Where Do We Stand. Vol. 3, No. 4-1, 2015, pp. 43-50. doi: 10.11648/
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