American Journal of Bioscience and Bioengineering
Volume 7, Issue 1, February 2019, Pages: 22-27
Received: Mar. 3, 2019;
Accepted: Apr. 9, 2019;
Published: Apr. 29, 2019
Views 745 Downloads 144
Ismail Haruna Yahaya, Department of Microbiology, Faculty of Science, University of Maiduguri, Maiduguri, Nigeria
Riskuwa-Shehu Maryam Lami, Department of Microbiology, Faculty of Science, Usmanu Danfodiyo University, Sokoto, Nigeria
Allamin Ibrahim Alkali, Department of Microbiology, Faculty of Science, University of Maiduguri, Maiduguri, Nigeria
Ahmad Ali Farouq, Department of Microbiology, Faculty of Science, Usmanu Danfodiyo University, Sokoto, Nigeria
Cathiong Stephen Abakwak, Department of Microbiology, Faculty of Science, University of Maiduguri, Maiduguri, Nigeria
Contamination of soil with hydrocarbons is gradually increasing since oil explorations began in Nigeria. With claims of new exploration sites, widespread contamination is possible in many parts of the country in the near future. In view of that, this study aimed to evaluate the growth and phytoremediation potentials of Vigna unguiculata (Cowpea) and Vigna subterrenean (Bambara nut) in hydrocarbon contaminated soil. The study involved a field experiment conducted in a botanical garden under irrigation. The plants were grown on 0%, 5%, and 10% (v/w) used engine oil contaminated soil in plastic bowls. Percentage emergence of V. unguiculata was between 20% and 100%, while that of V. subterrenean was between 50% and 60%. Phytotoxicity studies showed that the oil was toxic to both plants especially at 10% oil concentration and V. unguiculata was more tolerant than V. subterranean. Microbial analyses revealed more bacterial cells as period of treatment increased presumably due to root exudation in the rhizosphere. Eighteen bacterial isolates were identified to belong to Bacillus, Acinetobacter, Klebsiella, Pseudomonas, Enterobacter, Micrococcus, Serratia, Proteus and Staphylococcus genera. All the isolates were found to utilize used engine oil as sole carbon and energy source. The plants and associated bacterial consortium therefore, could be used as important tools in reclaiming soil contaminated with low levels of used engine oil.
Ismail Haruna Yahaya,
Riskuwa-Shehu Maryam Lami,
Allamin Ibrahim Alkali,
Ahmad Ali Farouq,
Cathiong Stephen Abakwak,
Biostimulation Potentials of Vigna Species (L.) in Hydrocarbon Impacted Soil, American Journal of Bioscience and Bioengineering.
Vol. 7, No. 1,
2019, pp. 22-27.
Blodgett, W. C. 2001. Water Soluble Mutagens Produced During the Bioremediation of Oil Contaminated Soil. Florida Scientist.60 (1): 28-36.
Ugoh, S. C. and Moneke, L. U., 2011. Isolation of Bacteria from Engine Oil Contaminated soils in Auto mechanic workshops in Gwagwalada, Abuja, FCT Nigeria. Academia Arena 3(5):28-33.
Udebuani, A. C., Okoli, C. G., Okoli, I. C., Nwigwe, H. C. and Ozoh, P. T. E. 2011. Assessments of the Volume and Disposal Methods of Spent Engine Oil Generated in Nekede Mechanic village, Owerri, Nigeria. Reportand Opinion 3(2):31-36.
Ajayi, D. D., Ikporukpo, C. O., 2005. An analysis of Nigeria’s environmental vision 2010. J. Environ. Policy Plan. 7, 341–365.
Altas, R. M. and Bartha, R., 1993. Stimulated biodegradation of oil slicks using oleophilic fertilizers.” Environmental Science Technology.7:538-541.
Udom, B. E. and Nuga, B. O. 2011. Characterization of Soil Health Using Microbial Community and Maize Germination as Bioindicatorsin Oil-Contaminated Soil. Journal of Advances in Developmental Research 2 (2):191-197.
Ite, A. E., Ibok, U. J., Ite, M. U. and Petters, S. W. 2013. Petroleum Exploration and Production: Past and Present Environmental Issues in the Nigeria’s Niger Delta. Nature 1, 78–90.
Sam, K., Coulon, F. and Prpich, G. 2017. Management of petroleum hydrocarbon contaminated sites in Nigeria: Current challenges and future direction. Land use policy; 64:133-144.
Nwaichi, E. O, Frac, M, Nwoha, P. A. and Eragbor, P. 2015. Enhanced phytoremediation of Crude oil-polluted soil by four plant species: effect of inorganic and organic bioaugumentation. Int. J. Phytoremediation 17(12): 1253-1261.
Lee, K., Doe, K. G., Lee, L. E. J., Suidan, M. T., Venosa, A. D. 2001. Remediation of an oil contaminated experimental freshwater wetland: Habitat recovery and toxicity reduction. Proceedings of the 2001 International Oil Spill Conference. American Petroleum Institute, Washington, DC.
Howell, J. A., W. H. Eshbaugh, S. Guttman and E. Rabakonandrianina, 1994. Common names given to bambara groundnut (Vigna subterranea) in Madagascar. Econ. Bot., 48:217-221.
Johnson, C. R and Scow, K. M. 1999. Effect of nitrogen and phosphorous addition on phenanthrene biodegradation in four soils. Biodegradation 10, 43-50.
Dakora, F. D. and Phillips, D. A. 2002. Root Exudates as Mediators of Mineral Acquisition in Low-Nutrient Environments. Plant and Soil 245: 35–47.
Brink, M, Romalemana, G. M, Sibuga, K. P. 2006. Vigna subterrenean (L) Verde. Record from Protobase.
Ibrahim, S. and Nafi’u, S. A. 2017. In vitro Effects of Tannery Effluents on Seed Germination and Growth Performance of Maize (Zea mays), Spinach (Spinacia caudatus) and Lettuce (Lactuca sativa). International Journal of Applied Biological Research; 8(1): 160–173.
Ismail, H. Y., Ijah, U. J. J., Riskuwa, M. L. and Ibrahim A. A. 2014. Biodegradation of spent engine oil by bacteria isolated from the rhizosphere of legumes grown in contaminated soil. International Journal of Environment; 3(2):85-97.
Barrow, G. I. and Feltham, K. A. 1993. Cowan and Steel’s Manual for Identification of Medical Bacteria. 3rd edition. Cambridge University Press, London.
Holt, J. G., Krieg, N. R., Sneath, P. H. A., Stately, J. T. and Williams, St. 1994. Bergey’s manual of determinative bacteriology, 9th ed, Baltimore, Williams and Wilkins.
Osuagwu, A. N. and Iwuoha, C. L. 2015. Assessment of Spent Engine Oil on the Germination Ability of Cajanus cajan, Vigna subterranean and Phaselous vulgaris. International Conference on Advances in Agricultural, Biological & Environmental Sciences (AABES-2015) July 22-23, 2015 London (UK).
Kathi, S. and Khan, A. B. 2011. Toxicity of spent oil contaminants on Dolichos lablab L. and Abelmoschus esculentus L. The Ecoscan; 4(1):133-136.
Agbogidi, O. M. 2010. Response of six cultivars of cowpea Vigna unguiculata ((L) (Walp) to spent engine oil. African Journal of food science and technology 1 (6): 139-142.
Cunningham, S. D., Anderson, T. A., Schwab, P. A. and Hsu, F. C. 1996. Phytoremediation of Soils Contaminated with Organic Pollutants. Advances in Agronomy, 56:55-114.
Schnoor, J. L., Licht, L. A., McCutcheon, S. C., Wolfe, N. L. and Carreira, L. H. 1995. Phytoremediation of organic and nutrient contaminants. Environmental Science and Technology 29 (7): 318-323.
Erickson, L. E., Davis, L. C. and Muralidharan, N. 1995. Bioenergetics and Bioremediation of Contaminated Soil. Thermochimica Acta.250: 353-358.
Aprill, W. and Sims, R. C. 1990. Evaluation of the Use of Prairie Grasses for Stimulating Polycyclic Aromatic Hydrocarbon Treatment in soil. Chemosphere 20:253-265.
Philips, A. L. 2008. The relationship between plants and their root-associated microbial communities in hydrocar-bon phytoremediation systems. Saskatoon: University of Saskatchewan; 2008. 144 p.
Hrynkiewicz, K., Baum, C., Niedojadło, J. and Dahm, H. 2009. Promotion of Mycorrhiza Formation and Growth of Willows by the Bacterial Strain Sphingomonassp. 23L on Fly Ash. Biology and Fertility of Soil 45:385–394.
Brimecombe, M. J., De Leij, F. A. M. and Lynch, J. M. 2007. Rhizodeposition and Microbial Populations. In: R. Pinton., Z. Varanini. and P. Nannipieri. (eds) The Rhizosphere: Biochemistry and Organic Substances at the Soil-plant Interface. CRC Press, Taylor & Francis Group, New York, pp. 73–109.