American Journal of Bioscience and Bioengineering
Volume 4, Issue 3, June 2016, Pages: 34-40
Received: Apr. 14, 2016;
Accepted: Apr. 25, 2016;
Published: May 10, 2016
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Hinawi A. M. Hassanin, State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, China
Wanmeng Mu, State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, China
Marwa Y. F. Koko, State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, China
Tao Zhang, State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, China
Ammar Alfarga, State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, China
Mandour H. Abdelhai, State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, China
Bo Jiang, State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, China
Allitol is an alcohol monosaccharide, is a reduction of D-psicose. It is functions as a cross linking of D- and L-hexoses. It existed in too small quantities in commercial sugars and is difficult to synthesize by using chemical methods. It has a hypoglycemic function, and can use as Laxative in treating of constipation, which can exploit in production of diabetes drugs. The present report investigates about the production of allitol by ribitol dehydrogenase (RDH), its action of the enzyme through homology and molecular docking studies. We have investigated ribitol dehydrogenase (RDH) from providencia alcalifaciens RIMD 1656011. The protein sequence of RDH was conducted for homology modeling through Swiss model. 3D structure revealed was docked with NAD+ and D-psicose using AutoDock Vina software version 5.6. The results of homology modeling and docking studies revealed that the conserved residues of RDH were Tyr 153, Tyr 92, Ser 17 and Lys157 with NAD+, while conserved residues with D-psicose were GLN67 and ASP61. NAD+ has good interaction with RDH showing grid score of -49.84, which is a good score for binding.
Hinawi A. M. Hassanin,
Marwa Y. F. Koko,
Mandour H. Abdelhai,
Molecular Modeling and Docking of Ribitol Dehydrogenase Exploring Enzyme NAD+ and D-psicose Interaction, American Journal of Bioscience and Bioengineering.
Vol. 4, No. 3,
2016, pp. 34-40.
Ayers, B. J., Hollinshead, J., Saville, A. W., Nakagawa, S., Adachi, I., Kato, A., Izumori, K., Bartholomew, B., Fleet, G. W., Nash, R. J. (2014). "Iteamine, the first alkaloid isolated from Ite avirginica L. inflorescence. "Phytochemistry 100 :126-131.
Wu, S. H., Luo, X. D., Ma, Y. B., Liu, J. K., Wu, D. G., Zhao, B., Lu, Y., Zheng, Q. T. (2000). "Two Novel Secoergosterols from the Fungus Tylopilus p lumbeoviolaceus. "Journal of natural products 63(4): 534-536.
Takeshita, K., Ishida, Y., Takada, G., Izumori, K. (2000). "Direct production of allitol from D-fructose by a coupling reaction using D-tagatose 3-epimerase, ribitol dehydrogenase and formate dehydrogenase. " Journal of bioscience and bioengineering 90(5): 545-548.
Zhu, Y., Li, H., Liu, P., Yang, J., Zhang, X., Sun, Y. (2015). "Construction of allitol synthesis pathway by multi-enzyme coexpression in Escherichia coli and its application in allitol production. "Journal of industrial microbiology & biotechnology 42(5): 661-669.
Oosaka, K. (2009). "Possibility as monosaccharide laxative of rare sugar alcohols." Yakugaku Zasshi1 29(5): 575-580.
Izumori, K. (2006). "Izumoring: a strategy for bioproduction of all hexoses. "Journal of biotechnology 124(4): 717-722.
Ballard, J. M. and Stacey, B. E. (1973). "The reaction of allitol with hydrogen halides. "Carbohydrate Research 30(1): 83-89.
Moon, H. J., Tiwari, M., Jeya, M., Lee, J. K. (2010). "Cloning and characterization of a ribitol dehydrogenase from Zymomonas mobilis. "Applied microbiology and biotechnology 87(1), pp. 205-214.
Fossitt, D. D., and Wood, W. A. (1966). "Pentitol dehydrogenases of Aerobacter aerogenes. "Methods in enzymology 9: 180-186.
Muniruzzaman, S., Kunihisa, Y., Ichiraku, K., Izumori, K. (1995). "Purification and characterization of a ribitol dehydrogenase from Enterobacter agglomerans strain 221e. "Journal of fermentation and bioengineering 79: 496-498.
Adachi, O., Fujii, Y., Ano, Y., Moonmangmee, D., Toyama, H., Shinagawa, E., Matsushita, K. (2001). "Membrane-bound sugar alcohol dehydrogenase in acetic acid bacteria catalyzes L-ribulose formation and NAD-dependent ribitol dehydrogenase is independent of the oxidative fermentation. "Bioscience, biotechnology, and biochemistry 65: 115-125.
Taylor, S. S., Rigby, P. W., Hartley, B. (1974). "Ribitol dehydrogenase from Klebsiella aerogenes. Purification and subunit structure. " Biochemical Journal 141: 693.
Kahle, C., Schneider, K. H., Giffhorn, F. (1992). "Pentitol metabolism of Rhodobacter sphaeroides Si4: purification and characterization of a ribitol dehydrogenase. " Microbiology 138: 1277-1281.
Han, W., Zhu, Y., Men, Y., Yang, J., Liu, C., Sun, Y. (2014). "Production of allitol from D-psicose by a novel isolated strain of Klebsiella oxytoca G4A4." Journal of basic microbiology 54: 1073-1079.
Hassanin, H. A., Wang, X., Mu, W., Zhang, T. and Jiang, B. (2016). "Cloning and characterization of a new ribitol dehydrogenase from Providencia alcalifaciens RIMD 1656011. "Journal of the science of food and agriculture doi: 10.1002/jsfa.7589.
Koehl, P., and Levitt, M. (1999). "A brighter future for protein structure prediction. "Nature structural biology 6: 108-111.
Sánchez, R., and Šali, A. (1997). "Advances in comparative protein-structure modelling. "Current opinion in structural biology 7(2): 206-214.
Singh, P. K., and Shukla, P. (2012). "Molecular Modeling and Docking of Microbial Inulinases Towards Perceptive Enzyme–Substrate Interactions. "Indian journal of microbiology 52(3): 373-380.
Arnold, K., Bordoli, L., Kopp, J., Schwede, T. (2006). "SWISSMODEL workspace: a web-based environment for protein structure homology modeling. "Bioinformatics 22: 195–201.
Kiefer, F., Arnold, K., Kunzli, M., Bordoli, L., Schwede, T. (2009). "The SWISS-MODEL repository and associated resources." Nucleic Acids Res 37: D387–D392.
Schwede, T., Kopp, J., Guex, N., Peitsch, M. C. (2003). "SWISSMODEL: an automated protein homology-modeling server. "Nucleic Acids Res 31: 3381–3385.
Guex, N., and Peitsch, M. C. (1997). "SWISS-MODEL and the Swiss- PdbViewer: an environment for comparative protein modelling. "Electrophoresis 18: 2714–2723.
Filling, C., Berndt, K. D., Benach, J., Knapp, S., Prozorovski, T., Nordling, E., Ladenstein, R., Jornvall, H., Oppermann, U. (2002). "Critical residues for structure and catalysis in short-chain dehydrogenases/reductases. "Journal of Biological Chemistry 277: 25677–25684.
Oppermann, U., Filling, C., Hult, M., Shafqat, N., Wu, X., Lindh, M., Shafqat, J., Nordling, E., Kallberg, Y., Persson, B., Jornvall, H. (2003). "hortchain dehydrogenases/reductases (SDR): the 2002 update. "Chemico-biological interactions 143: 247–253.