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Prevalence and Socio-environmental Predictors of Cryptosporidiosis in Kebbi State, Nigeria
American Journal of Bioscience and Bioengineering
Volume 3, Issue 6, December 2015, Pages: 149-157
Received: Nov. 6, 2015; Accepted: Nov. 19, 2015; Published: Dec. 14, 2015
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Danladi Yusuf Kanya, Department of Zoology, University of Ilorin, Ilorin, Nigeria
Ugbomoiko Uade Samuel, Department of Zoology, University of Ilorin, Ilorin, Nigeria
Babamale Olarewaju Abdulkareem, Department of Zoology, University of Ilorin, Ilorin, Nigeria
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A cross-sectional study to determine the prevalence and socio-environmental factors associated with cryptosporidiosis was carried out between January to December 2012 in two communities in Kebbi state, Nigeria. Faecal specimen was collected from each participant and structured questionnaire applied. Samples were examined for Cryptosporidium by formal-ether concentration and modified Ziehl-Neelsen staining technique. A total of 2100 participants were tested, 290(13.8%) were infected with Cryptosporidium. Logistic regression analysis indicates that, in Aliero, infection was associated with: Presence younger children (Odds Ratio=1.889, P-value <0.0001, 95% Confidence Interval= 1.568-2.274), Regular hands washing (OR=0.399, P<0.0001, 95% CI=0.283-0.535), Presence of diarrhoea (OR= 2.66, P<0.0001, 95% CI= 1.733-4.100), While in Zuru, the infection was predicted by: Younger age group (OR=1.283, P= 0.004, 95% CI=1.085-1.520), Married status (OR=2.463, P=0.028, 95%CI=1.100-5.513), Lack of formal education (OR=2.993, P<0.0001,95% CI=1.872-4.786) and Farming occupation (OR=1.392, P=0.002, 95% CI=1.135-1.703). It was concluded that unhygienic behavioral variables, certain environmental and socio-demographic factors predicted the presence of cryptosporidiosis in the area. Sustainable intervention should include basic health education, access to clean water and adequate sanitation.
Cryptosporidiosis, Prevalence, Risk Factors, Kebbi State
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Danladi Yusuf Kanya, Ugbomoiko Uade Samuel, Babamale Olarewaju Abdulkareem, Prevalence and Socio-environmental Predictors of Cryptosporidiosis in Kebbi State, Nigeria, American Journal of Bioscience and Bioengineering. Vol. 3, No. 6, 2015, pp. 149-157. doi: 10.11648/
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Gambo, A., Inabo, H.I., Aminu, M. (2014). Prevalence of Cryptosporidium Oocysts among children with acute gastroenteritis in Zaria, Nigeria. Bajopas 7(2): 155 – 159.
Fletcher, S.M., Stark, D., Harkness, J. and Ellis J., (2012). Enteric protozoa in the developed world: a public health perspective. Clinical Microbiology Reviews 25(3), 420-449.
Karanis P, Kourenti C, and Smith H. (2007). Waterborne transmission of protozoan parasites: a worldwide review of outbreaks and lessons learnt. J. Water Health5, 1-38.
Chunge, R.N., Simwa, J.M., Karumba, P.N., Kenya, P.R., Kinoti, S.N., Muttunga, J., and Nagelkerke, N. (1992). Comparative etiology of childhood diarrhoeain Kakamega and Kiambu districts, Kenya. East Afr Med J., 69(8), 437-41.
Newman, R. D., Sears, C.L., Moore, S.R., Nataro, J.P., et al., (1999). Longitudinal study of Cryptosporidium infection in children in northeastern Brazil. J. INFECT. Dis.; 180, 167-75.
Periera, S.J., N.E. Ramirez, L. Xiao, and L.A. Ward. (2002). Pathogenesis of human and bovine Cryptosporidium parvum in gnotobiotic pigs. Journal of Infectious Diseases 186: 715-718.
Elsser, K.A., Moricz, M., Proctor, E.M., (1986). Cryptosporidium infections: a laboratory survey. CMAJ. 135(3), 211-3.
Navarrete, S., Stetler, H.C., avila, C., Garcia aranda, J.A., and Santos-preciado, J.I. (1991). An outbreak of Cryptosporidium diarrhea in a pediatric hospital. pediatr Infect dis J. 10(3), 248-50.
Siwilia J, phiri IG, Vercruysse j, Goma f, Gabriels S, Claereboute, Geurden T. (2007). Asymptomatic cryptosporidiosis in Zambian dairy farm workers and their household members.Trans R Soc Trop Med Hyg. 101(7), 733-4.
Hunter, P.R., Chalmers R, M., Syed, Q., Hughes, L.S., Woodhouse. S., and Swift, L. (2003). Foot and mouth disease and cryptosporidiosis: possible interaction between two emerging infectious diseases. Emerg Infect dis., 9(1), 109-12.
Kelly, p., Baboo, K.S., Ndubani, p., Nchito, M., Okeowo, N.P., Luo N.P., feldmanra, farthing MJ. (1997). Cryptosporidiosis in adults in Lusaka, Zambia and its relationship to oocyst contamination of drinking water. J Infect dis. 176(4), 1120-1123.
Molbak, K., Aaby, P., Hojlyng, N., and Da Siwa, A.P.J. (1994). Risk factors for Cryptosporidium diarrhoea in early childhood: a case control study from Guinea-Bissau, West africa. Am J epidemiol., 139, 734-40.
Nizeyi, J.B., Sebunya, D., Dasilva, A.J., Cranfield, M.R., pieniazek, N.J., Graczyk, T.K. (2002). Cryptosporidiosis in people sharing habitats with free-ranging mountain gorillas (Gorilla gorillaberingei), Uganda.Am J Trop Med Hyg., 66(4), 442-4.
Nichols G.L., Chalmers R.M., Sopwith W., Regan M., Hunter C.A., Grenfell P., Harrison F. and Lane C. (2006).Cryptosporidiosis: a report on the surveillance and epidemiology of Cryptosporidium infection in England and Wales. Drinking Water Directorate Contract Number DWI 70/2/201. Drinking Water Inspectorate, UK. Pp142.
Cama, V.A., Bern, C., Robert, s. J., Cabrera L, et al., (2008). Cryptosporidium species and subtypes and clinical manifestations in children, Peru. Emerg. Infect. Dis., 14 (10), 1567-74.
Molloy SF, smith HV, Kirwan P, Nichols AB, et al., (2010). Identification of a high diversity of Cryptosporidium species genotypes and subtypes in a pediatric population in Nigeria. Am J. Trop. Med. Hyg., 82 (4), 608-13.
Molloy, S. F., Tanner, J., Kirwan, P., Asaolu, S. O., Smith, H. V., Nicholas, R. A. B., Connelly, L., and Holland, C. V. (2011). Sporadic Cryptosporidium infection in Nigerian children: Risk factors with species identification. Epidemiol Infect.139, 946-948.
National Population Commission of Nigeria, 2006.
Lindo, F. L., Levy, V. A., Baum, M. K., and Palmer, C. J. (1998). Epidemiology of giardiasis and cryptosporidiosis in Jamaica. Am. J. Trop. Med. Hyg., 59 (5), 717 -721.
Cheesbrough, M., 2005: District Laboratory Practice. 2nd edition, Part 1, Cambridge University press. Pp 236 -239.
Maikai, B. V., Umoh, J. U., Lawal, I. A., Kudi, A. C., Ejimbi, C.L and Xiao, L. (2012). Molecular characterizations of Cryptosporidium, Giardia and Enterocytozoan in humans in Kaduna State, Nigeria. Exp. Parastl131, 452-456.
Banwat, E.B., Egah, D.Z., Onile, B.A., Angyo, I. A., and Audu I. S. (2003). Prevalence of Cryptosporidium infections among under nourished children in Jos Central Nigeria. Niger J Postgrd Med 10: 84-87.
Nwokediuko, S. C., Bojuwoye, B. J and Onyekekwe, B. (2002). Apparent rarity of cryptosporidiosis in human immunodeficiency virus (HIV) related diarrhea in Enugu, South-Eastern Nigeria. Niger. J Postgrad Med, 9, 70-73.
Banwat, E.B., Egah, D.Z., Onile, B.A. and Datong, P. R. (2004). Cryptosporidium infection in undernourished children with HIV/AIDS in Jos, Nigeria. Annal of African Medicine, 3(2), 80-82.
Adesiji, Y.O., Lawal, R.O., Taiwo, S.S., Fayemiwo, S.A., and Adeyoba, O. A., 39(2007). Cryptospaidiosis in HIV infected patients with diarrhea in Osun State, South Western Nigeria. Europ. J. Gen. medi, 119-122.
O’Donoghue, P.J. (1995) Cryptosporidium and cryptosporidiosis inman and animals. Int. J. Parasitol. 25, 139–195.
Zaidah, A. R., Chan, Y. Y., Asma, H. S., Abdullah, S., Nurshalindawati, A. R. and Saleh, M. (2008). Detection of Cryptosporidium parvum in HIV – infectioed patients in Malaysia using a molecular approach. Southeast Asian J. Trop Med and Pub Health, 39, 511-516.
Oyerinde J.P.O., Odugbemi T., Benson R.I., Alonge A.A., Roberts J.I.K. (1989). Investigations of Cryptosporidium in relation to other intestinal parasites at the Lagos University Teaching, Lagos. West Afr J Med 8: 264-269.
Nkanginieme, K.E.O., Chira, F.W. and Oruamabo, RS. (1996). Cryptosporidiosis in undernourished under five children with diarrhoea at the University of Port Harcourt Teaching Hospital in Nigeria. Niger Postgrad Med J, 3, 5-9.
Nwabuisi, C. (2001). Childhood cryptosporidiosis and intestinal parasitosis in association with diarrhoea in Kwara state, Nigeria. West Afri J Med., 20 (2), 165-8.
Inyang-Etoh, P.C., N.G. Etim, F.M. Useh, C.E.J. Udiong, and A.W. Essien (2007).Cryptosporidiosis and Infantile Diarrhoae in Calabar, Nigeria. J. Med. Sc. 7(8): 1325-1329.
Tumwine, J. K., Kekithnwa, A., Nabukeera, N., Akiyoshi, D. E. R., Rich, S. M., Widmer, G., Feng, X and Tzipori, S. (2003). Cryptosporidium parvum in children with diarrhea in Mulago Hospital, Kampala, Uganda. Am. J. Trop. Hyg., 68(6)710-715.
Abdel-messih, I. A., Wierzba, T. F., Abu-Elyazed, R., Ibrahim, A.F., Ahmad, S. F., Kamal, K., Sanders, J., Frenck, R. (2005). Diarrhea associated with Cryptosporidium parvum among young children of the Nile River Delta in Egypt. J. Trop Pediatr., 51(3), 154-159.
Gatei, W., Wamae, C.N., Waruru, A., Mulinge, E., Waithera, T., Ltatika, S. M., Kamwati, S. K., Revathi, G., and Hart, C. A. (2006). Cryptosporidiosis: Prevalence, Genotype analysis and symptoms associated with infections in children in kenya. Am Soc. Trop Med and Hyg. 75(1), 78-82.
Bello, J., Nunez, F. A., Gonzalez, O. M., Fernandez, R., Almirall, P., and Escobedo, A. A. (2011). Risk factors for Giardia infection among hospitalized children in Cuba. Annals of Tropical Medicine & Parasitology, 105 (10), 57-64.
Pelayo, L., Nun Ez, F.A., Rojas, L., Wilke, H., Furuseth Hansen, E., Mulder, B., Gjerde, B. and Robertson, L. (2008).Molecular and epidemiological investigations of cryptosporidiosis in Cuban children. Annals of Tropical Medicine & Parasitology, 102 (8), 659-669.
Beauty Omoruyi, Fredrick Matongo, Noxolo T. Nkwetshana, Ezekiel Green, Anna M. Clarke and Roland N. Ndip (2011). Environmental and demographic risk factors associated with the prevalence of Cryptosporidium infection in the Alice rural settlements of the Eastern Cape Province of South Africa: a pilot study. Rev Environ Health 26(2): 127–133.
Eyasu, T., Beyene, P., and Tekola, E. (2010). Prevalence of Giardiasis and Cryptosporidiosis among children in relation to water sources in Selected Village of Pawi Special District in Benishangul-Gumuz Region, Northwestern Ethiopia. Ethiop. J. Health Dev. 10; 24(3), 205-213.
Asaolu, S. O. and Ofoezie, I.E. (2003). The role of health education and sanitation in the control of helminth infections. Acta Tropica., 8,283-294.
Redlinger, T., Corella-Barud, V., Graham, J., Galindo, A., Avitia R. and CardenasV. (2002). Hyperendemic Cryptosporidium and Giardia in households lacking manucipal sewer and water on the united states – Mexico border. Am J Trop Med Hyg 66, 794 -798.
Nyamwange, C., Mkoji, G.M. and Mpoke, S. (2012) Cryptosporidiosis among HIV positive patients in North Rift Region of Kenya. Afri J Health Sci, 21, 92-106.
Pinheiro, I., Milton, F., Mitterofhe, A., Condé Pires, F.A., Abramo, C., Luiz Cláudio Ribeiro, L. C., Tibiriçá, S.H.C., Coimbra, E.S. (2011) Prevalence and risk factors for giardiasis and soil-transmitted helminthiasis in three municipalities of Southeastern Minas Gerais State, Brazil. Parasitol Res 108: 1123–1130.
Anim-Baidoo, I., Narh, C., Obiri, D., Ewerenonu-Laryea, C., Donkor, E. S., Adjei, A. A., and P. F. Ayeh-Kumi (2015). Cryptosporidial diarrhoea in children at a paediatric hospital in Accra, Ghana. IJTDH, 10(3): 1-13.
Pereira, M.G.C.; Atwill, E.R.; Barbosa, A.P.; Silva, S.A. and Garcia-Zapata, M.T., (2002). Intra-familial and extra-familial risk factors associated with Cryptosporidium parvum infection among children hospitalized for diarrhea in Goiânia, Goiás, Brazil. Amer. J. trop. Med. Hyg., 66, 787-793.
Norma E. Ramirez, Lucy A.Ward, Srinand Sreevatsan (2004). A review of the biology and epidemiology of cryptosporidiosis in humans and animals. Microbes and Infection 6: 773–785.
Pinheiro, I., Milton, F., Mitterofhe, A., Condé Pires, F.A., Abramo, C., Luiz Cláudio Ribeiro, L. C., Tibiriçá, S.H.C., and Coimbra, E.S. (2011). Prevalence and risk factors for giardiasis and soil-transmitted helminthiasis in three municipalities of Southeastern Minas Gerais State, Brazil. ParasitolRes, 108, 1123-1130.
Rao Ajjampus, S.S., J.R. Asirvatham, D. Muthusamy, B.P. Gladstone, O.C. Abraham, D. Mathai, H. Ward, C.Wanke, C. kang, (2007). Clinical features and risk factors associated with cryptosporidiosis in HIV infected adults in India. Indian J Med Res, 126, 553-557.
Bern, C., Ortega Y, Checkley, W., Roberts, J.M, Lescano, A.G., Cabrera, L., Verastegui, M., Black, R.E., Sterling, C. and Gilman, R.H. (2002). Epidemiologic differences between cyclosporiasis and cryptosporidiosis in Peruvian children. Emerging Infectious Diseases, 8:581-585.
Chancin-Bonilla, L., Borrios, F., and Sanchez, Y. (2008). Environmental risk factors for Cryptosporidium infection in an island from western Venezuela. Mem Inst Oswaldo Cruz, Rio de Janeiro 103(1), 45-49.
Pickford, J. (1988). Rural sanitation and development. Waterlines, 6, 2-4.
Lawlor, D.A. and Sterne, J.A.C. (2007). Socioeconomic inequalities in health. Brit Med J, 334, 963–964.
Drake, L. J. and Bundy, D.A. (2001) Multiple helminth infections in children: Impact and control. Parasitology 122, 73-81.
Hostez, P.J. and Kamath, A. (2009). Neglected tropical diseases in Sub-Saharan Africa: A review of their prevalence, distribution and disease burden. PLosNegl. Trop. Dis. 3: 412.
Ugbomoiko, U.S. V. Dalumo, I.E. Ofoezie, and Abiezue, R.N.N. (2009). Socio-environmental factors and Ascariasis infection among school-aged children in Ilobu, Osun State, Nigeria. Transc Royal Soc. Trop Med and Hyg 103, 223-22.
Ugbomoiko, U.S. I.E. Ofoezie, I.C. Okoye, and J. Heukelbach, (2010). Factors associated with urinary Schistosomiasis in two peri-urban communities in south-western Nigeria. Ann. Trop med parasitl, 104(5), 104 – 419.
Ugbomoiko, U.S. V. Dalumo, Y.K. Danladi., J. Heukelbach, and I.E. Ofoezie, (2012). Concurrent Urinary and intestinal Schistosomiasis and intestinal heminthic infections in Ilobu, Southwestern Nigeria.ActaTropica123, 16- 21.
Raschid-Sally, L. and Jayakody, P. (2008). Drivers and characteristics of wastewater agriculture in developing countries: results from a global assessment. Colombo, Sri Lanka: International Water Management Institute. Agriculture, Veterinary Science, Nutrition and Natural Resources, 3, 15.
Phuc, P.D., Konradsen, F., Phuong, P.T., Cam, P.D. and Dalsgaard, A. (2006). Practice of using human excreta as fertilizer and implications for health in Nghean Province, Vietnam. S E Asian J Trop Med Public Health, 37, 222- 229.
Asano, T., Burton, H., Tsuchihashi, R., Tchobanoglous, G. (2007). Water reuse: Issues, Technologies, and Applications. McGraw-Hill Professional, New York p1570.
WHO, (2006) Guidelines for the safe use of wastewater, excreta and greywater - Volume 2: Wastewater use in agriculture. In. Geneva, Switzerland: World Health Organisation.
Carr, R. and Strauss, M. (2001). Excreta-related infections and the role of sanitation in the control of transmission. In Water quality: Guidelines, standards and health, assessment of risk and risk management of water- related infectious disease. Edited by Fewtrell L, Bartram J. Lodon: International Water Association; 89-113.
Morgan, P. (2003). Experiments using urine and humus derived from ecological toilets as a source of nutrients for growing crops. In The 3rd World Water Forum; KYOTO, JAPAN.
Jensen PK, Phuc PD, Knudsen LG, Dalsgaard A, Konradsen F. (2008). Hygiene versus fertiliser: the use of human excreta in agriculture--a Vietnamese example. Int J Hyg Environ Health 211: 432-439.
Jensen PKM, Phuc PD, West LGK: (2010). How do we sell the hygiene message? withdollars, dong or excreta? Environ Health 9: 27.
Drechsel. P., Scott, C.A., Raschid-Sally, L., and Redwood, A. B. (2010). Wastewater irrigation and health - Assessing and mitigating risk in low- income countries. In. Sterling, VA, London: Earthscan.
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