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Anti-bacterial Activity of Tithonia diversifolia, Secamore afzelli and Jaundea pinnata Against Plasmid-bearing Multiple Antibiotics Resistant Bacteria from Different Water Sources in Akure, Nigeria
Advances in Bioscience and Bioengineering
Volume 7, Issue 2, June 2019, Pages: 22-26
Received: Jun. 6, 2019; Accepted: Jul. 10, 2019; Published: Jul. 26, 2019
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Alaofin Sefunmi, Department of Microbiology, Federal University of Technology, Akure, Nigeria
Onifade Anthony Kayode, Department of Microbiology, Federal University of Technology, Akure, Nigeria
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This study aims at assessing the antibacterial efficacies of some plant leaves. In this study, the antibacterial efficacies of Tithonia diversifolia Harm, Secamore afzelli Linn, and Jaundea pinnata Linn were assayed against plasmid-bearing multiple antibiotics resistant Gram-negative bacteria isolated from different water sources in Akure local government, Nigeria. The extracts of the plant leaves were prepared using cold water, hot water, Petroleum ether and Ethanolic solvents. The plant leaves of Tithonia diversifolia, Secamore afzelli and Jaundea pinnata were air-dried and pulverized using an electric blender which were soaked in 1 liter of solvent each for 72 hours after which it was sieved using muslin cloth and filtered using Whatman No 1 filter paper. Filtrates collected in beaker were concentrated in vacuousing rotary evaporator. The extracts were then reconstituted in tween 20 and sterilized with the aid of Millipore membrane filter. Agar well diffusion technique was done by using 1 ml aliquot of 18 hours broth culture that had been adjusted to the 0.5 McFarland standards which was dispensed into sterile Petri dishes and molten sterile Muller-Hinton agar was aseptically poured into the plates and incubated at 37°C for 24 hours. Clear zones around the wells were measured in millilitres. The minimum inhibitory concentration of extracts that showed antimicrobial activity were reconstituted by diluting 0.5 g of each in 10 ml of Tween 20 and then sterilized by passing through sterile Millipore membrane filter (0.45 µl). Different concentration of the extracts (50, 25, 12.5, 6.25, 3.125 mg/ml) was used. From this research work, it was observed that the highest plant extract yield was seen in the ethanolic extracts of Tithonia diversifolia, Secamore afzelli and cold water extracts of Jaundea pinnata having 17.8%, 8.6% and 15.7% respectively while the lowest yield was observed in petroleum ether extracts of Secamore afzelli. From the antibacterial analysis, it was observed that Hot water extract of J. pinnata inhibited Acinetobacter baumanni, Salmonella typhimurum while the cold water extracts of T. diversifolia inhibited Enterobacter aerogenes and Shigella dysenteriae.
Antibacterial Efficacies, Plasmid-bearing, Multiple Antibiotics Resistant Bacteria
To cite this article
Alaofin Sefunmi, Onifade Anthony Kayode, Anti-bacterial Activity of Tithonia diversifolia, Secamore afzelli and Jaundea pinnata Against Plasmid-bearing Multiple Antibiotics Resistant Bacteria from Different Water Sources in Akure, Nigeria, Advances in Bioscience and Bioengineering. Vol. 7, No. 2, 2019, pp. 22-26. doi: 10.11648/
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This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Gutierrez, R., Balladan, K. andPatacsil, M. (2015). The antibacterial property of Tithoniadiversifolia extract from Baguio-Benguetareas in the Philippines in response to exposure to vehicular traffic. Asian Journal of Microbiology, Biotechnology and Environmental sciences. 17 (1): 43-52.
Lindberg, D. (2008). The beginnings of western science: The European scientific tradition in philosophical, religious, and institutional context, prehistory to A. D. 1450. Second edition. Chicago: University of Chicago Press. Pp: 22.
Akinyemi, K. O., Oluwa, O. K., and Omomigbehin, E. O. (2006). Antimicrobial activity of crude extract of 3 medicinal plants used in south-west Nigeria folk medicine on some food-borne bacterial pathogens. African Journal of Traditional, Complementary and alternative medicine. 3 (4): 13-22.
Olanrewaju, R. A., Ale, E. M. and Akinwale, S. O.(2017). Qualitative and Quantitative Evaluation of the Phytochemical in dry, Wet and Oil Extracts of the leaf of Morinda lucida. Journal of Biology, Agriculture and Health Care. 7 (7): 22-25.
Ogundare, A. O. and Onifade, A. K. (2009). The antimicrobial activity of Morinda lucida leaf extract on Escherichia coli. Journal of Medicinal Plant Research, 3 (4): 319-323.
Cheesbrough, M. (2000). District Laboratory Practice in Tropical Countries, 2nd edition, Cambridge University Press, New York, Pp. 132-136.
Cheesbrough, M. (2010). District Laboratory Practice in Tropical Countries part two, Cambridge University press, New York, Pp. 70-71.
Kordali, S., Cakir, A. and Drum, M. (2003). “Antifungal activity of the leave of three Pistacies from Turkey” Fitoterapia, 74: 164-167.
Srinivasan, D., Perumalsamy L., Nathan, S. and Sures, T. (2001). “Antimicrobial activity of certain Indian medicinal plants used in folkloric medicine”. Journal of Ethnopharmacology, 94: 12-14.
Campos, A. R., Rao, V. S. and Printed A. G. (2002). “Investigation in the antirocaceptive activity of crude extracts from Croton caficara leaves in mice”. Fitoterpia, 73: 116-120.
Doughari, J., Pukuma, M. and De N. (2007). “Antibacterial effects of BalanitisaegyptiacaL. Drel and Moringa oleifera (Lam) on Salmonella typhi”. African Journal of Biotechnology, 6 (19): 2212-2215.
Oladunmoye M. K. (2005). “Comparative studies on the antimicrobial activities of leaf extracts from six Cassia species” PhD Thesis, the Federal University of Technology, Akure. Pp86-90.
Mailard, J. Y. (2002). Bacterial target sites for biocide action. Journal of Applied Microbiology. 9: 16-27.
Samah, H. Y., Abeer, E., Sayed, H. A and Hanaiya, A. E. (2014) Chemical analysis, Antibacterialactivity and genetic diversity assessment of some Egyptian Citrus spp. Cultivars. African Journal of Biotechnology, 13 (26): 2626 -2636.
Ogunfolakan O., Kolawole, O. S. and Olowe, A. O. 2010. In vitro antimicrobial activity of Tithonia diversifolia leaf extracts on bacterial isolates from wound infections from a Nigerian hospital. Research Journal of Medical Sciences. 4 (5): 305-308.
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