Comparative Isolation of Escherichia coli 0157:H7 from Diarrhoeic and Non-Diarrhoeic Children in Selected Communities in Cross River State, Nigeria
Advances in Bioscience and Bioengineering
Volume 6, Issue 2, June 2018, Pages: 23-29
Received: Jun. 10, 2018; Accepted: Jul. 4, 2018; Published: Aug. 15, 2018
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Authors
Nfongeh Joseph Fuh, Department of Microbiology, Faculty of Science, Federal University, Lafia, Nigeria
Owoseni Mojisola Christiana, Department of Microbiology, Faculty of Science, Federal University, Lafia, Nigeria
Upla Peter Uteh, Department of Microbiology, Faculty of Science, Federal University, Lafia, Nigeria
Odonye Dauda Dantani, Department of Microbiology, Faculty of Science, Federal University, Lafia, Nigeria
Akharenegbe Pedro, Department of Microbiology, Faculty of Science, Federal University, Lafia, Nigeria
Fadayomi Victor Kolawole, Department of Microbiology, Faculty of Science, Federal University, Lafia, Nigeria
Uchenwa Mercy Ogechi, Department of Microbiology, Faculty of Biological Sciences, University of Calabar, Calabar, Nigeria
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Abstract
Escherichia coli O157:H7 has been considered an emerging foodborne pathogen causing severe diarrheal disease globally especially among children under the age of five years in Sub-Saharan Africa. This study was aimed at conducting a comparative study on the rate of isolation of Escherichia coli O157:H7 from diarrhoeic and non- diarrhoeic children in selected communities in Cross River State, Nigeria. Stool samples were collected from children under the age of five yrs and the pathogen isolated and identified using standard microbiological and biochemical procedures. Serological analysis to detect E. coli 0157:H7 serotype was carried out using Enzyme-linked immunosorbent assay (ELISA) and anti-rabbit H7 latex serum agglutination techniques. Out of 367 diarrhoeic children sampled, 70 (19.07%) were positive for E. coli 0157:H7 and the prevalence differed significantly (p<0.05) with the control. The prevalence of E. coli 0157:H7 between diarrhoeal (19.07%) and non-diarrhoeal (1.39%) cases also differed significantly (p<0.05) among the sampling areas. Diarrhoeric children below the age of one year had significantly highest prevalence of 26.83% at p<0.05 though no significant relationship between the sex of the children and the rate of infection with the organism was observed. Children diarrhoaeic stool therefore serves as a major vehicle in the domestic transmission of Escherichia coli O157:H7. Improved personal hygiene and environmental sanitation among parents and care givers can reduce the spread of diarrheal disease caused by this pathogen amongst children under the age of five years.
Keywords
Escherichia coli O157:H7, Childhood Diarrhoea, Nigeria
To cite this article
Nfongeh Joseph Fuh, Owoseni Mojisola Christiana, Upla Peter Uteh, Odonye Dauda Dantani, Akharenegbe Pedro, Fadayomi Victor Kolawole, Uchenwa Mercy Ogechi, Comparative Isolation of Escherichia coli 0157:H7 from Diarrhoeic and Non-Diarrhoeic Children in Selected Communities in Cross River State, Nigeria, Advances in Bioscience and Bioengineering. Vol. 6, No. 2, 2018, pp. 23-29. doi: 10.11648/j.abb.20180602.12
Copyright
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
References
[1]
Azar, D. K.; Sohella, K; Ahmed F. S; Ali A. and Ahmad S. (2016). Prevalence of Escherichia coli 0157:H7 in children with bloody diarrhea. Referring to Abuzar Teaching Hospital, Ahvaz, Iran Journal of Clinical and Diagnostic Research 10 (1):13-15.
[2]
Kosek. M., Bern, C. and Guerrant, R. L. (2003). The global burden of diarrhoeal disease, as estimated from studies published between 1992 and 2000. Bull WHO. 81:197–204.
[3]
World Health Organization (2018) Escherichia coli outbreaks. W. H. O. Publication Geneva Switzerland
[4]
Nfongeh J. F; Epoke, J; Antai, E. E; Ikpeme, E. M; Etim, L. B; Akeh, M. and Ekpiken, S. E. (2014). The Effects of Escherichia coli 0157:H7 lipopolysaccharide (LPS) from human, cattle and poultry isolates on haematological parameters of neonatal albino rats. European Journal of Experimental Biology 4 (1):538-542
[5]
Ochoa, T. J., Salazar-Lindo, E. and Cleary, T. G. (2004). Management of children with infection-associated persistent diarrhea. Semin Paediatr Infect Dis. 15 (4):229–36.
[6]
Sousa, C. P. (2006). Escherichia coli as a specialized bacterial pathogen. Revista De Biologia E Ciências Da Terra. 6 (2):341-349.
[7]
Luo, Y., Cui, S., Li, J., Yang, J., Lin, L., Hu, C., Jin, S., Ye, L., Zhao, Q. and Ma, Y. (2011). “Characterization of Escherichia coli isolates from healthy food handlers in hospital”, Microbial Drug Resistance, 17. 443-448.
[8]
Stewardson, A. J., Renzi, G., Maury, N., Vaudaux, C., Brassier, C., Fritsch, E., Pittet, D., Heck, M., van der Zwaluw, K. and Reuland, E. A. (2014). “Extended-Spectrum β- Lactamase-Producing Enterobacteriaceae in Hospital Food: A Risk Assessment”. Infection Control & Hospital Epidemiology, 35. 375-383.
[9]
Havelaar, A. H., Kirk, M. D., Torgerson, P. R., Gibb, H. J., Hald, T., Lake, R. J., Praet, N., Bellinger, D. C., De Silva, N. R. and Gargouri, N. (2015). “World Health Organization Global estimates and regional comparisons of the burden of food borne disease in 2010”, PLoS Med, 12. e1001923.
[10]
Greig, J, D., Todd, E. C. D., Bartleson, C. and Michaels, B. (2010). "Infective Doses and Pathen Carriage". USDA (2010) Food Safety Education Conference. pp. 19–20.
[11]
Gally, D. L. and Stevens, M. P. (2017). "Microbe Profile: Escherichia coli O157:H7 - notorious relative of the microbiologist's workhorse". Microbiology. 163 (1): 1–3.
[12]
Okeke, I. N. (2009). Regional Review Diarrheagenic Escherichia coli in -Saharan Africa: Status, uncertainties and necessities. J Infect Dev Ctries. 3: 817-842.
[13]
National Bureau of Statistics. (2012). Annual Abstract of Statistics, 2012. pp 23.
[14]
Presteri, E., Zwick, R. H., Reichmann, S., Aichelburg, A., Winkler, S. & Kremsner, P. G. (2003). Frequency and virulence properties of diarrhoeagenic E. coli in children with diarrhoea in Gabon. American Journal of Tropical Medicine and Hygiene.69:406-410
[15]
Milley, D. G, & Sekia, L. H., (1993). An Enzyme-linked Immunosorbent Assay-based isolation procedure for verotoxigenic E. coli. Applied and Environmental Microbiology. 59 (12):4223-4229
[16]
Nfongeh, J. F., Udo, S. M. and Lennox, J. A. (2005). Impact of water availability on diarrhoeal morbidity in two contrasting communities in Cross River State, Southern Nigeria. Global Journal of Pure and Applied Sciences. 11 (3):363-366
[17]
Egbule, O. S., Owhe-Ureghe, U. B. and Odih, E. E. (2016). Occurrence of Multidrug Resistance among E. coli O157:H7 Isolated from Stool Samples Obtained from Hospitalized Children. J Prob Health 4:150.
[18]
Sani, A., Onaolapo, J. A., Ibrahim, Y. K. E., Idris, H. W., Igwe, J. C. and Nworie, A. (2015). Prevalence of Escherichia coli Pathotypes among Children with Diarrhoea in Zaria, Nigeria. British Journal of Medicine & Medical Research. 7 (1): 17-24.
[19]
Itelima, J. U., Agina, S. E., Ogbonna, A. I. and Nwaukwu, I. A. (2014). The Occurrence of Escherichia Coli Serotype O157: H7 among Humans in Some Parts of Plateau State, Nigeria. Developmental Microbiology and Molecular Biology. 5 (1): 9-20.
[20]
Isibor, J. O., Afe, O. E., Regina, E. O. and Philip, O. O. (2013). Escherichia coli O157:H7-Prevalence and Risk Factors of Infection in Edo State, Nigeria American Journal of Research Communication. 3 (1): 35-49.
[21]
Fard, A. H. M., Bokaeian M. and Qureishi, M. E. (2008). Frequency of Escherichia coli O157:H7 in children with diarrhoea in Zahedan, Islamic Republic of Iran. La Revue de Santé de la Méditerranée orientale. (14) 5: 1022-1027.
[22]
Newell, D. G. and Ragione, R. M. (2018) Enterhaemorrhagic and other Shiga toxin-producing Escherichia coli (STEC): where are we now regarding diagnostic and control strategies. Transboundary and Emerging Diseases 65 Suppl 1 (57)
[23]
Chapman, P. A., Siddons, C. A., Cerdan-Malo, A. T. and Harkin, NM. A. (1997). A 1-year study of Escherichia coli 0157 in cattle, sheep, pigs and poultry. Epidemiology and Infection. 119:245-250.
[24]
Majowicwz, S. E; Scallan E. and Jones-Bitton A. (2014). Global incidence of Shiga toxin-producing Escherichia coli infections and deaths: a systematic review and knowledge synthesis. Foodborne Pathogens and Diseases 11 (6):447-455
[25]
Su, C. & Brandt, L. J. (1995). Escherichia coli 0157:H7 infection in humans. Annals of Internal Medicine 123:698-714
[26]
Gomes, T. A., Elias, W. P., Scaletsky, I. C., Guth, B. E., Rodrigues, J. F., Piazza, R. M., Ferreira, L. C. and Martinez, M. B. (2016). Diarrheagenic Escherichia Coli. Braz J Microbiol. 47 (Suppl 1):3–30
[27]
Gould, L. H., Walsh, K. A., Vieira, A. R., Herman, K., Williams, I. T., Hall, A. J. and Cole, D. (2013). ''Surveillance for food borne disease outbreaks-United States, 1998-2008'', MMWR Surveill Summ, 62. 1-34.
[28]
Abdulaziz, H. O; Aminu, M. and Machido A (2016). Isolation and characterization of Escherichia coli 0157:H7 in Human stool samples from parts of Kaduna Metropolis, Nigeria. American Journal of Food Science and Technology 4 (5):125-128
[29]
Reuben, C. R. and Gyar S. D. (2015). Isolation and Antibiogram of Shiga toxin-producing Escherichia coli 0157:H7 from Diarrhoeic HIV/AIDS patients in Lafia Central Nigeria. International Research Journal of Microbiology 6 (2):20-26.
[30]
Moyo, S. J., Gro, N., Matee, M. I., Kitundu, J., Myrmel, H., Mylvaganam, H., Maselle, S. Y. and Langeland, N. (2011). Age specific aetiological agents of diarrhoea in hospitalized children aged less than five years in Dar es Salaam, Tanzania. BMC Pediatr.11:19.
[31]
Nataro, J. P. and Kaper, J. B. (1998). Diarrheagenic Escherichia coli. Clin Microbiol Rev. 11:142–201.
[32]
Patzi-Vargas, S., Zaidi, M. B., Perez-Martinez, I., Leon-Cen, M., Michel-Ayala, A., Chaussabel, D. and Estrada-Garcia, T. (2015). Diarrheagenic Escherichia Coli carrying supplementary virulence genes are an important cause of moderate to severe diarrhoeal disease in Mexico. PLoS Negl Trop Dis. 9 (3): e0003510.
[33]
Kosek. M., Bern, C. and Guerrant, R. L. (2003). The global burden of diarrhoeal disease, as estimated from studies published between 1992 and 2000. Bull WHO. 81:197–204.
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