Design of Portable Time-Resolved Fluorometer
Advances in Bioscience and Bioengineering
Volume 4, Issue 6, December 2016, Pages: 79-84
Received: Dec. 2, 2016; Published: Dec. 5, 2016
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Chongde Zi, Color & Image Vision Lab, Yunnan Normal Univ, Kunming, China
Junsheng Shi, Color & Image Vision Lab, Yunnan Normal Univ, Kunming, China
Yonghang Tai, Color & Image Vision Lab, Yunnan Normal Univ, Kunming, China
Huan Yang, Color & Image Vision Lab, Yunnan Normal Univ, Kunming, China
Xicai Li, Color & Image Vision Lab, Yunnan Normal Univ, Kunming, China
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A miniaturization time-resolved fluorescence analyzer was designed based on immunofluorescence and embedded technology, this instrument can be utilized to detect the immunochromatographic strip to achieve quantitative inspection and analysis of analytes in human blood or body fluid. By mechanical scanning means point-by-point assay, using UV-LED irradiation the fluorescence labeled immunocomplex on the immunochromatographic strip, and the fluorescence was emitted after being excited. Fluorescent light passes through narrow-band optical filters and converted to electrical signal by Silicon photodiode (Si PIN). After amplification and analog-digital conversion, the signal is sent to the micro-controller STM32F103 for processing. The fluorescence intensity distribution of the reagent strip was obtained after the scan detection finished, and the concentration of the substance can be calculated based on the known standard curve. After testing, the instrument has a wide dynamic range of measurement. It not only has a reliable repeatability (CV<0.2%) performance in the detection of concentration larger than 100µg/mL, but also hasdemonstrate stable validate features (CV=2.6%) when the concentration only at 1µg/mL. It has commendably application prospects in point-of-care test detection (POCT).
Time-Resolved, Fluorescence Immunoassay, TRFIA, Quantitative Detection, Si PIN, POCT
To cite this article
Chongde Zi, Junsheng Shi, Yonghang Tai, Huan Yang, Xicai Li, Design of Portable Time-Resolved Fluorometer, Advances in Bioscience and Bioengineering. Vol. 4, No. 6, 2016, pp. 79-84. doi: 10.11648/
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