Design of Portable Time-Resolved Fluorometer
Advances in Bioscience and Bioengineering
Volume 4, Issue 6, December 2016, Pages: 79-84
Received: Dec. 2, 2016; Published: Dec. 5, 2016
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Authors
Chongde Zi, Color & Image Vision Lab, Yunnan Normal Univ, Kunming, China
Junsheng Shi, Color & Image Vision Lab, Yunnan Normal Univ, Kunming, China
Yonghang Tai, Color & Image Vision Lab, Yunnan Normal Univ, Kunming, China
Huan Yang, Color & Image Vision Lab, Yunnan Normal Univ, Kunming, China
Xicai Li, Color & Image Vision Lab, Yunnan Normal Univ, Kunming, China
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Abstract
A miniaturization time-resolved fluorescence analyzer was designed based on immunofluorescence and embedded technology, this instrument can be utilized to detect the immunochromatographic strip to achieve quantitative inspection and analysis of analytes in human blood or body fluid. By mechanical scanning means point-by-point assay, using UV-LED irradiation the fluorescence labeled immunocomplex on the immunochromatographic strip, and the fluorescence was emitted after being excited. Fluorescent light passes through narrow-band optical filters and converted to electrical signal by Silicon photodiode (Si PIN). After amplification and analog-digital conversion, the signal is sent to the micro-controller STM32F103 for processing. The fluorescence intensity distribution of the reagent strip was obtained after the scan detection finished, and the concentration of the substance can be calculated based on the known standard curve. After testing, the instrument has a wide dynamic range of measurement. It not only has a reliable repeatability (CV<0.2%) performance in the detection of concentration larger than 100µg/mL, but also hasdemonstrate stable validate features (CV=2.6%) when the concentration only at 1µg/mL. It has commendably application prospects in point-of-care test detection (POCT).
Keywords
Time-Resolved, Fluorescence Immunoassay, TRFIA, Quantitative Detection, Si PIN, POCT
To cite this article
Chongde Zi, Junsheng Shi, Yonghang Tai, Huan Yang, Xicai Li, Design of Portable Time-Resolved Fluorometer, Advances in Bioscience and Bioengineering. Vol. 4, No. 6, 2016, pp. 79-84. doi: 10.11648/j.abb.20160406.13
References
[1]
J. Karamehic, D. Subasic, F. Gavrankapetanovic, et al, "The incidence of antinuclear antibodies (ANA) detected by indirect immunofluorescence assay (IFA) method,” Medicinski Arhiv, vol. 61, 2007, pp. 16-19.
[2]
Zhancheng Gao, “Application of the indirect immunofluorescence assay against specific antibodies in the diagnosis of infectious respiratory diseases,” Chinese Journal of Laboratory Medicine, vol. 35, 2012, pp. 697-700.
[3]
H. Déchaud, R. Bador, F. Claustrat, et al, “New approach to competitive lanthanide immunoassay: time-resolved fluoroimmunoassay of progesterone with labeled analyte,” Clinical Chemistry, vol. 34, 1988, pp. 501-504.
[4]
A. A. Oraevsky, S. L. Jacques, and F. K. Tittel, “Measurement of tissue optical properties by time-resolved detection of laser-induced transient stress,” Applied Optics, vol. 36, 1997, pp. 402-415.
[5]
Jian Shen, Deqiu Lin, and Jie Xu, “Present situation and progress of time-resolved fluoroimmunoassay,” Chinse Bulletin Life Sciences, vol.16, 2004, pp. 55-59.
[6]
Xuecheng Wu, Lin He, and Keyuan Zhou, “The Research and Clinical Application of Time- resolved Fluoroimmunoassay,” Medical Recapitulate, vol 12, 2006, pp. 434-436.
[7]
Q. Liao, X. A. L. Liu, and H. Guo, “The discussion of Time-resolved immunofluorescence method for determination of syphius antibody,” Chinese Journal of Laboratory Diagnosis, vol. 18, 2014, pp. 447-450.
[8]
Xiaoyu Fu, Feiyuan Wu, and Gang Chen, et al, “Feasibility analysis of quantitative detection on serum HBeAg/HBeAb by time-resolved immunofluorescence assay,” Journal of Central South University (Medical Science), vol. 41, 2016, pp. 852-855.
[9]
Jianfeng Hang, Ying Song Wu, and Ming Li, "New Progress in the Research on Time-resolved Fluoroimmunoassay." Journal of Tropical Medicine, vol. 4, 2004, pp. 340-343.
[10]
H. Siitari, I. Hemmilä, E. Soini, T. Lövgren, et al, “Detection of hepatitis B surface antigen using time-resolved fluoroimmunoassay,” Nature, vol. 301, 1983, pp. 258-260.
[11]
J. R. Lakowicz, “Emerging Biomedical Applications of Time-Resolved Fluorescence Spectroscopy,” Topics in Fluorescence Spectroscopy. Springer US, vol. 2136, 2002, pp. 1-19.
[12]
Poras, Herve, T. Ouimet, and M. C. Fournie-Zaluski, “substrates the fluorescence of which is suppressed, preparation thereof, and use thereof for identifying, detecting, and assaying legionella pneumophilia,” US20130203097. 2013.
[13]
P. B. Luppa, C. Müller, A. Schlichtiger, et al, “Point-of-care testing (POCT), pp. Current techniques and future perspectives,” Trac Trends in Analytical Chemistry, vol. 30, 2011, pp. 887-898.
[14]
R. H. Shyu, H. F. Shyu, H. W. Liu, “Colloidal gold-based immunochromatographic assay for detection of ricin,” Toxicon Official Journal of the International Society on Toxinology, vol.40, 2002, pp. 255-258.
[15]
B. B. Dzantiev, N. A. Byzova, A. E. Urusov, et al, "Immunochromatographic methods in food analysis,” Trac Trends in Analytical Chemistry, vol.55, 2014, pp. 81-93.
[16]
Yan Zhang, Guangyu Zeng, Zhigang Hong, "Research on the Silicon PIN Diode Detecting System,” Computer Development & Applications, vol.20, 2007, pp. 7-8.
[17]
C. E. Brown, Coefficient of Variation. Springer US, 2006, pp. 6.
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