Diversity of Arbuscular Mycorrhizal Fungi of Different Plant Species Grown in Three Land Use Types in Wensho and Shebidino Districts of Sidama in Southern Ethiopia
Advances in Bioscience and Bioengineering
Volume 4, Issue 4, August 2016, Pages: 25-34
Received: Jun. 13, 2016;
Accepted: Jul. 1, 2016;
Published: Jul. 21, 2016
Views 4428 Downloads 277
Beyene Dobo, Department of Natural Resources Management and Environmental Sciences, Haramaya University, Haramaya, Ethiopia
Fassil Asefa, Department of Microbial, Cellular and Molecular Biology, Addis Ababa University, Addis Ababa, Ethiopia
Zebene Asfaw, Wondo Genet College of Forestry and Natural Resources, Hawassa University, Hawassa, Ethiopia
Diversity of arbuscular mycorrhizal fungi (AMF) of culturally protected forest, agroforestry practices, and mono-cropping lands has been investigated in Wensho and Shebedino districts of Sidama Zone in Southern Ethiopia. Rhizosphere soil and root samples of plant components from each land use type were analyzed for spore density, diversity and AM-root colonization. Except some non-mycorrhizal plants, all plants surveyed in the three land-use types showed AMF colonization ranging from 50 to 91%. A total of 29 AMF morphospecies, belonging to nine genera (Acaulospora, Glomus, Claroideoglomus, Funneliformis, Pacispora, Septoglomus, Rhizophugus, Scutellospora and Gigaspora), were identified in the rhizospheres of selected plants in the three land uses. Spores of four genera Rhizophugus, Glomus, Funneliformis, and Acaulospora had higher spore production, accounting for 36.22%, 21.20%, 19.39%, 17.54% and 11.74% of the total number of spores respectively. One-way analysis of variance (ANOVA) showed that spore density and root colonization of different AM structures varied greatly among plant species both within and between different land-use types. Spore density was higher in culturally protected forest and AM colonization was higher in the agroforestry. The lowest number of spores and the lowest percentage of root colonization were recorded in cropland. When land use types were considered separately or together no significant correlation between spore densities and AM colonization was observed. The result of the study indicates that mono-cropping reduces spore density and AM colonization in comparison with the culturally protected forest and the agroforestry.
Diversity of Arbuscular Mycorrhizal Fungi of Different Plant Species Grown in Three Land Use Types in Wensho and Shebidino Districts of Sidama in Southern Ethiopia, Advances in Bioscience and Bioengineering.
Vol. 4, No. 4,
2016, pp. 25-34.
Allen, M. F., Helm, D. T., Trappe, J. M., Molina, R. and Rincón, E. (1995). Patterns and regulations of mycorrhizal plant and fungal diversity. Plant Soils, 170: 47-62.
Amerian, M. R. and Stewart, W. S. (2001). Effect of 2 species of arbuscular mycorrhizal fungi on growth assimilation and leaf water relations in maize (Zea mays). Aspects of Applied Biology, 63: 1-6.
Azcón-Aguilar, C. and Barea, J. M. (1996). Arbuscular mycorrhizas and biological control of soil-borne plant pathogens-an overview of the mechanisms involved. Mycorrhiza, 6: 457-464.
Brundrett, M., Melville, L. and Peterson, L. (1994). Practical Methods in Mycorrhiza Research. Mycologue Publications, University of Guelph, Guelph, Ontario, Canada.
Carrenho, R., Trufem, S. F. B., Bononi, V. L. R. and Silva, E. S. (2007). The effect of different soil properties on arbuscular mycorrhizal colonization of peanuts, sorghum and maize. Acta. Bot. Bras., 21: 23-730.
Catford, J., Staehelin, C., Lerat, S., Piché, Y. and Vierheilig, H. (2000). Suppression of arbuscular mycorrhizal colonization and nodulation in split-root systems of alfalfa after pre-inoculation and treatment with Nod factors. Journal of Experimental Botany, 54: 481-1487.
Chen, K., Liu, W., Guo, S., Liu, R. and Li, M. (2012). Diversity of arbuscular mycorrhizal fungi in continuous cropping soils used for pepper production. African Journal of Microbiology Research, 6: 469-2474.
Douds, D. D. and Millner, P. (1999). Biodiversity of arbuscular mycorrhizal fungi in agroecosystems. Agriculture, Ecosystem and Environment, 74: 7-93.
Duponnois, R., Plenchette, C., Thioulouse, J. and Cadet, P. (2001). The mycorrhizal soil infectivity and arbuscular mycorrhizal fungal spore communities in soils of different and aged fallows in Senegal. Applied Soil Ecology, 17, 239-251.
EFAP. 1993. Ethiopian forestry action program: the challenge for development (Vol. I). Ministry of Natural Resources Development and Environmental Protection, Addis Ababa, Ethiopia.
Emmanuel, B., Fagbola, O., Abaidoo, R., Osonubi, O. and Oyetunji, O. (2010). Abundance and distribution of arbuscular mycorrhizal fungi species in long-term soil fertility management systems in Northern Nigeria. Journal of Plant Nutrition, 33: 264-1275.
Enkhtuya, B., Rydlová, J. and Vosátka, M. (2000). Effectiveness of indigenous and non-indigenous isolates of arbuscular mycorrhizal fungi in soils from degraded ecosystems and man-made habitats. Applied Soil Ecology, 14, 201-211.
Jefwa, J. M., Mung’atu, J., Okoth, P., Muya, E., Roimen, H. and Njuguini, S. (2009). Influence of land use types on occurrence of arbuscular mycorrhizal fungi in the high altitude regions of Mt. Kenya. Tropical and Subtropical Agroecosystems, 11: 77- 290
Li, L. F., Li T. and Zhao, Z. W. (2007). Differences of arbuscular mycorrhizal fungal diversity and community between a cultivated land, an old field, and a never-cultivated field in a hot and arid ecosystem of southwest China. Mycorrhiza 17: 55-665.
Mason, P. A., Musoko, M. O. and Last, F. T. (1992). Short-term changes in vesicular-arbuscular mycorrhizal spore populations in Terminalia plantations in Cameroon. In: Read, D. J., Lews, D. H., Fitter, AH., Alexander, I. J (eds.). Mycorrhizas in ecosystems. Wallingford: CAB International. Pp. 261-267.
Mathimaran, N., Ruh, R., Jama, B., Verchont, L. and Frossard. (2007). Impact of agricultural management on arbuscular mycorhizal fungal communities in Kenyan ferrasol. Agriculture Ecosystems and Environment, 119: 2-32.
McGonigle, T. P., Evans, D. G. and Miller, R. M. (1990). Effect of degree of soil disturbance on mycorrhizal colonization and phosphorus absorption by maize in growth chamber and field experiments. New Phytologist, 116: 629-636.
Muleta, D., Assefa, F., Nemomissa, S. and Granhall, U. (2007). Composition of coffee shade tree species and density of indigenous arbuscular mycorrhizal fungi (AMF) spores in Bonga natural coffee forest, southwestern Ethiopia. Forest Ecology and Management, 241: 45-154.
Muthukumar, T., Sha, L. Q., Yang, X. D., Cao, M., Tang, J. W. and Zheng, Z. (2003b). Mycorrhiza of plants in different vegetation types in tropical ecosystems of Xishuangbanna, southwest China. Mycorrhiza, 13, 289-297.
Phillips, J. M. and Hayman, D. S. (1970). Improved procedure for clearing roots and staining parasitic and vesicular arbuscular mycorrhizal fungi for rapid assessment of infection. Trans Br Mycol Soc., 55: 58-161
Richter, B. S., Tiller, R. L. and Stutz, J. C. (2002). Assessment of arbuscular mycorrhizal fungal propagules and colonization from abandoned agricultural fields and semi-arid grasslands in riparian flood-plains. Applied Soil Ecology, 20: 227-238.
Schenck, N. C. and Pérez, Y. (1990). Manual for the Identification of VA Mycorrhizal Fungi. Synergistic-Publications, Gainesville, Florida.
Smith, S. E. and Read, J. D. (2008). Mycorrhizal symbiosis, 2nd ed. Academic Press Ltd., London, England.
Steinberg, P. D. and Rillig, M. C. (2003). Differential decomposition of arbuscular mycorrhiza fungal hyphae and glomalin. Soil Biology and Biochemistry, 35: 191-194.
Stürmer, S. L. Siqueira, J. O. (2011). Species richness and spore abundance of arbuscular mycorrhizal fungi across distinct land uses in Western Brazilian Amazon. Mycorrhiza, 21: 55-267.
Tchabi, A, Coyne, D., Hountondji, F., Lawouin, L., Wiemken, A. and Oehl, F. (2008). Arbuscular mycorrhizal fungal communities in sub-Saharan Savannas of Benin, West Africa, as affected by agricultural land use intensity and ecological zone. Mycorrhiza, 18: 81-195.
Tilal, S. A., Maria, R. F., Abdel, G. B. and Fritz, O. (2013). Species composition and diversity of arbuscular mycorrhizal fungi in White Nile state, Central Sudan, Archives of Agronomy and Soil Science.
Trappe, J. (1987). Phylogenetic and ecologic aspects of mycotrophy in the angiosperms from an evolutionary standpoint In: Safir GR, ed. Ecophysiology of VA mycorrhizal plants. Boca Raton, Florida, USA: CRC Press, pp. 5-25.
Uhlmann, E., Görke, C., Petersen, A. and Oberwinkler, F. (2004). Arbuscular mycorrhizae from semiarid regions of Namibia. Canadian Journal of Botany 82: 45-653.
Van der Heijden, M. G., Klironomos, J. N., Ursic, M., Moutoglis, P., Streitwolf-Enge, l R., Boller, T., Wiemken, A. and Sanders, I. R. (1999). Sampling effect’, a problem in biodiversity manipulation? A reply to David A. Wardle. Oikos. 87: 408-410.
Zhao, Z., Xia, Y., Qin, X., Li, X., Cheng, L., Sha, T. and Wang G. (2001). Arbuscular Mycorrhizal status of plants and the spore density of arbuscular Mycorrhizal fungi in the tropical rain forest of Xishuangbanna, southwest China. Mycorrhiza, 11: 159-162.
Zerihun Belay, Vestberg, Z. M, Fassil Assefa. (2014). Diversity and abundance of arbuscular mycorrhizal fungi associated with acacia trees from different land use systems in Ethiopia. Africa Journal of Microbiology Research, 7 (48): 503-5515.