LPS Treatment and Exposure to PEMF induce Cell Death and Change in Secretory Activity of HMVEC-Bd with MM6 Cocultutre
Advances in Bioscience and Bioengineering
Volume 2, Issue 3, June 2014, Pages: 30-36
Received: Aug. 27, 2014;
Accepted: Sep. 11, 2014;
Published: Sep. 30, 2014
Views 3028 Downloads 164
Kaszuba-Zwoińska Jolanta, Department of Pathophysiology, Medical College, Jagiellonian University, Cracow, Poland
Chorobik Paulina, Department of Immunology, Medical College, Jagiellonian University, Cracow, Poland
Nowak Bernadeta, Department of Immunology, Medical College, Jagiellonian University, Cracow, Poland
Ziomber Agata, Department of Pathophysiology, Medical College, Jagiellonian University, Cracow, Poland
Juszczak Kajetan, Department of Pathophysiology, Medical College, Jagiellonian University, Cracow, Poland
Zaraska Wiesław, Institute of Electron Technology, Cracow, Poland
Thor Piotr, Department of Pathophysiology, Medical College, Jagiellonian University, Cracow, Poland
Objective: Present studies are aimed to elucidate that pulsed electromagnetic field (PEMF) influences cell death parameters and cellular interactions in coculture model in response to inflammatory stimulus like E.coli endotoxin. Methods: We hypothesized that PEMF exposure will affect cell death rate in the experimental coculture model, composed of the human bladder microvascular endothelial cell line (HMEVEC-Bd) and MonoMac6 (MM6) cells previously activated with LPS, and exposed to PEMF (7Hz, 30mT) for three times with 24h intervals. Following the last electromagnetic exposure, we measured viability of cocultured and cultured cells by annexin V (AnV) - propidium iodide (PI) flow cytometry staining procedure to evaluate cell death parameters. The level of proinflammatory cytokine, cell adhesion molecules and vascular endothelial growth factor (IL-8, ICAM-1 and VEGF-A) was estimated by ELISA method in coculture and cell culture collected supernatants. Results: PEMF exposure of HMVEC-Bd and MM6 coculture caused decrease of measured cell death parameters (early and late apoptosis as well as necrosis) and diminished production of some inflammatory agents released in response to LPS activation, comparing to not stimulated with PEMF controls. Conclussion: Obtained results confirmed our hypothesis and showed out that PEMF exposure of HMVEC-Bd & MM6 coculture previously activated with LPS exerted an anti-inflammatory effect.
LPS Treatment and Exposure to PEMF induce Cell Death and Change in Secretory Activity of HMVEC-Bd with MM6 Cocultutre, Advances in Bioscience and Bioengineering.
Vol. 2, No. 3,
2014, pp. 30-36.
Birder L, Apodaca G, Groat W, Kanai A. Adrenergic- and capsaicin-evoked nitric oxide release from urothelium and afferent nerves in urinary bladder. American Journal of Physiology 275: F226–F229, 1998.
Butcher E. Leukocyte-Endothelial cell recognition: three (or more) steps to specificity and diversity. Cell 67: 1033-1036, 1991.
Carlos T, Harlan J. Leukocyte-Endothelial adhesion molecules. Blood 84: 2068-2101, 1994.
Delle Monache S, Angelucci A, Sanità P, Iorio R, Bennato F, Mancini F, Gualtieri G, Colonna RC. Inhibition of angiogenesis mediated by extremely low-frequency magnetic fields (ELF-MFs). PLoS One 8(11): e79309, 2013.
Dupont M, Spitsbergen J, Kim K, Tuttle J, Steers. Histological and neurotrophic changes triggered by varying models of bladder inflammation. Journal of Urology 166: 1111–1118, 2001.
Ferguson D, Kennedy I, Burton T. ATP is released from rabbit urinary bladder epithelial cells by hydrostatic pressure changes – a possible sensory mechanism? Journal of Physiology 505: 503–511, 1997.
Haraoka M, Hang L, Frendeus B, Godaly G, Burdick M, Strieter R, Svanborg C. Neutrophil recruitment and resistance to urinary tract infection. J Infect Dis 180: 1220–1229, 1999.
Hooton TM, Stamm WE. Diagnosis and treatment of uncomplicated urinary tract infection. Infect Dis Clin North Am 11: 551–581, 1997.
Hopper RA, VerHalen JP, Tepper O, Mehrara BJ, Detch R, Chang EI, Baharestani S, Simon BJ, Gurtner GC. Osteoblasts stimulated with pulsed electromagnetic fields increase HUVEC proliferation via a VEGF-A independent mechanism. Bioelectromagnetics 30(3): 189-97, 2009.
Horsley H, Malone-Lee J, Holland D, Tuz M, Hibbert A, Kelsey M, Kupelian A, Rohn JL. Enterococcus faecalis subverts and invades the host urothelium in patients with chronic urinary tract infection. PLoS One 8(12): e83637, 2013.
Juszczak K, Kaszuba-Zwoinska J, Thor PJ. Pulsating electromagnetic field stimulation of urothelial cells induces apoptosis and diminishes necrosis: new insight to magnetic therapy in urology. J Physiol Pharmacol 63(4): 397-401, 2012.
Kanasaki K, Yu W, von Bodungen M, Larigakis JD, Kanasaki M, Ayala de la Pena F, Kalluri R, Hill WG. Loss of β1-integrin from urothelium results in overactive bladder and incontinence in mice: a mechanosensory rather than structural phenotype. FASEB J 27(5): 1950-61, 2013.
Kaszuba-Zwoinska J, Chorobik P, Juszczak K, Zaraska W, Thor PJ. Pulsed electromagnetic field affects intrinsic and endoplasmatic reticulum apoptosis induction pathways in MonoMac6 cell line culture. J Physiol Pharmacol 63(5): 537-45, 2012.
Kaszuba-Zwoińska J, Ciećko-Michalska I, Madroszkiewicz D, Mach T, Słodowska-Hajduk Z, Rokita E, Zaraska W, Thor P. Magnetic field anti-inflammatory effects in Crohn's disease depends upon viability and cytokine profile of the immune competent cells. J Physiol Pharmacol 59(1): 177-87, 2008.
Kaszuba-Zwoińska J, Zdziłowska E, Chorobik P, Słodowska-Hajduk Z, Juszczak K,Zaraska W, Thor P. Pulsing Electromagnetic Field and Death of Proliferating Peripheral Blood Mononuclear Cells from Patients with Acute Myelogenic Leukemia. Adv Clin Exp Med 20(6): 721–727, 2011.
Kaszuba-Zwoinska J, Wojcik K, Bereta M, Ziomber A, Pierzchalski P, Rokita E, Marcinkiewicz J, Zaraska W, Thor P. Pulsating electromagnetic field stimulation prevents cell death of puromycin treated U937 cell line. J Physiol Pharmacol 61(2): 201-5, 2010.
Keresteci AG, Leers WD. Indwelling catheter infection. Can Med Assoc J 109: 711–713, 1973.
Kowalewska PM, Burrows LL, Fox-Robichaud AE. Intravital microscopy of the murine urinary bladder microcirculation. Microcirculation 18(8): 613-22, 2011.
Lambert PA. Mechanisms of antibioticresistance in Pseudomonas aeruginosa. J R Soc Med 95(Suppl. 41): 22–26, 2002.
Niemelä J, Henttinen T, Yegutkin GG, Airas L, Kujari AM, Rajala P, Jalkanen S. IFN-alpha induced adenosine production on the endothelium: a mechanism mediated by CD73 (ecto-5'-nucleotidase) up-regulation. J Immunol 172(3): 1646-53, 2004.
Ochodnicky P, Cruz C, Yoshimura N, Michel MC. Nerve growth factor in bladder dysfunction: contributing factor, biomarker, and therapeutic target . Neurourology and Urodynamics 30: 1227–1241, 2011.
Ochodnický P, Michel MB, Butter JJ, Seth J, Panicker JN, Michel MC. Bradykinin modulates spontaneous nerve growth factor production and stretch-induced ATP release in human urothelium. Pharmacol Res 70(1): 147-54, 2013.
Shahzad A, Knapp M, Lang I, Köhler G. Interleukin 8 (IL-8) - a universal biomarker? Int Arch Med 15: 3-11, 2010.
Springer TA. Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm. Cell 76: 301-14, 1994
Tielen P, Narten M, Rosin N, Biegler I, Haddad I, Hogardt M, Neubauer R, Schobert M, Wiehlmann L, Jahn D. Genotypic and phenotypic characterization of Pseudomonas aeruginosa isolates from urinary tract infections. Int J Med Microbiol 301: 282–292, 2011.
Vlaskovska M, Kasakov L, Rong W, Bodin P, Bardini M, Cockayne DA, Ford AP, Burnstock G. P2X3 knock-out mice reveal a major sensory role for urothelially released ATP Journal of Neuroscience 21: 5670–5677, 2001.
Yoshida M1, Inadome A, Maeda Y, Satoji Y, Masunaga K, Sugiyama Y, Murakami S. Non-neuronal cholinergic system in human bladder urothelium. Urology 67: 425–430, 2006.
Zarogoulidis P, Katsikogianni F, Tsiouda T, Sakkas A, Katsikogiannis N, Zarogoulidis K. Interleukin-8 and interleukin-17 for cancer. Cancer Invest 32(5): 197-205, 2014.