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LPS Treatment and Exposure to PEMF induce Cell Death and Change in Secretory Activity of HMVEC-Bd with MM6 Cocultutre
Advances in Bioscience and Bioengineering
Volume 2, Issue 3, June 2014, Pages: 30-36
Received: Aug. 27, 2014; Accepted: Sep. 11, 2014; Published: Sep. 30, 2014
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Authors
Kaszuba-Zwoińska Jolanta, Department of Pathophysiology, Medical College, Jagiellonian University, Cracow, Poland
Chorobik Paulina, Department of Immunology, Medical College, Jagiellonian University, Cracow, Poland
Nowak Bernadeta, Department of Immunology, Medical College, Jagiellonian University, Cracow, Poland
Ziomber Agata, Department of Pathophysiology, Medical College, Jagiellonian University, Cracow, Poland
Juszczak Kajetan, Department of Pathophysiology, Medical College, Jagiellonian University, Cracow, Poland
Zaraska Wiesław, Institute of Electron Technology, Cracow, Poland
Thor Piotr, Department of Pathophysiology, Medical College, Jagiellonian University, Cracow, Poland
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Abstract
Objective: Present studies are aimed to elucidate that pulsed electromagnetic field (PEMF) influences cell death parameters and cellular interactions in coculture model in response to inflammatory stimulus like E.coli endotoxin. Methods: We hypothesized that PEMF exposure will affect cell death rate in the experimental coculture model, composed of the human bladder microvascular endothelial cell line (HMEVEC-Bd) and MonoMac6 (MM6) cells previously activated with LPS, and exposed to PEMF (7Hz, 30mT) for three times with 24h intervals. Following the last electromagnetic exposure, we measured viability of cocultured and cultured cells by annexin V (AnV) - propidium iodide (PI) flow cytometry staining procedure to evaluate cell death parameters. The level of proinflammatory cytokine, cell adhesion molecules and vascular endothelial growth factor (IL-8, ICAM-1 and VEGF-A) was estimated by ELISA method in coculture and cell culture collected supernatants. Results: PEMF exposure of HMVEC-Bd and MM6 coculture caused decrease of measured cell death parameters (early and late apoptosis as well as necrosis) and diminished production of some inflammatory agents released in response to LPS activation, comparing to not stimulated with PEMF controls. Conclussion: Obtained results confirmed our hypothesis and showed out that PEMF exposure of HMVEC-Bd & MM6 coculture previously activated with LPS exerted an anti-inflammatory effect.
Keywords
Urothelial Cell Line, Monocytic Cell Line, Coculture, Apoptosis, Necrosis, Flow Cytometry, ICAM-1, IL-8,Pulsed Electromagnetic Field, VEGF-A
To cite this article
Kaszuba-Zwoińska Jolanta, Chorobik Paulina, Nowak Bernadeta, Ziomber Agata, Juszczak Kajetan, Zaraska Wiesław, Thor Piotr, LPS Treatment and Exposure to PEMF induce Cell Death and Change in Secretory Activity of HMVEC-Bd with MM6 Cocultutre, Advances in Bioscience and Bioengineering. Vol. 2, No. 3, 2014, pp. 30-36. doi: 10.11648/j.abb.20140203.12
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